磁通门罗盘的数字信号处理方法

为提高磁通门罗盘的指向精度和稳定性,提出了磁通门罗盘的数字信号处理方法。利用微处理器直接产生激励信号,通过模数转换采集磁通门传感器感应的磁场信号,对所采集的原始信息进行傅里叶变换获取偶次谐波,得到罗盘方位值。计算结果表明:二次谐波的波形幅度在各偶次谐波中最大,其提取精度至少提高5倍,而且采用多个偶次谐波并行使用解算方位的方法,降低了原始噪声,增大了原始信息的利用率,提高了磁通门传感器的灵敏度,因此,数字信号处理方法可行。     

AQP4在COPD支气管黏膜的表达及其与患者肺功能、气道黏膜炎症的相关性

目的通过免疫组化法检测支气管黏膜中水通道蛋白-4(aquaporin-4,AQP4)的表达,观察正常组和慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)组支气管黏膜中AQP4表达与气道黏膜炎症及肺功能的关系,明确AQP4在COPD发生、发展中的作用,为COPD的防治开辟新途径。方法选择正常人和COPD患者各30例,测试肺功能,并行支气管镜检查,取得支气管黏膜标本,用免疫组化法检测AQP4表达,HE染色观察支气管黏膜炎症细胞浸润程度。结果COPD组较对照组AQP4表达减弱有极显著差异(P<0.001)。AQP4表达和FEV1/FVC、FEV1/pred%呈正相关(P<0.001),与支气管黏膜炎症严重程度呈负相关(P<0.001)。结论COPD患者AQP4表达减少可能是其肺功能下降及气道炎症加重的原因之一。临床上可以通过改善AQP4治疗COPD。     

多房棘球绦虫重组Bb-Em14-3-3疫苗的构建及其表达效率

目的构建多房棘球绦虫(Em)重组双歧杆菌(Bb)-Em14-3-3疫苗,并研究Em14-3-3分子在大肠埃希菌BL21(DE3)中的表达效率。方法通过PCR扩增Em14-3-3抗原编码基因;将该基因定向克隆于含有谷胱甘肽-S-转移酶(GST)基因的大肠埃希菌-双歧杆菌穿梭表达载体pGEX-1λT,构建重组质粒pGEX-Em14-3-3;用电穿孔法将该质粒导入Bb,构建多房棘球绦虫重组Bb-Em14-3-3疫苗。经PCR和酶切鉴定后以IPTG诱导表达Em14-3-3/GST融合蛋白;SDS-PAGE及Western blot对表达产物进行鉴定。结果PCR成功扩增出530 bp的Em14-3-3基因;双酶切证实Em14-3-3基因插入pGEX-1λT中;PCR证实重组Bb-Em14-3-3疫苗构建成功;SDS-PAGE及Western blot分析显示重组质粒转化宿主菌在IPTG诱导下高效表达了Em14-3-3/GST融合蛋白,表达效率为23%。结论成功构建了多房棘球绦虫重组Bb-Em14-3-3疫苗,重组质粒pGEX-Em14-3-3在大肠杆菌中获得了高效表达,Western blot结果提示表达出的Em14-3-3重组蛋白具有特异抗原性。     

碎石桩处理高液限土地基的离心试验研究

高液限土是一种对工程有害的特殊土。为了处治高液限土地基,对室内土工离心模型试验进行分析,利用离心机产生离心力场,提高模型土体的体积力,形成人工重力。模拟高液限土地基在碎石桩处理后的固结性状,观测地基模型的变形和沉降。试验结果表明,碎石桩复合地基措施确实能改善地基的受力和变形性状,极大增强地基的稳定性。     

贵阳市公交线两规划方法研究

贵阳是山地城市,城市路网、交通结构与居民出行方式特殊,公交的服务水平、现状存在问题及发展方案有别于其他城市,故传统的公交线网规划方法在贵阳存在一定的局限性.本文通过分析当前运用广泛的层次规划法,结合贵阳公交的现状及发展趋势,在满足居民出行需求的基础上,提出了基于公交客流的线网规划方法.     

Integrated evasive manoeuvre assist for collision mitigation with oncoming vehicles

Development and deployment of steering based collision avoidance systems are made difficult due to the complexity of dealing with oncoming vehicles during the evasive manoeuvre. A method to mitigate the collision risk with oncoming vehicles during such manoeuvres is presented in this work. A point mass analysis of such a scenario is first done to determine the importance of speed for mitigating the collision risk with the oncoming vehicle. A characteristic parameter was identified, which correlates well with the need to increase or decrease speed, in order to reduce the collision risk. This finding was then verified in experiments using a Volvo XC90 test vehicle. A closed-loop longitudinal acceleration controller for collision mitigation with oncoming vehicles is then presented. The longitudinal control is combined with yaw stability control using control allocation to form an integrated controller. Simulations in CarMaker using a validated XC90 vehicle model and the proposed controller showed consistent reductions in the collision risk with the oncoming vehicle.     

古夫隧道软弱围岩普通型机械化配套试验性施工技术

为提高隧道软弱围岩施工安全性和加快施工进度,依托古夫隧道出口段工程,首先通过超前地质预报、监控量测、应力应变测试等判断围岩及掌子面稳定性; 然后通过超前支护、低预应力涨壳式锚杆加固围岩,提高围岩自稳能力; 最后采用普通型机械化配套大断面法施工,对围岩的拱顶沉降、周边收敛、应力应变等进行监测,通过数据分析结果指导现场施工。研究结果表明： 1)隧道软弱围岩普通型机械化配套试验性施工技术可以有效地加快施工进度,保证施工安全; 2)信息化管理可以通过数据分析结果指导现场施工,大大提升隧道施工的管理水平。     

Injection Characteristics of Palm Methyl Ester Blended with Diesel Using Zuech’s Chamber

This research attempts to characterize the injection of palm biodiesel blended with diesel in a Zuech’s chamber. Thailand conventional diesel (mandated blend of biodiesel at 5 % or B5), palm biodiesel (B100) and four other biodiesel blends ratios (B20, B40, B60 and B80) were investigated with single hole injector of 140 and 200 μm diameters, injection pressure of 40 MPa to 160 MPa, constant back pressure of 4.5 MPa and energize time of 2.5 ms. The results show that increasing biodiesel blending ratios leads to longer injection delay, larger injection pressure drop, smaller injection quantity discharge coefficient (C_d) and shorter injection duration. With increasing biodiesel blending ratio, high Cavitation number from biodiesel viscosity decreases Reynolds number. Increasing injector diameter from 140 μm to 200 μm has reduced injection delay, increased fuel injection quantity, discharge coefficient and remaining injection duration. The increasing of injection pressure were improve, injection delay, injection duration, injection quantity and discharge coefficient until injection pressure 120 MPa. In addition at injection pressure over 120 MPa are decrease injection quantity and discharge coefficient, it effect form the cavitation phenomena. Increasing of viscosity, density, Bulk modulus and sound velocity were effect to increase injection delay, with reduce injection quantity, injection duration and pressure drop during injection process.     

Load and Load Dependent Friction Identification and Compensation of Electronic Non-Circular Gear Brake System

Control of the electronic non-circular gear brake (ENGB) involves challenges, including the non-linear variation of loads and the effect of friction, which is dependent upon load. The controller must be designed based on modelling information in order to enhance control performance. This study performed model identification of the ENGB system using a DOB-based model identification method. By employing the nearest neighbor search method, the even-odd disturbance was separated without the influence of hysteresis even in situations with low control precision. The accuracy of the resulting ENGB system model was validated through experiments. The self-energizing effect due to friction between the brake disc and pad within the mechanical system was also validated.     

Numerical Analysis of the Mixture Formation in a Two-Stroke Wall-Guided LPG DI Engine for Extended-Range Electric Vehicle

The use of automotive LPG characteristics which are easy to evaporate vaporization and carry. The paper presents a design of extended-range electric vehicle for wall-guided two stroke LPG engine with direct injection combustion system. Based on the modified vehicle LPG spray model, a database describing the characteristics of vehicle LPG fuel was built and imported into the CFD software. And the accuracy of the model is verified by the Schlieren experimental results. The concentration and velocity field of the mixture in the cylinder under different load conditions are numerically analyzed. The analyzed result indicated that the start injection time θ = 60°–70°CA BTDC under part load condition, the plug electrode near the gathering of a richer mixture is easy to be fired at spark ignition time, the obvious formation of mixture in cylinder is formed and the overall air-fuel ratio is above 40: 1. The start-transition working condition and large load conditions in the piston moves upward before closing the exhaust port to start injection LPG. The optimized LPG injection start time θ ensures that the fresh gas is locked in the cylinder when the exhaust port is closed (63°CA ABDC). In the ignition time of the spark plug, an ideal homogeneous mixture in the cylinder is realized.