用~3H和地高辛配基标记TNF—α cDNA探针进行原位杂交的比较

用~3H和地高辛配基标记肿瘤坏死因子-αcDNA探针以及原位杂文技术检测33例子宫颈癌和28例阴道尖锐湿疣活检标本中的TNF mRNA。结果表明,两种标记探针原位杂交结果基本一致,~3H标记探针敏感性稍高于地高辛配基标记探针。但放射性核素探针的固有缺点及非同位素探针的安全、快速、方便使非同位素探针有着日益广泛的应用前景。     

On the perceived justice of urban road pricing: An empirical study in Lyon

Various methods of restricting automobile traffic, by price (tolls) or by quantity (odd/even license plates or limited days of traffic), are tested in a survey (N ～ 400) about attitudes toward traffic restrictions in Lyon, France. Ordered probit models with random-effects panel allow us to estimate the survey respondents’ perceptions of these methods, as well as the roles of individual socio-demographic characteristics in the formation of these perceptions. Both the restriction of automobile traffic and its regulation by congestion (waiting in line) are widely considered unjust by the respondents, regardless of whether they work and whether they are drivers or non-drivers. Their attitudes towards tolls justified by the pollution caused by automobile traffic are less negative. As regards compensation, in addition to emergency vehicles and those that transport people with limited mobility, respondents believe car-pooling ought to benefit of a toll exemption. The support for a reduced rate for low-income users shows a concern for justice to which it will be necessary to respond. The respondents’ socio-professional status, level of education, car use or non-use, and residence inside or outside of the toll zone clearly play a role in their perceptions of these methods of regulation and compensation.     

高速公路服务区新建UTW路面设计

高速公路服务区路面频繁受到车辆施加的水平力的作用,超薄水泥混凝土(UTW)路面抗水平力破坏性能强,但将UTW用于服务区新建路面需要有针对性地进行路面设计.在分析服务区交通特性和UTW受力特性的基础上修正了轴载等效换算公式;引入超细粉煤灰(UFA)等量取代部分水泥,设计出的UTW板水泥混凝土满足结构要求;通过直接剪切试验...     

Between-mode-differences in the value of travel time: Self-selection or strategic behaviour?

Using stated preference survey data, we measure the value of travel time for several transport modes. We find, like many before us, that the value of travel time varies across modes in the opposite direction of what would be the consequence of differences in comfort. We examine three candidate causes for the observed differences: Comfort effects, self-selection and strategic behaviour of respondents. Using experiments with both the current and an alternative mode we find that the differences in the value of travel time are consistent with self-selection and comfort effects. Moreover, respondents having bus as the current or the alternative mode seem not to value comfort differently across modes. Strategic behaviour seems to play no role.     

THE DETECTION OF P53 GENE IN HUMAN CERVICAL CARCINOMA WITH AND WITHOUT HUMAN PAPILLOMAVIRUS INFECTION

ＴＨＥＤＥＴＥＣＴＩＯＮＯＦＰ５３ＧＥＮＥＩＮＨＵＭＡＮＣＥＲＶＩＣＡＬＣＡＲＣＩＮＯＭＡＷＩＴＨＡＮＤＷＩＴＨＯＵＴＨＵＭＡＮＰＡＰＩＬＬＯＭＡＶＩＲＵＳＩＮＦＥＣＴＩＯＮＳｕｎＹｉ（孙毅）；ＳｉＬｕｓｈｅｎｇ（司履生）ＳｕｎＹｉ；ＳｉＬｕｓｈｅｎ...     

圆筒型FPSO在波浪中运动响应特性研究

圆筒型FPSO作为一种新型海洋工程装备,逐渐被应用于全球海洋油气资源的开发。该文以适用于南海深水油气田开发的圆筒型FPSO为研究对象,基于三维势流理论和莫里森（Morison）方程,采用Sesam软件建立圆筒型FPSO的湿表面模型和水动力计算模型,并对圆筒型FPSO在波浪中的频域运动响应特性进行计算,将载重量相近的圆筒型FPSO与船型FPSO的纵摇、横摇和垂荡运动响应进行对比研究,结果表明圆筒型FPSO的运动特性优于船型FPSO。该文同时也对不同船体直径、阻尼板直径、船体吃水以及环境条件重现期下的圆筒型FPSO垂荡运动响应进行研究,研究结果对于圆筒型FPSO的总体设计具有重要指导作用。     

使用WEHI细胞和MTT试验检查单核细胞的细胞毒性

以TNF-α敏感细胞株WEHI克隆13为靶细胞,用MTT比色法代替传统的~(51)Cr释放试验检测单核细胞的细胞毒性,本法有更大的优点,快速、敏感,所需效应细胞量少,更适于临床应用,而且无同位素污染。     

中国高速铁路运输组织

中国高速铁路运输组织复杂,不同速度等级并存,骨干网与城际网相互融合,列车运行组织方式多样。建立符合中国铁路特点的安全高效的运输组织模式,从列车开行方式、调度指挥系统、站车客运组织、设备检修运用、应急处置管理5个方面,分析中国高速铁路运输组织的特点和做法,为管好用好世界上标准最高、规模最大的高速铁路奠定重要基础。     

Resolving the impact of short-term variations in physical processes impacting on the spawning environment of eastern Baltic cod: application of a 3-D hydrodynamic model

Variations in oxygen conditions below the permanent halocline influence the ecosystem of the Baltic Sea through a number of mechanisms. In this study, we examine the effects of physical forcing on variations in the volume of deep oxygenated water suitable for reproductive success of central Baltic cod. Recent research has identified the importance of inflows of saline and oxygenated North Sea water into the Baltic Sea for the recruitment of Baltic cod. However, other processes have been suggested to modify this reproduction volume including variations in timing and volume of terrestrial runoff, variability of the solubility of oxygen due to variations in sea surface temperature as well as the influence of variations in wind stress. In order to examine the latter three mechanisms, we have performed simulations utilizing the Kiel Baltic Sea model for a period of a weak to moderate inflow of North Sea water into the Baltic, modifying wind stress, freshwater runoff and thermal inputs. The model is started from three-dimensional fields of temperature, salinity and oxygen obtained from a previous model run and forced by realistic atmospheric conditions. Results of this realistic reference run were compared to runs with modified meteorological forcing conditions and river runoff.From these simulations, it is apparent that processes other than major Baltic inflows have the potential to alter the reproduction volume of Baltic cod. Low near-surface air temperatures in the North Sea, the Skagerrak/Kattegat area and in the western Baltic influence the water mass properties (high oxygen solubility). Eastward oriented transports of these well-oxygenated highly saline water masses may have a significant positive impact on the Baltic cod reproduction volume in the Bornholm Basin.Finally, we analysed how large scale and local atmospheric forcing conditions are related to the identified major processes affecting the reproduction volume.     

倒虹吸隧道智能排污结构构想与数值分析

为减少甚至解决倒虹吸隧道中污物沉积和堵塞问题，提出一种新型倒虹吸隧道工作井智能排污结构。利用SolidWorks软件对隧道及工作井进行建模和数值模拟优化，并进行物理模型试验验证。研究结果表明： 1）外加辅助块体有助于降低湍流位置，而在一定范围内改变入口处水流流速对引水结构的影响不大； 2）在工作井内加入矩形三角块和在入口区加阶梯双三角块、出口区加矩形三角块是倒虹吸隧道较优的智能排污结构方案； 3）雨水方沟断面尺寸大小会对水流湍流位置和流速分布有所影响，但添加适当的辅助块仍具有较好的智能排污效果。