Protective effect of glutamine pretreatment on ischemia-reperfusion injury of spinal cord in rabbits

Objective To investigate the effect of glutamine (Gln) on the content of reduced glutathione hormone (GSH) and aminoglutaminic acid (Glu) of spinal cord following ischemia-reperfusion injury. Methods Totally 40 healthy adult male rabbits were randomly divided into five groups: sham-operation group (S group), ischemia-reperfusion injury group (I/R group), low-dose glutamine group (L Gln group), median-dose glutamine group (M Gln group) and high-dose glutamine group (H Gln group). After glutamine preconditioning, the model of spinal cord ischemia-reporfasion injury was established according to Zivin's method. The general status of animals was observed and the changes of Jacobs scoring were recorded in each group. Malondialdehydes (MDA), GSH, Glu and superoxide dismutase (SOD) activity in lumbar spinal cord tissues were determined using chemical colorimetry. The neuron number and deviation rate in spinal cord anterior horn were observed histopathologically. Results There was no significant difference between L Gin group and I/R group in behavior scoring, SOD activity, content of MDA and Glu, neuron number and deviation rate of spinal cord (P>0.05); however, there was a significant difference in GSH content of spinal cord (P<0.05). M Gln group and I/R group differed significantly (P<0.05) in behavior scoring, SOD activity, content of MDA, Glu, GSH, neuron number and deviation rate of spinal cord. Between H Gln group and M Gln group, there was no significant difference in behavior scoring, content of MDA and Glu, SOD activity, neuron number and aberration rate in spinal cord (P>0.05), whereas there was a significant difference in SOD activity and Giu content (P<0.05). Conclusion Pretreatment with medium-dose glutamine has a protective effect on spinal cord ischemia-reporfasion injury in rabbits, which may be related to the maintenance of GSH content, increase of SOD activity and reduction of MDA.     

Effect of ulinastatin on hepatic ischemia-reperfusion injury in rats

Objective To investigate the effect of ulinastatin (UTI) on hepatic ischemia-reperfusion injury in rats. Methods Totally 24 adult Sprague-Dawley rats were randomly divided into 3 groups: sham-operated control group (SO group), ischemia-reperfusion group (I/R group) and ulinastatin group (UTI group). Liver in I/R group underwent 1 h of reperfasion after 30 min of ischemia. In UTI group, UTI (2×104 U/kg) was administered to rats 30 min before modeling. The levels of alanine aminotransferase, aspartate aminotransferase and tumor necrosis factor-alpha (TNF-α) in serum were measured and the levels of nitric oxide and malondialdehyde in liver were determined. The histological changes of liver were observed. Results The levels of alanine aminotransferase, aspartate aminotransferase and TNF-α in serum were significantly increased in I/R group compared with those in UTI group (P<0.05). The levels of nitric oxide and malondialdehyde in liver were significantly higher in I/R group than in UTI group (P<0.05).Histological examination of liver indicated that the damages were more severe in I/R group than in UTI group.Conclusion UTI has the ability to inhibit the production of TNF-α and oxyradical, and ameliorate microcirculatory dysfunction in rats with hepatic ischemia-reperfasion injury.     

基于工学结合的高职模具专业实践教学体系构建

模具行业对技术人员的素质和能力要求越来越高,借鉴外国的先进经验,推进工学结合,改革模具专业实践教学体系,构建科学合理的高职模具专业实践教学体系势在必行。     

移动荷载作用下桥上无砟轨道动力分析

桥上无砟轨道越来越多地应用于高速铁路.目前,桥上无砟轨道结构动力计算理论还不够完善,现采用有限元方法,把移动荷载和轨道结构以及桥梁看成一个系统,对板式无砟轨道在移动荷载作用下的动力性能进行分析,并通过MATLAB编程实现,计算结果符合无砟轨道结构和桥梁基本原理.     

Application of large eddy simulation in the performance study of wave blocker

The configuration and aerodynamic performance of the inlet system are important aspects in the process of installing a gas turbine on a naval vessel. Under the requirements, large eddy simulation （LES） is used to simulate the three-dimensional fluid flow in the wave blocker of a marine inlet filter. The Smagorinsky-Lilly sub-grid model was used to model motions of small-scale structures. During numerical simulation, the SIMPLE algorithm was applied. The central-differencing spatial discretization scheme and the second order accuracy finite difference for the temporal discretization were used. Simulation gives satisfactory distribution of the vorticity fields and turbulent kinetic energy. Compared with the k-ε turbulent model, the results of LES are better for the distribution of parameters. The results of experimental study in a small-scale wind tunnel indicate that numerical calculation has higher accuracy. Therefore, the methods used are worthy of reference and introduction for the design of an inlet system.     

The measure and decision of technical risk in the development of naval vessels

The risks in development of navy vessels were explored, The resources of technical risk in the design and development of vessels were mentioned, It was pointed that technical risk dominated the other risk components. Based on the writers＇ understanding and experience from risk analysis and risk ,nanagement to the development of a navy vessel, four measure indexes of technical risk were presented. The decision criteria and the rules corresponding to the indexes were estahlished by the statistic information,     

Study of the helicopter blade running elevation measurement system

Helicopter blade running elevation measurement is an important measure target in helicopter blade dynamic balance experimentation. The elevation influences the helicopter＇ s security and other performance capabilities. In testing, however, it has been difficult to measure the elevation when the rotor reaches high speeds. To get a simple, fast and highly accurate measurement system, photo electricity technology was applied to measuring the blade running elevation. Discussed is the measurement principle of blade running elevation, tire design of the measurement system and analysis of the measurement precision.     

Sensitivity analysis and dynamic modification of modal parameter in mechanical transmission system

Sensitivity analysis is one of the effective methods in the dynamic modification. The sensitivity of the modal parameters such as the natural frequencies and mode shapes in undamped free vibration of mechanical transmission system is analyzed in this paper. In particular,the sensitivities of the modal parameters to physical parameters of shaft system such as the inertia and stiffness are given. A calculation formula for dynamic modification is presented based on the analysis of modal parameter. With a mechanical transmission system as an example, the sensitivities of natural frequencies and modes shape are calculated and analyzed. Furthermore, the dynamic modification is also carried out and a good result is obtained.     

Market based framework for multiple AUVs cooperation

A“Market” based framework for multiple AUVs team is introduced in this paper. It is a distributed meta-level task allocation framwork. The formulation and the basic concepts of the “Market” such as “goods” and “price” are discussed first, then the basic algorithm of the “auction”. The loosely coupled v-MDTSP tasks are considered as an example of the task allocation mission. A multiple AUV team controller and a detailed algorithm are developed for such applications. The simulation results show that the controller has the advantages such as robustness and low complexity and it can achieve better optimization results than the classical central controller （ such as GA） in some tasks. And the comparison of two different local solvers also implies that we should get the reasonable task allocation even not using the high quality algorithm, which can considerably decrease the cooperation computation.     

Geometric calibration algorithm of polarization camera using planar patterns

Polarization pattern provides additional information besides spectral signatures. It can be used in many applications, such as navigation, defect detection and object identification. A novel polarization camera composed of four synchronized cameras is proposed, and it can realize real-time polarization measurement. This study particularly concentrates on the geometric calibration of the system. The projection model is analyzed and the multi-camera calibration algorithm is proposed. Firstly, each camera is calibrated separately using planar patterns, and then the geometric calibration algorithms are performed. Due to the geometrical constraint, a global optimization method results in smaller estimation uncertainties. A mean rotation error of 0.025° and a mean translation error of 0.26mm are achieved after geometric calibration. The images are rectified to establish a correspondence among cameras and are combined to acquire the polarization measurement. The polarization pattern of the skylight is measured by the system and the results are consistent with the previous studies.