新型抗ErbB2、抗CD16三价双特异性抗体的构建及功能鉴定

目的构建一种新型三价抗ErbB2、抗CD16 BsAb。方法构建重组载体pET22b( )/BsAb;转化大肠杆菌BL21(DE3),获得重组蛋白的表达,通过包涵体复性纯化蛋白。应用悬浮培养系统扩增稳定表达抗人CD16 scFv-Fc融合蛋白的CHO细胞株(CG5细胞)和稳定表达抗人ErbB2 scFv-Fc融合蛋白的CHO细胞株(HG2细胞),通过rProtein A Sepharose Fast Flow亲和层析柱纯化融合蛋白,应用流式细胞术分析BsAb的结合能力。结果该BsAb可在大肠杆菌BL21(DE3)中以包涵体形式高效表达,通过对重组蛋白的复性可获得高纯度的蛋白;复性后的BsAb具有与其亲本抗体相似性的结合靶抗原的能力。结论新型三价抗ErbB2、抗CD16 BsAb能与高表达ErbB2的乳腺癌细胞系SKBR3细胞高亲和力结合和表达CD16的人外周血单个核细胞低亲和力结合。     

射频热凝靶点消融术在颈腰椎间盘突出症中的应用

目的观察射频热凝靶点消融术在治疗颈腰椎间盘突出症中的疗效及安全性,并对该新技术的推广应用做出评估。方法对120例经CT或MRI检查确诊的颈腰椎间盘突出症患者,根据临床症状、CT或MRI结果确定射频热凝的靶点位置,在C型臂引导下穿刺到靶点,通过椎间盘造影确定电极已进入靶点,再行阻抗测试、电生理测试及温度测试后,给予86-94℃温度,对靶点进行热凝消融。此时,通常可以复制出原有的腰腿痛症状,用此温度治疗4个周期,每个周期100 s。结果本组120例患者,经过该技术治疗后,其中73例治疗效果达优级,30例治疗效果良好,13例效果较差,4例无效,优良率85.8%。有4例患者术后并发轻微头痛、头晕等不适症状,均于2日内消失。余无其他并发症。结论射频热凝靶点消融术是目前治疗腰椎间盘突出症的最新的、且安全有效的方法。     

成都地铁1号线盾构掘进对建筑物安全的影响分析

成都地铁1号线一期工程沿成都市中心通过,街道两侧楼房林立;又因该段街道较窄,两侧大部分建筑均在地铁开挖影响范围之内.文章对地铁盾构下穿建筑物时对建筑物的影响特性进行了分析,并参照国外规范及国内实践,提出建筑物损害程度及风险等级标准,可供成都地铁一号线建筑物安全监测及影响评估参考.     

轮缘驱动无轴推进器冷却方案设计与多物理场耦合计算

针对轮缘驱动无轴推进器中集成化永磁电机的散热问题,设计了自然循环的海水冷方案,即在无轴推进器桨叶的前、后端开槽,并与电机定、转子间的气隙连通,形成闭合的、与推进器进出流方向相反的自然水循环通道,冷却通道的流量由推进器桨叶前后截面的压力差、气隙的高度等参数决定。文章基于有限元方法计算了永磁推进电机的电磁性能,并采用有限体积法对轮缘驱动式集成化永磁电机推进器的冷却方案进行了电磁场、温度场与流场的耦合计算,校验了集成化永磁推进电机冷却方案的有效性。     

绿色混凝土生态防护技术在岩质边坡中的应用

介绍了绿色混凝土生态防护技术的原理、工艺和养护管理,并与传统的边坡防护比较,表明其技术具有稳定边坡、美化环境及降低成本等优点。     

基于小波能谱系数的声发射源特征提取方法研究

研究了基于小波能谱系数的声发射源特征提取方法,利用小波变换对典型声发射源的波形信号进行了分析,获取其频域特征.采用小波能谱系数描述信号能量在每个频率范围的分布情况,以区别各种不同的声发射源.利用该方法对港口链斗卸船机现场测试获取的裂纹声发射信号进行了小波分析和特征提取,结果表明,该方法能合理地描述缺陷的声发射特征.     

舰船电力系统潮流计算方法

根据舰船电力系统的特点,在比较陆用电力系统各种潮流计算方法的基础上,提出了适合于舰船电力系统的潮流计算方法.该方法从回推/前推算法出发,利用解环点处电流补偿技术处理弱环网运行状态,采用PV节点电流补偿技术解决系统多源问题,最终得到舰船电力系统潮流统一计算流程.文中给出了计算实例.     

舰船电力系统矩形汇流排集肤效应的数值计算

对舰船电力系统矩形汇流排的集肤效应进行了研究,通过理论分析和数值计算推导了求解矩形导体电流密度的数值方法,并给出了求解电流密度的矩阵表达式.对5 mm宽的矩形铜制汇流排的集肤效应进行了仿真计算,其结果与试验结果一致,证明了文中数值方法的可行性及精确性.     

Analysis of the performance of the evaporator of automotive air conditioning system

The purpose of this research was to establish a theoretical model for the evaporator of automotive air conditioning system and conducting simulations to evaluate the effect of operation parameters, environmental conditions, and design parameters on the performance of evaporator. An automotive air conditioning system primarily consists of four components: the compressor, the condenser, the refrigerant controller, and the evaporator. The refrigerant flow in the evaporator can be divided into two regions: the evaporating region and the superheat region. The refrigerant in the first region is a two-phase flow, while the refrigerant in the latter region is in the state of superheated vapor. The air flowing through the interior of the evaporator can also be divided into two zones: the unsaturated zone and the saturated zone. Water vapor is condensed in the saturated zone while in the unsaturated zone, no water condenses. Because the refrigerant flow and the airflow are perpendicular to each other, the distribution of refrigerant in the evaporating region and the superheat region does not coincide with the distribution of air in the unsaturated zone and the saturated zone. This study examines the effects of different design parameters, environmental conditions and operating parameters on the cooling capacity and superheat of an air conditioning system. Design parameters include the length of the refrigerant channel, the length of the air channel, and the thickness of the fins. Environmental conditions include the air inlet temperature and absolute humidity. Operation conditions include the refrigerant inlet enthalpy, inlet air flow rate, and refrigerant mass flow rate. Results of simulation demonstrated that fins with 50 micron meters width has the greatest cooling capacity for identical outer dimensions; thicker or thinner fins only decreased cooling capacity. Under different outer dimensions, longer refrigerant tubes and air channels created a greater cooling capacity. However, the increase in cooling capacity becomes less and less if the refrigerant flow was fixed because the heat transfer capability of the gaseous refrigerant was limited. In this study, an increase of 19% in cooling capacity can be reached as the length of refrigerant channels was increased, and the increased length of the air channels can promote the cooling capacity by 22%. Besides, it was found in this study that a decrease in the refrigerant inlet enthalpy, the inlet air flow rate, the air inlet temperature, and the inlet absolute humidity, or an increase in the refrigerant mass flow rate, would extend the superheat region and decrease the refrigerant’s superheat. It was also found that the cooling capacity of air conditioners is extremely sensitive to changes in the refrigerant mass flow rate and the inlet enthalpy, and variations more than 50% were found in the operating ranges examined in this study. However, changes in the inlet temperature, absolute humidity, and inlet air flow rate only resulted in variations between 10% and 20% in the examined ranges of conditions. Finally, a correlation among these variables and the simulated cooling capacity was obtained in this study, enabling the relevant researchers to evaluate automotive air conditioning performance under different environmental conditions and operation parameters more easily.     

基于Matlab的混联式混合动力电动汽车的行星齿轮模型分析

利用在Matlab对混联式混合动力汽车进行建模仿真,将整车分为5个子模型,分别是车辆动力学模型、行星齿轮模型、发动机模型、能量管理模型和电力驱动模型,并对混合动力汽车动力系统的动力耦合部件——行星齿轮机构进行了仿真分析。