水泥抗压强度能力验证统计与技术分析

通过对各参加能力验证实验室的水泥抗压强度结果进行稳健统计分析,根据稳健Z比分数客观评价实验室检测能力,反映实验室的检验技术水平,并进一步对导致验证结果出现偏差的因素进行技术分析。     

The β-amyloid protein induces S100β expression in rat hippocampus through a mechanism that involves IL-1

Objective To explore the effect of β-amyloid protein (Aβ) on S100β expression in rat hippocampus and its mechanisms. Methods At 7 days after bilateral stereotaxis injection of different dose of fibrillar Aβ 25-35 and interluekin-1 receptor antagonist (IL-1ra) into the rat CA1 region, the learning and memory abilities of rats were tested with passive avoidance task. Amyloid deposition was detected by using Congo red staining technique. Nissl staining and immunohistochemical techniques were used to analyze the number of neurons, and GFAP and the S100β expression in hippocampal CA1 region , respectively. Results After fibrillar Aβ injection, the step-through latency of rats was significantly shortened compared to that of the control group. The GFAP positive astrocytes were found surrounding amyloid deposition. Neuronal loss occurred in the pyramidal cell layer of CA1 region. The number of S100β positive cells in Aβ-treated group was significantly increased compared with that in the control group. After IL-1ra injection, the number of S100β positive cells was significantly decreased. Conclusion Intrahippocampal injection of Aβ 25-35 could cause similar pathologic changes of Alzheimer's disease. Aβ 25-35 was capable of up-regulating S100β expression in a dose-dependent manner. The injection of IL-1ra could attenuate the effect of Aβ on S100β expression.     

PROLIFERATION AND MIGRATION OF EPENDYMAL CELLS IN THE BRAIN OF ADULT RATS AFTER PERMANENT FOCAL CEREBRAL ISCHEMIA

Objective Ependymal cells are thought to be the primary source of neural stem cells in the adult central nervous system. The purpose of this study is to examine spatial and temporal profiles of ependymal cell proliferation and migration after focal cerebral ischemia. Methods Eighty male Sprague Dawley rats underwent permanent middle cerebral artery occlusion after injection of 10/μL of 0.2% Dil into the lateral ventricle. Rats were sacrificed and brain sections were acquired for pathological evaluation and laser confocal imaging at day 1,3,7,11,14,21 and 28 after ischemia. Results The density of Dil-labeled cells in the ischemic ipsilateral subventricular zone was significantly higher than that in the control group and these labeled cells dispersed in the ischemic ipsilateral subventricular zone and/or were located in ependyma from day 1 to 11. In the ischemic ipsilateral cortex, some Diilabeled cells occurred in peri-infarction and infarction of parietal region at day14 and peaked at day 21 when some Dil-labeled cell nodules were found in this region. During postischemic day 14--28, a significant decrease in labeled celldensity in the ischemic ipsilateral subventricular zone was coincident with a significant increase in labeled cells density in the cortex (peri-infarction and infarction). Conclusion The results indicate that ependymal cells proliferate and migrate after focal cerebral ischemia in the adult rat brain.     

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通过对一次制动模式曲线列控方式的剖析，阐述了在确定列车追踪时间间隔时传统公式使用平均速度进行计算所存在的弊端，从而提出了利用计算机仿真技术对一次模式曲线列控方式下的追踪时间间隔及其对应模式曲线的生成进行模拟计算，并针对该模拟计算提出了相应的模型与算法；同时，基于追踪时间间隔一般情况下受限于车站到达间隔的事实，对列车进站进行了提前减速的优化设计，并提出了相应的模型流程。最后，针对前述研究作了实例验算。     

单纯疱疹病毒2型gG-2"独特区"基因的原核表达及其血清学诊断价值

目的在大肠杆菌中表达HSV-2的gG-2"独特区"基因(gG-2T)并对其免疫学特性进行初步研究。方法克隆HSV-2的gG-2T,并在大肠杆菌中表达gG-2T融合蛋白。通过Western blot和间接ELISA鉴定融合蛋白的活性。结果通过原核系统表达的gG-2T融合蛋白可以与GST多克隆抗体结合并在46 ku附近出现特异性结合条带。并且能够与确诊的HSV-2患者血清反应,而不与HSV-1患者血清和正常人血清反应。结论获得了HSV-2 gG-2T融合蛋白,它可以与GST多克隆抗体特异性结合并且可以同HSV-2型患者血清特异性反应;获得的gG-2T蛋白为研究以gG-2蛋白作为靶区域的诊断试剂盒和疫苗研发打下实验基础。     

区域物流信息平台与资源整合

研究第三方物流提供商利用区域物流信息平台整合社会物流资源的方式 ,将综合物流信息平台分为微观 (企业 )和宏观 (区域 )两个层次 ,着重从区域物流信息平台角度 ,分析研究工商企业、第三方物流企业和政府主管部门等不同主体 ,对区域物流信息的需求及社会物流资源整合要求 ,探讨第三方物流提供商利用区域物流信息平台的功能及其运行机制进行物流资源整合的方式 ,进而提出第三方物流提供商整合物流资源可利用的区域物流信息平台“剧场模型”。根据“剧场模型”,强调第三方物流提供商必须吸收“第四方物流”概念中的供应链方案设计、一体化管理和咨询能力 ,这样才能利用区域物流信息平台提高社会物流资源整合能力 ,最终实现第三方物流提供商整合社会物流资源的思路和实际运作模式     

电动转辙机可靠性试验监控系统性能参数测试电路设计

介绍了ＺＤ６型电动转辙机可靠性实验监控系统性能参数测试内容，并给出具体实现的硬件电路。     

电子节气门参数自整定模糊PID控制器仿真研究

分析了以功耗低、力矩大、成本较低的普通直流电机作为驱动电机的电子节气门控制系统结构与工作原理,然后设计了节气门参数自整定模糊PID控制器,在一定程度上解决了直流电机作为节气门驱动电机控制不够精确的问题,并通过仿真证明了其用在电子节气门控制中的可行性。     

内皮依赖性血管舒张与2型糖尿病血管病变的关系

目的　探讨内皮依赖性血管舒张与 2型糖尿病 (2 DM )血管病变的关系及其可能原因。方法　利用彩色多普勒超声对 4 1例 2 DM患者和 12例正常人在反应性充血及含服硝酸甘油前后肱动脉内径进行检测 ,评价不同情况下 2 DM内皮依赖性血管舒张 (△D1% )及非内皮依赖性血管舒张 (△D2 % )反应的变化。结果　无论有无微血管病或动脉粥样硬化 ,所有病例△D1%均较正常人组显著减退 (P <0 0 0 1) ;而△D2 %在所有组间均无显著差异。结论　 2 DM在无大 /微血管病变时 ,内皮依赖性血管舒张功能显著减退 ,此变化可能参与了动脉粥样硬化、糖尿病微血管病变的发生 ;而非内皮依赖性血管舒张功能无变化。     

内河航道桥梁整体顶升适用性研究

以交通运输＂十二五＂规划中的内河航道整治升级为背景,对苏州地区部分干线航道桥梁的桥型进行了统计分析。根据整体顶升对桥梁结构受力变化的影响,探讨了几种桥型在整体顶升过程中桥梁结构的安全适用性。同时,根据航道桥梁整体顶升工程造价的相关计算方法,以苏南运河平望运河大桥为工程背景进行整体顶升投资造价测算,分析了整体顶升技术的经济适用性。结果表明：整体顶升技术在内河航道桥梁改造工程中具有良好的适用性。