预应力钢筋混凝土T梁桥造价分析与应用

文章根据某省不同地形的高速公路采用预应力钢筋混凝土 T 梁桥结构的造价组成的统计分析， 即对分部工程造价及工程量组成进行分析、 对主要影响技术经济指标的基础工程、 上部构造、 下部构造单位桥梁面积的主要材料消耗和材料价格进行了详细分析， 以及材料价格的涨跌对预应力钢筋混凝土 T 梁桥造价技术经济指标的敏感性， 作为今后在高速公路前期工作中对该类结构快速估算投资提供参考依据。     

情景分析法在交通规划中的应用研究

运用情景分析法,综合考虑影响交通发展的5个主要因素,结合专家群体的知识与实际数据资料、各种信息与计算机技术以及数学模型与决策者的经验智慧,依次得到     

兔肾VX2移植瘤模型的建立及其超声显像检测

目的比较建立兔肾VX2移植瘤模型的不同方法的优劣。方法分别用瘤液穿刺法、瘤块液种植法、瘤块包埋法建立兔肾VX2癌模型。采用超声技术动态检测肿瘤体积及CDFI的变化,确定该模型最佳实验干预时段及其血流参数范围。比较各自的成瘤率;观测肾脏肿瘤的大小及其回声、瘤内血管生成以及血流阻抗等指标。结果瘤块包埋法可建立稳定的兔肾VX2移植瘤模型。B超可以实时、准确、方便的监测肿瘤体积及血流动力学变化。结论兔肾VX2移植瘤是一种造模方法简便且成瘤率高的肾癌动物模型,采用超声技术能精确、无创的动态监测肿瘤体积变化及血流特征。     

水泥抗压强度能力验证统计与技术分析

通过对各参加能力验证实验室的水泥抗压强度结果进行稳健统计分析,根据稳健Z比分数客观评价实验室检测能力,反映实验室的检验技术水平,并进一步对导致验证结果出现偏差的因素进行技术分析。     

The β-amyloid protein induces S100β expression in rat hippocampus through a mechanism that involves IL-1

Objective To explore the effect of β-amyloid protein (Aβ) on S100β expression in rat hippocampus and its mechanisms. Methods At 7 days after bilateral stereotaxis injection of different dose of fibrillar Aβ 25-35 and interluekin-1 receptor antagonist (IL-1ra) into the rat CA1 region, the learning and memory abilities of rats were tested with passive avoidance task. Amyloid deposition was detected by using Congo red staining technique. Nissl staining and immunohistochemical techniques were used to analyze the number of neurons, and GFAP and the S100β expression in hippocampal CA1 region , respectively. Results After fibrillar Aβ injection, the step-through latency of rats was significantly shortened compared to that of the control group. The GFAP positive astrocytes were found surrounding amyloid deposition. Neuronal loss occurred in the pyramidal cell layer of CA1 region. The number of S100β positive cells in Aβ-treated group was significantly increased compared with that in the control group. After IL-1ra injection, the number of S100β positive cells was significantly decreased. Conclusion Intrahippocampal injection of Aβ 25-35 could cause similar pathologic changes of Alzheimer's disease. Aβ 25-35 was capable of up-regulating S100β expression in a dose-dependent manner. The injection of IL-1ra could attenuate the effect of Aβ on S100β expression.     

PROLIFERATION AND MIGRATION OF EPENDYMAL CELLS IN THE BRAIN OF ADULT RATS AFTER PERMANENT FOCAL CEREBRAL ISCHEMIA

Objective Ependymal cells are thought to be the primary source of neural stem cells in the adult central nervous system. The purpose of this study is to examine spatial and temporal profiles of ependymal cell proliferation and migration after focal cerebral ischemia. Methods Eighty male Sprague Dawley rats underwent permanent middle cerebral artery occlusion after injection of 10/μL of 0.2% Dil into the lateral ventricle. Rats were sacrificed and brain sections were acquired for pathological evaluation and laser confocal imaging at day 1,3,7,11,14,21 and 28 after ischemia. Results The density of Dil-labeled cells in the ischemic ipsilateral subventricular zone was significantly higher than that in the control group and these labeled cells dispersed in the ischemic ipsilateral subventricular zone and/or were located in ependyma from day 1 to 11. In the ischemic ipsilateral cortex, some Diilabeled cells occurred in peri-infarction and infarction of parietal region at day14 and peaked at day 21 when some Dil-labeled cell nodules were found in this region. During postischemic day 14--28, a significant decrease in labeled celldensity in the ischemic ipsilateral subventricular zone was coincident with a significant increase in labeled cells density in the cortex (peri-infarction and infarction). Conclusion The results indicate that ependymal cells proliferate and migrate after focal cerebral ischemia in the adult rat brain.     

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通过对一次制动模式曲线列控方式的剖析，阐述了在确定列车追踪时间间隔时传统公式使用平均速度进行计算所存在的弊端，从而提出了利用计算机仿真技术对一次模式曲线列控方式下的追踪时间间隔及其对应模式曲线的生成进行模拟计算，并针对该模拟计算提出了相应的模型与算法；同时，基于追踪时间间隔一般情况下受限于车站到达间隔的事实，对列车进站进行了提前减速的优化设计，并提出了相应的模型流程。最后，针对前述研究作了实例验算。     

我国交通事故现状及预防对策研究

对比分析了我国与发达国家交通事故状况，分析我国交通事故的特点，提出了预防和减少我国交通事故的对策，希冀促进我国道路交通安全水平的提高。     

单纯疱疹病毒2型gG-2"独特区"基因的原核表达及其血清学诊断价值

目的在大肠杆菌中表达HSV-2的gG-2"独特区"基因(gG-2T)并对其免疫学特性进行初步研究。方法克隆HSV-2的gG-2T,并在大肠杆菌中表达gG-2T融合蛋白。通过Western blot和间接ELISA鉴定融合蛋白的活性。结果通过原核系统表达的gG-2T融合蛋白可以与GST多克隆抗体结合并在46 ku附近出现特异性结合条带。并且能够与确诊的HSV-2患者血清反应,而不与HSV-1患者血清和正常人血清反应。结论获得了HSV-2 gG-2T融合蛋白,它可以与GST多克隆抗体特异性结合并且可以同HSV-2型患者血清特异性反应;获得的gG-2T蛋白为研究以gG-2蛋白作为靶区域的诊断试剂盒和疫苗研发打下实验基础。     

区域物流信息平台与资源整合

研究第三方物流提供商利用区域物流信息平台整合社会物流资源的方式 ,将综合物流信息平台分为微观 (企业 )和宏观 (区域 )两个层次 ,着重从区域物流信息平台角度 ,分析研究工商企业、第三方物流企业和政府主管部门等不同主体 ,对区域物流信息的需求及社会物流资源整合要求 ,探讨第三方物流提供商利用区域物流信息平台的功能及其运行机制进行物流资源整合的方式 ,进而提出第三方物流提供商整合物流资源可利用的区域物流信息平台“剧场模型”。根据“剧场模型”,强调第三方物流提供商必须吸收“第四方物流”概念中的供应链方案设计、一体化管理和咨询能力 ,这样才能利用区域物流信息平台提高社会物流资源整合能力 ,最终实现第三方物流提供商整合社会物流资源的思路和实际运作模式