NT4-Ant-Shepherdin[79-87]融合基因重组载体的构建

目的设计构建携带NT4-Ant-Shepherdin[79-87]的cDNA融合基因的重组载体,为针对Survivin的靶向治疗奠定基础。方法应用非对称引物/模板法、PCR技术制备NT4-Ant-Shepherdin[79-87]cDNA片断,连接于pGEM-T-Easy载体,经克隆测序、酶切后与PBV220/NT4质粒连接;转化感受态细胞E.coliDH5α,亚克隆获得NT4-Ant-Shepherdin[79-87]融合基因。结果克隆出Ant-Shepherdin[79-87]基因,经酶切及测序证实结果正确;连接PBV220/NT4,经克隆、酶切,琼脂糖凝胶电泳证实获得321bp的NT4-Ant-Shepherdin[79-87]目的基因片断。结论通过分子生物学技术成功构建了携带NT4-Ant-Shepherdin[79-87]融合基因的重组载体,为进一步研究针对Survivin的靶向抗肿瘤作用奠定了基础。     

厦门海底隧道涌水涌砂应急预案与处理施工技术

结合厦门海底隧道K11＋856发生突发性涌水涌泥的地质灾害处理情况．详细介绍了该段隧道涌水涌泥治理所采用的应急响应与施工措施．监控量测结果验证了处理方案的正确性．对同类地下工程施工具有一定的借鉴意义。     

A New Proposal of Inverse-Time Characteristics for Overcurrent Relay Based on Microcomputer

The conventional time function of electromechanical relays is hard to coordinate with other relays．In order to promote the application of inverse-time overcurrent relays，a new time function for microprocessor-type relay is proposed．The setting of the trip time for this relay is performed by determining the shortest trip time and the longest trip time，respectively．The results of analysis show that with the new time function，the inverse-time overcurrent relay is easy to coordinate with other relays and has a comparatively shorter trip time，and that the fault happens in the protective zone．     

基于Matlab/GUI的公交发车频率优化软件开发

设计了一种基于Matlab的公交发车频率优化软件,将目前主要的发车频率确定方法集成到软件中,同时包含公交客流断面图绘制和优化曲线绘制功能,为用户提供多维度的决策手段。利用Matlab/GUI组件搭建能够与用户交互、可视化的计算平台,较好地实现了公交发车频率优化一体化集成功能。最后通过昆明44路车跟车调查数据验证了软件的可行性。     

Full Duplex Communications for Next Generation Cellular Networks: The Capacity Gain

Full duplex communication highly improves spectrum efficiency of a wireless communication link. However, when it is applied to a cellular network, the capacity gain from this technology remains unknown. The reason is that full duplex communication changes the aggregate interference experienced by each communication link in cellular networks. In this paper, the capacity gain from full duplex communication is studied for cellular networks of 4G and beyond, where the same frequency channel is adopted in each cell. A two-layer Poisson point process (PPP) is adopted to model the network topology, and stochastic geometry is employed to derive the coverage probability and the average capacity of typical link in a cellular network. On the basis of these derived parameters, the capacity gain from full duplex communication is determined. Numerical results reveal that without mutual interference cancellation (MIC), the capacity gain is small under various power levels; with perfect MIC at base stations, the capacity gain can exceed 60%; with imperfect MIC at base stations, the capacity gain decreases quickly even with a slight drop of MIC performance.     

全新视角高速公路施工中的3D路面摊铺施工技术研究

路面摊铺施工作业是一个高度动态的过程, 施工过程管控极为复杂, 如何应用先进的技术手段实时的对摊铺施工过程进行有效管控, 已成为现代化施工管理人员的共识。 文章通过宁波舟山港主通道项目现场路面摊铺的实际应用, 获取了大量的现场摊铺施工数据, 对采用徕卡 3D 路面摊铺施工技术与传统施工技术在施工工艺、 精度、 质量等方面用全新视角看过程理论进行施工整体评价。     

投标文件编制程序与技巧

介绍投标文件的编制程序,投标文件的编制要求,投标报价的技巧,编写投标文件应注意的几个问题     

预应力混凝土简支T形梁优化设计

对预应力混凝土T梁进行优化设计，选取合理的截面有效高度及预应力筋截面面积，可以提高梁的承载能力，降低工程造价．文中以现行规范为依据，运用拉格朗日乘子法进行优化分析，提出了预应力混凝土T梁的优化设计方法，并推导了具有实用价值的计算公式．     

厚层基材喷射植被护坡在路基生态防护中的应用

结合湖南省常德至张家界高速公路边坡防护的实际工程应用情况,介绍了厚层基材喷射植被护坡在路基边坡生态防护中的应用问题。采用生态防护技术,将坡面防护与生态绿化有机结合起来,在保证防护稳定的同时有效的保证了环境、生态的恢复和景观的统一。     

正己烷致大鼠脂质过氧化及肝细胞DNA损伤的实验研究

目的研究正己烷(n-hexane)对大鼠的脂质过氧化作用和肝细胞DNA损伤的影响。方法40只雄性SD大鼠随机分成5组,即阴性对照组、75、150、300 mg/kg染毒组和阳性对照组,每组8只。经腹腔注射染毒4周后,检测肝组织匀浆超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力,血清还原型谷胱甘肽(GSH)和丙二醛(MDA)的含量;用彗星试验技术(SCGE)检测大鼠肝细胞DNA的损伤。结果大鼠体重随染毒时间而增加,阴性对照组增加最快;随染毒剂量增加,肝组织匀浆中SOD、GSH-Px活力、血清GSH含量明显降低,而血清MDA含量增大,组间比较有显著性差异(P<0.05或P<0.01);肝细胞SCGE检测彗星尾长、尾DNA(%)、尾矩、Olive尾矩均增大,组间比较有显著性差异(P<0.01),尾矩值与染毒剂量作相关分析,相关系数r为0.981,呈正相关(P<0.05)。结论正己烷可引起或增强机体氧自由基反应,导致脂质过氧化损伤和肝细胞DNA损伤。