CORRELATIONS BETWEEN BLOOD PRESSURE AND BODY WEIGHT, SERUM LEPTIN IN HIGH CALORIE DIET-INDUCED OBESE RATS

Objective To examine the change of body weight （BW） and blood pressure （BP） in obese rats, clarify relationships between BP and BW and other factors. Methods Male Spraque-Dawley rats were fed either with normal diet （ND） or high calorie diet （HC） for 20 weeks. BW and BP of tail artery were observed biweekly and tetraweekly respectively; serum leptin and fasting insulin （FINS） were detected by enzyme-linked immunoadsordent assay （ELISA） and radioimmunoassay （RIA） respectively. Fasting plasma glucose （FPG） and free fatty acid（FFA） were measured by conventional means. Results BW, abdominal fat weight （AFW）, ratio of abdominal fat weight to body weight （RF/W）, systolic blood pressure （SBP）, diastolic blood pressure （DBP）, serum levels of leptin and FINS, FPG, FFA increased in the HD group after 20 weeks diet intervention （P〈0.05 or P〈0.01 ）. SBP was strongly correlated with BW, leptin, FINS and FFA （P〈0.05）, DBP was correlated with FFA （ r =0.47, P〈0.05）. In addition, leptin was positively correlated with BW, AFW, RF/W, FINS and FFA （P〈0.05 or P〈0.01 ）. Conclusion In this study of high calorie-diet induced rats, the gain of BW is accompanied by increased BP. The obese rats have hyperleptinemia, hyperinsulinemia, hyperglycemia and dyslipidemia which may have important effects on the development of obesityrelated hypertension. RF/W is the key factor in which affect serum leptin level.     

全血环孢素A浓度荧光偏振免疫法监测及评价

应用特异性单克隆抗体荧光偏振免疫法（ＭＡＦＰＩＡ）对６８例肾移植患者进行３６２次临床环孢素Ａ（ＣｓＡ）全血药物浓度监测。结果表明：合并用药会影响ＣｓＡ全血浓度。术后时间与ＣｓＡ全血浓度有一定关系。ＭＡＦＰＩＡ是一种简便、快速、准确的监测方法，对指导临床鉴别排异与中毒反应以及个体化给药，具有重要意义。     

白血病瘤苗对正常小鼠免疫作用的观察

目的　观察自制的一种白血病瘤苗对C57BL/ 6小鼠一般状况 、抵御FBL 3细胞的攻击及小鼠免疫功能的影响。方法　用自制的白血病疫苗 ,内含灭活的FBL 3细胞 +不完全弗氏佐剂 (incompletefoundadjuvant,IFA) +细胞因子(rIL 2、rIL 6、rGM CSF) ,皮下注射免疫C57BL/ 6小鼠 ,用FBL 3细胞皮下注射攻击小鼠 ,观察小鼠一般状况、成瘤率、瘤块生长情况 ;用病理学方法了解瘤块及疫苗注射部位病理变化 ;用MTT比色法检测小鼠巨噬细胞 (macrophage ,Mφ)及细胞毒性T淋巴细胞 (cytotoxicTlymphocyte ,CTL)杀伤活性。 结果　与对照组相比 ,发现自制成的白血病瘤苗能有效的提高小鼠的细胞免疫功能 ,Mφ及CTL杀伤活性明显提高 ,用FBL 3细胞攻击 ,小鼠成瘤率低 ,成瘤小鼠瘤块生长缓慢 ,瘤周单个核细胞浸润明显 ,瘤块内瘤细胞坏死明显。结论　所用白血病疫苗制备简单 ,疗效肯定 ,副作用小 ,有必要进一步研究     

PROPERTIES OF PROLIFERATION AND DIFFERENTIATION OF NEONATAL RAT RETINAL PROGENITOR CELLS IN VITRO

Objective To investigate the properties of proliferation and differentiation of neonatal rat retinal progenitor cells （RPCs） in vitro. Methods RPCs were isolated from neonatal SD rats neural retina and cultured in DMEM/F12 ＋ N2 with EGF and bFGF （suspension medium ） or 10 % FBS without EGF and bFGF （ differentiation medium）. The cells grew as suspended spheres or adherent monolayers, depending on different culture conditions. The neural stem cells or retinal progenitors, neurons, astrocytes, retinal ganglion cells, rod photoreceptors and the proliferating cells were evaluated with immunofluorescence analysis by Nestin or Pax6, Map2, GFAP, Thy-1, Rhodopsin and BrdU antibodies respectively. Results RPCs could propagate and differentiate in suspension or differentiation medium and express the markers of Nestin （92.86%） or Pax6 （86.75%）, Map2 （38.54%）, GFAP （20.93%）, Thy-1 （27. 66%） and Rhodopsin（13. 33%）in suspension medium; however, Nestin （60.27%）, Pax6 （52%）, Map2 （34.94%）, GFAP （38.17%）, Thy-1 （30.84%） and Rhodopsin （34.67%） in differentiation medium. 96.4 % of the population in the neurospheres was BrdU-positive cells. The cells could spontaneously adherent forming some subspheres and retinal specific cell types. Conclusion Neonatal rat RPCs possess the high degree of proliferation and can differentiate into neurons, astrocytes, retinal ganglion cells and rod photoreceptors in vitro. There are different proportions for RPCs to differentiate into specific cell types.     

苦参素对Hela细胞增殖及凋亡的影响

目的探讨苦参素(Matrine)对体外培养的人宫颈癌Hela细胞增殖及凋亡的影响。方法Hela细胞体外培养,MTT法检测细胞增殖,TUNEL法检测细胞凋亡,免疫组化法检测细胞增殖核抗原(PCNA)蛋白的表达水平。结果与对照组相比,0.5、1.0、1.5、2.0、2.5、3.0g/L苦参素均能显著抑制HeLa细胞生长(P<0.05),药物浓度在0.5-1.5g/L之间时表现出时效和量效关系;苦参素2.0、2.5、3.0g/L与1.5g/L相比,抑制无统计学意义(P>0.05)。与对照组相比,1.0、1.5、2.0g/L苦参素组可见到显著性细胞凋亡(P<0.05);1.5g/L苦参素组作用48h和72h时PCNA蛋白表达显著下调(P<0.05)。结论苦参素可能通过抑制增殖和诱导凋亡的双重途径抑制宫颈癌Hela细胞的生长,苦参素有可能成为治疗子宫颈癌的一种药物。     

公路施工中特殊结构物放样的计算方法

桥梁锥形护坡、曲线斜桥梁板等结构物的施工放样在公路施工中非常重要。本文结合实际工作介绍了这两种特殊结构物施工放样的计算方法及计算过程。方法简单实用，可提高工作效率并保证施工放样的精度。     

连续配筋混凝土路面施工工艺研究

介绍了河北张(家口)—石(家庄)高速公路沥青混凝土与连续配筋混凝土复合式耐久性路面试验路段的工程概况、设计方案和连续配筋混凝土试验段施工工艺,对连续配筋混凝土路面施工技术进行了研究。     

INFLUENCE OF REFRIGERANT DISTRIBUTION ON HEAT TRANSFER IN EVAPORATORS

Invaporcompressionrefrigerationsystem ,adryexpansionevaporatorwithlargecoolingcapacityisusu allydividedintoseveralparallelcoilstoenhancetheheattransferanddecreasethepressuredrop .Itishelpfulthattherefrigerantvapourandliquidaremixedhomoge neouslyinadistributorinstalledattheinletoftheevap oratorandthendistributedintoevaporatorcoilsequally .Inpractice ,however ,theinadequatedesignandmanu factureofdistributorsalwayscauseinsufficientmixingandunequaldistributionoftherefrigerant.Inthiscasethethermost…     

再生水泥混凝土疲劳性能

利用水泥混凝土路面养护维修时产生的废料生产再生产水泥混凝土，不仅降低建成成本，而且能减少环境污染，通过对再生水泥混凝土的疲劳性能试验研究，结果表明再生水泥混凝土的疲劳规律与普通水泥混凝土相似，而且在高应力水平状态下，再生水泥混凝土的疲劳寿命较高，使用再生水泥混凝土修建的水泥混凝土路面，完全能够满足混凝土面板的力学性能要求。     

铁路客车动态管理信息系统研究

在分析现行铁路客车运用、检修管理现状的基础上，提出了实行铁路客车现代化管理的必要性，详细介绍了所开发的铁路客车动态管理信息系统的设计原则、设计方法、主要功能及特点。