逆转录病毒介导CXCR4-siRNA对前列腺癌细胞体外侵袭能力的影响

目的设计构建趋化因子受体4(CXCR4)的RNA干扰逆转录病毒载体,探讨干扰CXCR4表达对前列腺癌细胞侵袭能力的影响。方法用PCR扩增CXCR4特异性小干扰RNA(smallinterferingRNA,siRNA),转入带有增强型绿色荧光蛋白(EGFP)和启动子U6的Pgensil-1质粒中,再将重组基因片段导入到逆转录病毒真核表达载体PLX-SN中,行限制性酶切及测序鉴定。重组逆转录病毒载体转染包装细胞PA317,获取病毒上清转染前列腺癌细胞,RT-PCR检测CXCR4mRNA表达。Transwell小室侵袭实验检测前列腺癌细胞体外侵袭能力的变化。结果通过酶切及测序鉴定,成功构建了PLXSN/EGFP-U6-siCXCR4。干扰质粒抑制CXCR4mRNA的表达,与空载体组比较,PC-3m、LNCaPsiRNA对CXCR4mRNA的抑制率分别为(84.26±10.20)%和(88.17±11.23)%。Transwell侵袭实验结果显示,干扰组微膜下孔细胞数PC-3m、LNCaP分别为(14.7±3.1)和(18.9±4.2)个,空载体组分别为(46.9±5.3)和(66.7±6.0)个,两者差异显著(P<0.05)。结论PLXSN/EGFP-U6-siCXCR4可以有效地抑制前列腺癌细胞CXCR4mRNA的表达,干扰CXCR4可以显著抑制前列腺癌细胞体外侵袭转移能力。     

基于中文网页的综合联系信息提取系统的设计与实现

为满足用户希望通过搜索引擎查询综合联系信息的需求,设计了基于中文网页中的综合联系信息提取系统。研究与阐述了在搜索引擎返回结果的基础上提取有关联系方面的综合信息(例如邮箱、电话等)的实现方法,并通过构建特有的内置"实体"判断、SV值算法提高系统的提取精度。     

55岁以上自然人群中帕金森病患者认知功能障碍的临床研究

目的　了解 55岁以上自然人群中帕金森病 (Parkinson sdisease ,PD)患者认知功能障碍的发生情况。方法　以流行病学调查资料为基础 ,采用非配对的病例对照研究方法 ,分析 55岁以上自然人群中PD患者认知功能障碍的发生率 ,评估认知功能障碍发生的相对危险性。结果　 55岁以上自然人群中 ,PD组和非帕金森病 (noParkinson sdis ease,NOPD)组认知功能障碍的发生率分别为 2 7.78%、5.55% ;年龄调整的发生率分别为 16.2 0 %、9.87%。与同一自然人群中NOPD组比较 ,PD组发生认知功能障碍的危险性明显增高 ,但主要增加低年龄组 (55～ 74岁 )中老年人发生认知功能障碍的危险性 (OR =8.59,95%CI :3 .13～ 2 3 .80 ) ;而且PD是低年龄中老年人发生认知功能障碍和痴呆的独立危险因素 (OR =6.2 7,95%CI :2 .3 5～ 19.2 7;OR =5.50 ,95%CI :1.52～ 19.94)。年龄调整的MMSE分值的均值PD组明显低于NOPD组 (P <0 .0 0 1)。结论　PD主要增加低年龄 (55～ 74岁 )中老年人认知功能障碍发生的危险性 ,而且PD认知功能障碍是疾病本身所致     

扁铲侧胀试验在地铁工程勘察中的应用

通过对某市地铁勘察工程中扁铲侧胀试验与土工试验成果的对比,发现扁铲侧胀试验得到的土体参数与土工试验参数有很好的相关性,基本能判别土的类型和土体的重度,扁铲侧胀试验得出的场地土的扁铲侧胀模量MDMT和不排水抗剪强度CUDMTs约为相应土工试验压缩模量ESO-0.2s和Cu的1.5倍.     

基于还贷的公路收费费率优化方法研究

受经济发展相对滞后、公路建设资金短缺等客观因素制约,我国的收费公路筹资方式为收费还贷.从还贷的角度,在考虑公路建设方和用户双方利益的前提下,以交通均衡理论和最优化理论为基础,建立均衡状态下的基于还贷的公路收费费率优化的双层规划模型,由此确定优化费率.提出了模型的算法并介绍应用实例.     

股动脉注射琥珀胆碱诱发肌梭传入对膈神经放电的影响

目的 观察肌梭传入活动是否对呼吸运动具有反射性调节作用。方法 实验用63只麻醉、制动、切断双侧颈迷走神经、肌松、人工呼吸的家兔,以膈神经放电作为呼吸观测指标,观察股动脉注射琥珀胆碱(Succinylcholine,Sch)诱发肌梭传入活动对呼吸的影响。结果 ①产生易化作用的有40例(63..49%),其中23例(36..51%)呈吸气延长效应;17例(26..98%)呈抑呼增频效应。产生抑制效应的有11例(17..46%),呈双相反应的4例(6..35%),8例(12..70%)无明显作用。②吸气延长效应主要表现为吸气时程(Ti)延长,呼吸时程(Te)有缩短趋势,但差异无显著性,Ti/Te比值增加,呼吸频率(RF)变化不明显;抑呼增频效应主要表现为Te缩短,Ti变化不明显,Ti/Te比值增加,RF加快。③肌注布比卡因破坏肌梭后,同剂量的Sch使上述呼吸易化效应明显减弱。结论 股动脉注射Sch诱发的肌梭传入活动对呼吸运动具有明显的增强作用。     

异步牵引电动机参数化设计的研究

阐述了运用先进的参数化设计方法和程序设计语言VBA(Visual Basic Application)、基于AutoCAD2000的软件平台开发的异步牵引电动机参数化设计系统,并以JD106电动机为模型进行了具体描述。该系统可以大大缩短设计周期,提高效率。     

Factors associated with bicycle ownership and use: a study of six small U.S. cities

As a means of transportation and as a form of physical activity, bicycling generates benefits to the bicyclist as well as to the community as a whole. Bicycling now accounts for less than 1 percent of all trips for all purposes in the U.S., but evidence from other western countries suggests that under the right conditions, bicycling levels can be significantly higher. Indeed, the experiences of some U.S. cities suggest that it is possible to create conditions conducive to higher levels of bicycling even in the U.S. However, the extent to which bicycle investments have contributed to bicycling levels in these communities has not been rigorously assessed. The purpose of this study is to provide a better understanding of the determinants of bicycle ownership and use as a basis for identifying ways to promote bicycling. A cross-sectional study of six cities was designed to test the importance of bicycle infrastructure and other physical environment factors relative to individual factors and social environment factors, using a nested logit model to examine ownership and use decisions jointly. The results show strong effects of individual attitudes and physical and social environment factors on bicycle ownership and use.     

溃坝模拟的光滑粒子流体动力学方法及其粘性特性(英文)

Smoothed particle hydrodynamics (SPH) is a Lagrangian meshless particle method. It is one of the best method for simulating violent free surface flows in fluids and solving large fluid deformations. Dam breaking is a typical example of these problems. The basis of SPH was reviewed, including some techniques for governing equation resolution, such as the stepping method and the boundary handling method. Then numerical results of a dam breaking simulation were discussed, and the benefits of concepts like artificial viscosity and position correction were analyzed in detail. When compared with dam breaking simulated by the volume of fluid (VOF) method, the wave profile generated by SPH had good agreement, but the pressure had only reasonable agreement. Improving pressure results is clearly an important next step for research.     

船舶溢油监测方法的应用现状及发展趋势

介绍了可见光、红外、紫外、激光荧光、微波及电磁能量吸收等船舶溢油监测传感器的工作原理及优缺点;分析卫星遥感监测、飞机遥感监测、巡逻船监测、VTS监测、CCTV监测、定点监测和浮标监测等7种监测方法的研究、应用现状,并通过对比分析指出今后的发展趋势.