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911.
912.
目的设计构建趋化因子受体4(CXCR4)的RNA干扰逆转录病毒载体,探讨干扰CXCR4表达对前列腺癌细胞侵袭能力的影响。方法用PCR扩增CXCR4特异性小干扰RNA(smallinterferingRNA,siRNA),转入带有增强型绿色荧光蛋白(EGFP)和启动子U6的Pgensil-1质粒中,再将重组基因片段导入到逆转录病毒真核表达载体PLX-SN中,行限制性酶切及测序鉴定。重组逆转录病毒载体转染包装细胞PA317,获取病毒上清转染前列腺癌细胞,RT-PCR检测CXCR4mRNA表达。Transwell小室侵袭实验检测前列腺癌细胞体外侵袭能力的变化。结果通过酶切及测序鉴定,成功构建了PLXSN/EGFP-U6-siCXCR4。干扰质粒抑制CXCR4mRNA的表达,与空载体组比较,PC-3m、LNCaPsiRNA对CXCR4mRNA的抑制率分别为(84.26±10.20)%和(88.17±11.23)%。Transwell侵袭实验结果显示,干扰组微膜下孔细胞数PC-3m、LNCaP分别为(14.7±3.1)和(18.9±4.2)个,空载体组分别为(46.9±5.3)和(66.7±6.0)个,两者差异显著(P<0.05)。结论PLXSN/EGFP-U6-siCXCR4可以有效地抑制前列腺癌细胞CXCR4mRNA的表达,干扰CXCR4可以显著抑制前列腺癌细胞体外侵袭转移能力。 相似文献
913.
A series of Pd/Co3O4 catalysts were prepared by Self-Propagating High-Temperature Synthesis (SHS) method in this study, and electric field was applied for catalytic combustion of lean methane over Pd/Co3O4 catalysts at low temperature. When electric field was applied, the catalytic combustion performance of Pd/Co3O4 catalysts was greatly improved, and the application of electric field could reduce the load of active element Pd to some extent while maintaining the same efficiency. Based on experimental tests and the analysis results of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), H2-temperature-programmed reduction (H2-TPR) and in-situ diffuse reflectance infrared Fourier transform spectroscopy (in-situ DRIFTS), the mechanism of catalytic oxidation of CH4 over Pd/Co3O4 catalysts in electric field was proposed. The catalytic combustion of CH4 occurs only when the temperature is higher than 250 °C normally, but when electric field was applied, the whole process of CH4 oxidation was promoted significantly and the reaction temperature was reduced. Electric field could promote the reduction of the support Co3O4 to release the lattice oxygen, resulting in the increase of PdOx and the surface chemisorbed oxygen, which could provide more active sites for the low-temperature oxidation of CH4. Furthermore, electric field could accelerate the dehydroxylation of CoOOH to further enhance the activity of the catalysts. 相似文献
914.
Sepsisischaracterizedbyreleaseofexcessiveamountofproinflammatorycytokinesinthecircu lation ,leadingtoa generalizedanduncontrolledhostresponse .Aconsiderablenumberofexperimen talandclinicalinvestigatorshavereportedemploy inghemofiltration (HF)toeliminatecytokinesandchemokines .Thisexcessiveinflammatoryresponsecouldbedownregulatedwithremovalofcytokinesandothermediators .Thesedatashowedthatthemechanismofmediatorseliminationwaslinkedtoconvectionandadsorptionbyhemofiltration .Ad sorptionappearstob… 相似文献
915.
本文提出了一种分析已有公交专用道运行状态的方法.通过计算公交车道和社会车道上的饱和度,来确定公交车道和社会车道的服务水平,并结合公交专用道上各车型所占比例,分析公交专用道的运行情况及其对社会车道的影响,指出存在的问题和可能的改进措施. 相似文献
916.
提出了在用Arena软件对供应链进行仿真建模研究中的一些思路,通过计算机对供应链系统的仿真分析,不仅可以使得管理人员对由于从销售端到供应端的需求变化引起的“牛鞭效应”进行较为深入和直观的分析,同时也可以在模型中快速对不同物流管理策略和方案进行测评.整个供应链和供应链中各环节的平均库存水平和服务水平是所介绍的模型中的主要供应链绩效指标.文中采用了由供应商、制造商、分销商、零售商和客户所组成的传统供应链结构进行仿真建模,同时介绍了完成的第一个Arena仿真模型. 相似文献
917.
An asynchronous wrapper with novel handshake circuits for data communication in globally asynchronous locally synchronous (GALS) systems is proposed. The handshake circuits include two communication ports and a local clock generator. Two approaches for the implementation of communication ports are presented, one with pure standard cells and the others with Mttller-C elements. The detailed design methodology for GALS systems is given and the circuits are validated with VHDL and circuits simulation in standard CMOS technology. 相似文献
918.
王英林 《上海交通大学学报(英文版)》2016,21(6):692-701
Nowadays, software requirements are still mainly analyzed manually, which has many drawbacks (such as a large amount of labor consumption, inefficiency, and even inaccuracy of the results). The problems are even worse in domain analysis scenarios because a large number of requirements from many users need to be analyzed. In this sense, automatic analysis of software requirements can bring benefits to software companies. For this purpose, we proposed an approach to automatically analyze software requirement specifications (SRSs) and extract the semantic information. In this approach, a machine learning and ontology based semantic role labeling (SRL) method was used. First of all, some common verbs were calculated from SRS documents in the E-commerce domain, and then semantic frames were designed for those verbs. Based on the frames, sentences from SRSs were selected and labeled manually, and the labeled sentences were used as training examples in the machine learning stage. Besides the training examples labeled with semantic roles, external ontology knowledge was used to relieve the data sparsity problem and obtain reliable results. Based on the SemCor and WordNet corpus, the senses of nouns and verbs were identified in a sequential manner through the K-nearest neighbor approach. Then the senses of the verbs were used to identify the frame types. After that, we trained the SRL labeling classifier with the maximum entropy method, in which we added some new features based on word sense, such as the hypernyms and hyponyms of the word senses in the ontology. Experimental results show that this new approach for automatic functional requirements analysis is effective. 相似文献
919.
基于变i法理论的级配组成设计方法 总被引:2,自引:0,他引:2
彭波 《武汉理工大学学报(交通科学与工程版)》2005,29(5):751-754
在对最大密度曲线理论和粒子干涉理论及相关级配算法研究的基础上,提出了变i法及相应算法.通过变i法计算的集料级配与规范中AC-16,SMA-16级配和以粒子干涉理论为依据设计的嵌挤密实结构级配的比较,给出了满足AC-16和SMA-16级配的相应i值范围.结果表明:变i法的提出既考虑了各级粒径以1/2递减的情况,又考虑了各粒级不以1/2递减的级数计算情况,较好地适应了我国沥青混合料级配采用方孔筛的划分标准,更适于沥青混合料级配计算;对AC型及SMA型沥青混合料,给出了各自适宜的i值取值范围;以粒子干涉理论为依据设计的级配与变i法计算的级配相吻合,表明采用变i法设计嵌挤密实结构级配是可行的;路用性能试验结果表明利用变i法理论调整后的沥青混合料具有较高的使用品质,适宜作高等级公路沥青面层. 相似文献
920.
c-myc反义寡核苷酸对肾癌细胞的生长抑制作用及对细胞周期的影响 总被引:8,自引:0,他引:8
目的 观察修饰的c myc反义寡核苷酸 (ASODN)对肾癌细胞的生长和细胞周期的影响。 方法 用人工合成的c mycASODN转染于培养的人肾癌细胞 ,观察其对瘤细胞的作用。结果 ①MTT法测定发现 ,与正义和错配链相比较 ,c mycASODN有明显抑制肾癌细胞生长的作用 ,12 μmol·L-1c mycASODN组作用 4 8h ,对瘤细胞的生长抑制率为 4 7% ;该生长抑制作用随反义药物剂量的增加而增强 ,同时随作用时间的变化而变化。②流式细胞仪检测证明 ,c mycASODN对肾癌细胞周期的抑制作用在G1期到S期。结论 c mycASODN具有序列特异性和细胞周期特异性地抑制肾癌细胞生长作用 相似文献