无水乙醇肾动脉栓塞的并发症

报告无水乙醇肾动脉栓塞治疗肾肿瘤18例的临床并发症,简要讨论了与并发症有关的因素和预防措施。     

Five-axis interpolation of continuous short linear trajectories for 3[PP]S-<Emphasis Type="Italic">XY</Emphasis> hybrid mechanism by dual Bezier blending

A novel five-axis real-time interpolation algorithm for 3[PP]S-XY hybrid mechanism is proposed in this paper. In the algorithm, the five-axis tool path for controlling this hybrid mechanism is separated into two sub-paths. One sub-path describes the movement of 3[PP]S parallel kinematic mechanism module, and the other one describes the movement of XY platform. A pair of cubic Bezier curves is employed to smooth the corners in those two sub-paths. Based on the homogenous Jacobian matrix of 3[PP]S mechanism, a relationship between the position errors of every driving joint in hybrid mechanism and the position deviation of the tool tip center point at the moving platform is established. This relationship is used to estimate the approximation error for the corners smoothing according to the accuracy requirement of tool tip center in interpolation. Due to the high computational efficiency of this corner smoothing method, it is integrated into the look-ahead module of computer numerical control (CNC) system to perform online tool path smoothing. By performing the speed planning based on a floating window scheme, a jerk limited S-shape speed profile can be generated efficiently. On this basis, a realtime look-ahead scheme, which is comprised of path-smoothing and feedrate scheduling, is developed to acquire a speed profile with smooth acceleration. A monotonic cubic spline is employed for synchronization between those two smoothed sub-paths in tool path interpolation. This interpolation algorithm has been integrated into our own developed CNC system to control a 3PRS-XY experimental instrument (P, R and S standing for prismatic, revolute and spherical, respectively). A club shaped trajectory is adopted to verify the smoothness and efficiency of the five-axis interpolator for hybrid mechanism control.     

Study on lift-up speed of aerodynamic compliant foil thrust bearings

Objective The experimental study on the lift-up speed of a new kind of compliant aerodynamic foil thrust hearings was performed on the multifunetional test rig established for testing the performances of foil gas bearings. Methods The lift-up speed of foil gas thrust hearing under given axial load was analyzed through the spectrum of axial displacement response in frequency domain. Results The test results indicated that the difference in the spectrum of axial displacement responses before and after lifting up of the rotor was obvious. After lifting up of the rotor, there were only larger components of rotation frequency and lower harmanie frequencies. If the rotor wasn＇t lift-up, there were also larger components of other frequencies in the spectrum. Conclusion So by analyzing the spectrum of axial displacement response, the results showed that the lift-up speed was about 1 860 rpm when the axial load was 31N.     

T-2毒素对胎儿软骨细胞凋亡、Bcl-2和Bax表达的影响及硒的保护作用

目的　探讨T 2毒素对软骨细胞凋亡的作用,和对凋亡调控蛋白Bcl 2 与Bax表达的影响,以及微量元素硒对软骨细胞的保护作用。方法　采用电镜,Annexin V/PI法检测T 2 毒素致人软骨细胞凋亡的情况,采用流式细胞仪检测凋亡相关蛋白Bcl 2和Bax的表达。结果　T 2 毒素可引起软骨细胞发生凋亡的典型电镜形态改变;不同浓度(1~20μg/L)的T 2毒素均可以引起软骨细胞早期凋亡率和晚期凋亡率明显增加,且在一定范围内呈浓度依赖性,T 2毒素浓度为20μg/L时早期凋亡率最高达5.60%,晚期凋亡率最高达8.61%。生理浓度的微量元素硒可部分减弱T 2毒素引起的凋亡;不同浓度(1~20μg/L)的T 2毒素都能引起Bcl 2和Bax表达水平提高,浓度为10~20μg/L时,Bax/Bcl 2比值升高;硒能部分对抗T 2 毒素引起的Bcl 2 和Bax 表达的提高,使Bax/Bcl 2 比值下降,以降低凋亡率。结论　T 2毒素在浓度为1 ~ 20μg/L 时,可以引起软骨细胞凋亡; T 2 毒素引起的软骨细胞凋亡与软骨细胞内Bax/Bcl 2比值升高有关,硒对T 2毒素引起的软骨细胞凋亡有一定保护作用。     

低温稀土渗硼研究

研究了稀土元素对固体渗硼的作用，测试了４５钢低温渗硼层的组织与性能．结果表明：加适量的稀土元素可显著的提高渗硼速度，低温渗硼层是由ＦｅＢ、Ｆｅ２Ｂ和Ｆｅ３（Ｃ２Ｂ）等相组成．其渗层组织致密、无明显疏松及孔洞，渗硼层前沿过渡区较窄，有良好的抗磨损特性．     

交通运输应急指挥系统架构研究

交通运输突发灾害事件对社会和谐稳定、人民群众生命财产安全造成了巨大危害,如何科学有效地防患、处置交通突发事件,成为交通行业亟待解决的现实问题。该文从交通运输应急指挥目标入手,深入研究交通应急指挥系统的层次结构,完善系统的功能,建立健全相关标准体系,从而提升交通应急反应速度,增强防灾灭灾能力,有效地预防和处置突发事件造成...     

β-淀粉样蛋白增加基底前脑神经元对谷氨酸的易感性

目的探讨β-淀粉样蛋白25-35(Aβ25-35)与谷氨酸(Glu)联合应用,对原代培养的大鼠基底前脑神经元的作用。方法原代培养大鼠基底前脑神经元,应用形态学、MTT法结合ChAT免疫组织化学染色,分别观察Aβ25-35与Glu联合应用,对原代培养的基底前脑神经元存活率、形态学及ChAT免疫反应阳性神经元的影响。结果将100 nmol/L、1μmol/L Aβ分别和20μmol/L Glu联合用于培养的基底前脑神经元,在倒置显微镜下观察,细胞表面粗糙,立体感减弱,胞浆中出现深色颗粒,细胞肿胀或收缩;尼氏染色显示尼氏体减少;MTT检测显示神经元存活率明显下降,100nmol/L Aβ+20μmol/L Glu组与100 nmol/L Aβ组间,1μmol/L Aβ+20μmol/L Glu组与1μmol/L Aβ组间比较均具有统计学差异(P<0.05);ChAT免疫阳性神经元的灰度和截面积均减小。结论Aβ25-35可增加神经元对Glu神经毒性作用的敏感性,表明Glu在阿尔茨海默病发病中起着非常关键的作用。     

面向健康诊断的悬索桥试验模型设计

介绍了以健康监测与诊断研究为目的的悬索桥结构模型试验设计。根据悬索桥的结构特点,建立了考虑横向联结系的三维有限元模型;然后采用ANSYS有限元分析程序对模型的动力特性进行了预分析,获得了模型的自振频率和振型,进一步验证模型试验的可行性,为进行更深层次的试验研究奠定了基础。     

路面自应力破冰铺装结构可行性探讨

为了有效解决困扰公路养护多年的路面凝冰问题,提出了一种路面自应力破冰铺装结构的设想,运用数值分析方法,对铺装结构的各影响因素进行了分析,并通过破冰试验验证了设计方案的可行性。     

通用腺伴随病毒载体的构建及表达报告基因GFP的实验研究

目的构建基因治疗通用型腺伴随病毒(AAV)载体并检测其转染外源基因作用。方法使用限制性内切酶切除pSSV9int质粒中的AAV病毒Rep和Cap基因元件后,插入了重组腺病毒专用穿梭质粒-pACCMVpLpA的含有CMV启动子、多克隆位点(MCS)和多聚腺苷酸信号(PolyA)的表达盒,构建了重组AAV通用载体质粒pSSHG-CMV。在该质粒MCS插入绿色荧光蛋白(GFP)基因后,使用pSSHG-CMV-GFP、GFP140和pAAV/Ad三种质粒共转染293包装细胞,制备GFP重组AAV。应用斑点杂交实验检测重组病毒滴度,并将该病毒感染新生大鼠NIH3T3细胞,荧光显微镜观察GFP表达。结果重组AAV的滴度在浓缩前可达2×1011cfu/mL,浓缩后可达2×1013cfu/mL,表明成功地构建了重组AAV载体。插入外源基因GFP后,在包装病毒和辅助质粒的联合作用下,能产生具有感染性的重组AAV。感染了重组GFP-AAV的大鼠NIH3T3细胞表达了明显的GFP荧光。结论构建的重组AAV通用载体pSSHG-CMV可转染外源基因,这为进一步的基因治疗奠定了基础。