An Intelligent Master Model of Computer Aided Process Planning for Large Complicated Stampings

Process planning for large complicated stampings is more complicated, illegible and multiform than that for common stampings. In this paper, an intelligent master model of computer aided process planning （CAPP） for large complicated stampings has been developed based on knowledge based engineering （KBE） and feature technology. This innovative model consists of knowledge base （KB）, process control structure （PCS）, process information model （PIM）, multidisciplinary design optimization （MDO）, model link environment （MLE） and simulation engine （SE）, to realize process planning, optimization, simulation and management integrated to complete intelligent CAPP system. In this model, KBE provides knowledge base, open architecture and knowledge reuse ability to deal with the multi-domain and multi-expression of process knowledge, and forms an integrated environment. With PIM, all the knowledge consisting of objects, constraints, cxtmricncc and decision-makings is carried by object-oriented method dynamically for knowledge-reasoning. PCS makes dynamical knowledge modified and updated timely and accordingly. MLE provides scv. cral methods to make CAPP sysmm associated and integrated. SE provides a programmable mechanism to interpret simulation course and result. Meanwhile, collaborative optimization, one method of MDO, is imported to deal with the optimization distributed for multiple purposes. All these make CAPP sysmm integrated and open to other systems, such as dic design and manufacturing system.     

5-氮胞苷对宫颈癌细胞系DAPK1异常甲基化的影响

目的　观察宫颈癌细胞系SiHa及HeLa中凋亡相关蛋白激酶 1(DAPK1)的表达与甲基化修饰之间的相关性,以及5 氮胞苷对细胞DAPK1表达和生长的影响。方法　应用甲基化特异性PCR法分析细胞中DAPK1的甲基化状态;采用RT- PCR及免疫组化SABC法检测不同浓度 5- 氮胞苷干预对宫颈癌细胞 DAPK1 mRNA及蛋白表达的影响;MTT法观察5 -氮胞苷对细胞增殖的影响。结果　DAPK1基因在宫颈癌细胞 SiHa中有甲基化修饰,5- 氮胞苷干预能使其甲基化的DAPK1基因重新表达并抑制细胞生长。结论　DAPK1 的甲基化修饰是宫颈癌发生重要机制之一,5 -氮胞苷能使甲基化的DAPK1去除甲基化修饰,重新表达并恢复抑癌作用。     

Ⅰ型胶原及NF-κB与动脉粥样硬化关系的实验研究

目的探讨动脉粥样硬化(AS)时血脂成分的改变以及Ⅰ型胶原mRNA与其蛋白、NF-κBmRNA与其蛋白在兔AS发生中的作用及其机制,为临床预防及治疗AS斑块提供新的理论依据。方法采用HE、免疫组化及原位杂交法染色,光镜观察AS病变,并应用CMIAS真彩色医学图像分析系统分析;检测40只食饵性AS新西兰兔血脂成分的改变及血管壁的病理变化、Ⅰ型胶原mRNA与其蛋白、NF-κBmRNA与其蛋白的表达情况。结果实验兔15周时血胆固醇(TC)升高约43倍,血低密度脂蛋白(LDL)升高约37倍。主动脉内壁可见脂质条纹形成。主动脉AS病变Ⅰ型胶原、NF-κB蛋白及Ⅰ型胶原mRNA、NF-κBmRNA表达定量(A值)分别为0.27±0.02、0.19±0.01及0.30±0.03、0.35±0.03,显著高于对照组(0.08±0.01、0.09±0.01及0.11±0.01、0.10±0.09,P<0.05)。结论AS时Ⅰ型胶原增多刺激NF-κB的活性,可能参与了血管AS的形成。     

MEDICAL IMAGE SEGMENTATION BASED ON A MODIFIED LEVEL SET ALGORITHM

Imagesegmentationplaysanimportantrolein imageanalysisaswellasinhigh levelimageinter pretationandunderstandingsuchasrobotvision, objectrecognition,andmedicalimaging.Numerous segmentationmethodshavebeendeveloped.How ever,medicalimagesareoftencorruptedbyno…     

辽宁省公路自动化电子收费系统简介

作者根据长期公路收费工作实际,针对普通公路收费的特点,对所研制开发出的辽宁公路自动化电子收费系统的组成及前台收费流程作一简要介绍.     

复合投药混凝法预处理低温生活污水的试验研究

通过对低温生活污水混凝试验得知,采用PAC(聚合铝)与PAM(聚丙烯酰铵)复合投药方式,在低温条件下,比单一混凝药剂具有更高的处理效果.能使CODCr的去除率达到55%以上.此方法在减少投药量,降低运行成本的同时也减轻了后续处理工艺的负担.     

自动引导车辆(AGV)地图生成方法研究

提出了在人工导引方式下生成AGV数字地图的方法.利用立体视觉提取障碍物边界点,将不同视点处得到的观测集成到数字地图中,进一步提高数字地图的精度.由于AGV运动的不确定性,在将最新的观测集成到地图中之前,通过匹配新的观测数据与地图数据计算AGV位置.实验表明了该方法的有效性.