论经济新常态下交通运输行政执法体制改革

交通运输是国民经济基础性行业,经济新常态下改革交通运输执法体制是贯彻中央全面深化改革、全面依法治国方针的内在要求,是消除执法体制机制弊端、根治执法乱象、破解交通运输发展瓶颈的有效途径,改革的主要内容和目的主要有整合职能、精简机构、核定编制、保障经费四个方面。改革中市辖区不设置执法机构,市管公路治超站移交县级管理,妥善进行富余人员转岗分流,要制定配套政策、采取措施保障改革顺利进行,处理好改革与稳定的关系,做到改革与治理两不误。     

基于下游大水位变率的下水式垂直升船机随动技术的设计与实现

下游航道水位变率大,快速适应水位变化的准确停位随动技术,对下水式升船机的传动控制很重要.本文介绍下水式垂直升船机随动控制技术.首先,对控制对象的特性进行分析,描述了系统的速度运行曲线,对位置控制功能进行了研究.然后,搭建试验平台对本控制方案的性能进行了测试,给出了测试的方法和试验结果.实验结果表明本控制系统具有定位精度高、响应速度快等优点,能够适应下游大水位变化率的下水式垂直升船机准确停位要求.     

A proposed vision and vehicle-to-infrastructure communication-based vehicle positioning approach

It is essential to obtain accurate location of vehicles for new applications of Intelligent Transportation Systems. To remedy the defects of present Global Positioning System and vehicle-to-infrastructure (V-I) positioning technology, a new positioning approach based on vision and V-I communication is proposed. This approach aims at lane-level positioning with lower cost than conventional ones. In this approach, the position of the vehicle is represented by its lateral position (the lane number) and longitudinal position (the distance from entrance of the road) in a course coordinate system along the road; the specific lane the vehicle is occupying (the lane number) can be judged using the information of lane lines detected by vision systems; then the distance to the vehicle is obtained by a Road Side Unit (RSU) during the V-I communication; and the longitudinal position is calculated. The error of the approach on typical operating conditions is analyzed, indicating that the new approach can achieve the accuracy of less than 0.31 m for straight road and 0.58 m for typical arc road with ultra-wideband communication and ranging technologies and rational arrangement of RSUs. The feasibility of this approach is presented.     

Approximate optimal AUTOSAR software components deploying approach for automotive E/E system

The AUTOSAR has been developed as the worldwide standard for automotive E/E software systems, making the electronic components of different suppliers to be employed universally. However, as the number of component-based applications in modern automotive embedded systems grows rapidly and the hardware topology becomes increasingly complex, deploying such large number of components in automotive distributed system in manual way is over-dependent on experience of engineers which in turn is time consuming. Furthermore, the resource limitation and scheduling analysis make the problems more complex for developers to find out an approximate optimal deploying approach in system integration. In this paper, we propose a novel method to deploy the AUTOSAR components onto ECUs with the following features. First, a clustering algorithm is designed for deploying components automatically within relatively low time complexity. Second, a fitness function is designed to balance the ECUs load. The goal of our approach is to minimize the communication cost over all the runnable entities while meeting all corresponding timing constraints and balancing all the ECUs load. The experiment results show that our approach is efficient and has well performance by comparing with other existing methods in specific and synthetic data set.     

京津客运专线悬浇连续梁桥面平整度控制技术

结合京津客运专线悬臂浇筑连续梁的施工过程,介绍了主梁的整体线形及各节段间的接缝控制和桥面横向六面排水坡等平整度控制内容,对全桥合拢后桥面的平整度缺陷处理方法进行了阐述,对今后悬浇连续梁施工中的桥面平整度控制与处理有一定的借鉴作用。     

Apoptin原核表达载体的构建及在大肠杆菌中的表达

目的构建Apoptin的原核表达载体,并制备抗原物质Apoptin融合蛋白。方法在获得Apoptin融合基因的基础上,成功构建了Apoptin的高效原核表达载体pET-28a( )-Apoptin,将该质粒转化至大肠杆菌E.coliBL21(DE3)受体菌中,以IPTG对其进行诱导表达,聚丙烯酰胺凝胶电泳分析目的蛋白。结果转化有Apoptin的原核表达载体pET-28a( )-Apoptin的大肠杆菌E.coliBL21(DE3)经IPTG诱导后,经SDS-PAGE分析,在相对分子质量约17 000的位置出现目的蛋白条带,大小与Apoptin融合蛋白一致。结论Apoptin原核表达载体pET-28a( )-Apoptin能够表达出Apoptin融合蛋白,为进一步的Apoptin研究和制备Apoptin抗体奠定了基础。     

幽门螺杆菌尿素通道蛋白基因UreI载体的构建、融合表达及纯化

目的构建幽门螺杆菌(H.pylori,Hp)尿素通道蛋白基因UreI的融合表达载体,并在E.coliBL21中表达,为进一步研究UreI的功能奠定基础。方法利用分子克隆技术以Hp DNA染色体为模板,扩增Hp UreI基因片段,将目的基因UreI与载体pET32a( )分别经kpnⅠ和HindⅢ双酶切,纯化,连接。筛选阳性重组载体,转化大肠杆菌BL21,以IPTG诱导表达pET32a( )/UreI融合蛋白。表达产物经Ni-NTA琼脂糖树脂纯化后,用SDS-PAGE及Western blot分析。结果经酶切、测序分析表明,插入的基因片段为Hp UreI编码基因,由585 bp组成,与GenBank相应菌株HP AM417 609序列完全一致,无碱基突变。经SDS-PAGE分析显示重组质粒pET32a( )/UreI在原核细胞中得到了高效融合表达,其重组融合蛋白分子量为130 ku,经Ni-NTA琼脂糖树脂纯化后,其纯度达95%以上,Western blot分析显示,该重组融合蛋白可被6-His鼠抗单克隆抗体所识别,具有良好的反应原性。结论成功地克隆和构建了原核表达载体pET32a( )/UreI,并在原核细胞中得到了高效融合表达。     

某型号制导电子箱的检测系统设计*

为解决现有导弹检测设备存在的缺陷,设计了一种模块化、通用化、智能化和标准化的综合检测修理平台。论文介绍了系统的硬件组成以及基于Labwindows／CVI的软件开发。通过部队实践证明,该系统具有重大军事效益和经济效益和广泛的运用前景。     

Experimental and numerical investigations on vibration characteristics of a loaded ship model

In this paper, vibration characteristics of the structure in the finite fluid domain are analyzed using a coupled finite element method. The added mass matrix is calculated with finite element method (FEM) by 8-node acoustic fluid elements. Vibration characteristics of the structure in finite fluid domain are calculated combining structure FEM mass matrix. By writing the relevant programs, numerical analysis on vibration characteristics of a submerged cantilever rectangular plate in finite fluid domain and loaded ship model is performed. A modal identification experiment for the loaded ship model in air and in water is conducted and the experiment results verify the reliability of the numerical analysis. The numerical method can be used for further research on vibration characteristics and acoustic radiation problems of the structure in the finite fluid domain.     

藻菌共生体系对镇江市金山湖湖水的异位修复模拟研究

根据镇江市金山湖湖水的水质配制了模拟湖水,考察了小球藻、栅藻、铜绿微囊藻和聚球藻对模拟湖水中总氮(TN)、总磷(TP)和化学需氧量(COD)的去除效果,筛选出小球藻作为优势藻种.文中研究了小球藻的初始密度对模拟湖水中TN、TP和COD去除效果的影响.结果表明:小球藻初始密度为5×106个/m L时对TN、TP和COD的去除效果相对较好,在72 h内去除率分别可达55.1%,96.2%和55%.文中还对藻菌共生体系修复模拟湖水的效果进行了研究,确定了最佳藻菌体积比为1∶2时,TN,TP和COD的去除率分别达到62.4%,97.2%和96.2%.结果表明:藻菌共生体系可有效地对富营养化湖水进行异位修复.