沿视平面任意方向上运动模糊图像的几何变换

运动所产生的图像模糊在生活中是常见的,对其进行处理的方法有很多种.常见的一种方法是将这个过程用矩阵的方程表示出来,但是这种方法只适合于求解水平方向上的直线运动模型.对非水平方向上的运动来说,就要对运动图像进行预处理,将其转换到水平方向上,介绍如何对运动方向进行识别及利用几何变换对图像进行处理的过程.     

基于CPLD的数字示波器设计

基于编程逻辑器件CPLD进行数字示波器设计,系统硬件设计选用Altera公司的EPM240型芯片,软件系统使用QuartusⅡ进行设计,整个系统在一个芯片上实现。信号通过快速的ADC进行采集,采集的数据通过CPLD进行存储和处理。测试结果表明,此系统集成度高,测量精确,便于在线升级,具有很大的市场空间。     

解台一号船闸的技术改造

着重介绍京杭运河解台一号船闸的闸首、闸室和靠船墩的技术改造设计,为同类船闸的改造提供了一个成功的范例。     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.     

梅观高速公路扩建设计要点分析

结合工程实际,以深圳市梅观高速公路扩建工程为例,对梅观高速公路的设计概况、难点以及环境保护等问题进行论述,对工程实践具有指导意义。     

国有小型运输企业股份合作制改造的思考

对国有小型运输企业实行股份合作制进行了探讨     

Implementation of discharging/charging current sensorless state-of-charge estimator reflecting cell-to-cell variations in lithium-ion series battery packs

This paper offers novel insights to the design and implementation of an innovative state-of-charge (SOC) estimator for the lithium-ion (Li-Ion) series battery pack. The most interesting feature of this approach is that it can utilize information from each filtered terminal voltage of the Li-Ion cells connected in series for SOC estimation of the battery pack. Without actual sensing each discharging/charging current (DCC) applied to the Li-Ion cells, it is possible to extract each DCC estimation from the corresponding filtered terminal voltages with an equivalent electrical circuit model (EECM) identification of all Li-Ion cells in the battery pack. There are two advantages to SOC estimation of the battery pack with this approach. First, the proposal can be implemented simply and effectively, reducing the computational steps required for SOC estimation. By reducing computational steps, the proposal is expected to be more cost-effective. Second, the approach guarantees an improved SOC performance, even if the battery pack results in inevitable cell-to-cell variation among Li-Ion cells. Accordingly, there are fewer differences to previously estimated DCCs among Li-Ion cells. Specifically, all values from the estimated DCCs are properly compensated for by simultaneous parameter modification according to each cell’s electrochemical characteristics. Experimental results clearly demonstrate that our DCC sensorless SOC estimator provides robust SOC performance for the battery pack. This approach considered an experimental battery pack (12S1P) connected in series using 2.6 Ah LiCoO2 cells produced by Samsung SDI.     

发动机振动信号不对称现象分析

某柴油机振动信号出现不对称现象,其最大值与最小值差异较大。该信号的频谱特性表明:在0-100 Hz频段内,存在发动机转速的2阶、4阶、6阶分量,其中2、4阶分量之间存在某种微妙的相位关系,导致其在时域叠加后出现不对称现象。本文进一步研究了2阶、4阶分量在800~3 000 r/min整个转速范围内的振动位移,发现低转速时比高转速时大87.5%以上。根据发动机力学模型认为这是由发动机倾覆力矩引起的,指明了改进方向,为该发动机的改进方案提供了检验依据。     

电磁阀故障切断报警系统

介绍电磁阀故障切断报警系统的原理和结构,重点描述了电磁阀短路保护装置、CAN通讯管理机构和上位监测机,同时介绍了该系统在本森维尔车站的使用原理和效果.