成纤维母细胞造血支持作用的研究

比较了成纤维母细胞 ( Fibroblast)在成人外周血、骨髓和脐带血的不同情况及成纤维母细胞的造血支持作用。结果显示 :1氢化可的松明显促进成纤维母细胞的生长。 2成人外周血中无成纤维母细胞集落形成单位 ( Colony forming unit-fibroblast,CFU- F)。 3骨髓中 CFU- F的数量多于脐带血 ( P <0 .0 1 )。 4骨髓和脐带血中的 CFU- F促进粒系 -单核系祖细胞 ( Granulocyte macrophage colony- formingunit,CFU- GM)集落形成 ,两者比较无明显差异。表明成纤维母细胞的存在很可能有助于粒单系祖细胞的分化和成熟。     

一种基于MSP430F169的消防队员灭火环境监控装置

针对消防队员在灭火过程中经常出现伤亡的问题,设计一种基于MSP430F169芯片、CC2430无线数据通讯模块、JHD161A液晶显示模块以及氧气、一氧化碳、温度、烟雾浓度等传感器等的消防队员灭火环境安全监控装置。该装置与消防指挥中心之间采用无线通讯方式进行数据传输,实现了消防队员灭火现场关键环境参数的实时采集以及消防指挥中心对消防队员所处环境危险程度的有效监控,为有效减少消防队员的伤亡提供了保障。     

姜黄素对食管癌细胞系放射敏感性的影响及其机理

目的　探讨姜黄素对食管癌细胞系Eca10 9的放射增敏作用及其机制。方法　利用细胞克隆技术进行剂量 -存活曲线分析 ,末端脱氧核苷酰转移酶法检测细胞凋亡 ,并对细胞周期进行分析。结果　姜黄素组D0 值为 91.35cGy ,空白对照和空白药物组D0 值分别为 130 .0 7cGy和 138.37cGy ,(P <0 .0 5 )。流式细胞仪分析空白组和姜黄素组凋亡区阳性细胞分别为 1.0 1%和 87.5 8% (P <0 .0 5 ) ;经姜黄素处理后 ,与对照组相比 ,G0 1 期细胞比例降低 ,G2 + M期细胞比例增高。结论　姜黄素可以明显提高Eca 10 9细胞的放射敏感性 ,机制可能与其引起瘤细胞周期阻滞 ,诱导肿瘤细胞凋亡有关     

Identification of differentially expressed genes in two new human bladder carcinoma cell lines

Objective To screen and identify differentially expressed genes in two new human urothelial carcinoma cell lines, BLS-211 and BLX. Methods Suppression subtractive hybridization （SSH） was used to createa subtracted library, and clones were sequenced. Results Totally 13 over-expressed genes in BLX and 9 in BLS-211 cells were obtained, respectively. Among them, 18 were known genes and 4 were new ESTs （Expressed Sequence Tag）, and were collected by GenBank dbEST database （The access number was EB390424-7）. Conclusion SSH is a powerful method for the identification of differentially expressed genes. The differential expression of some BCG-associated genes in different ceils may be related to the different responses to clinical BCG therapy. The identified new ESTs can be cloned for full length to further study their functions.     

THE CONSTRUCTION AND EXPRESSION OF THE MURINE SCFV GENE IN E. COLI AGAINST HUMAN CERVICAL CANCER

Objective To obtain the gene of murine Single chain Fv fragment （ScFv） against haman cervical cancer and to express it in E. coli. Methods The variable region gene fragments of the heavy and light chains, which were amplified respectively using recombinant DNA techniques from CsA125 hybridama cells, were spliced together through a flexible linker to ScFv against human cervical cancer. The ScFv genes were then cloned into expression vector pCANTAB 5E and expressed in E. coli HB2151 and TG1 respectively. The soluble ScFv were characterized by SDSPAGE and Western blot. The antigen-binding activities of the soluble and phage displayed ScFv were assayed by ELISA and cell immunohistochemical analysis. Results The expressed ScFv antibodies were soluble and phage displayed. soluble ScFv secreted and expressed in E. coli HB2151 induced by IPTG were confirmed with SDS-PAGE, Western blot and ELISA. The specific binding capacity of the soluble and phage displayed ScFv to the surface associated antigen of human cervical cancer cell line was further confirmed with immunohistochemical studies. Conclusion The soluble and phage displayed ScFv expressed in E. coll against haman cervical cancer showed high, specific affinity for the cervical cancer cell line surface associated antigen.     

动荷载作用下风积土路基振陷变形

动荷载作用引起路基震(振)陷变形,是高速铁路路基抗震设计中必须考虑的问题之一.以哈大高铁苏家屯某试验段风积土为研究对象,通过大量室内动三轴试验,获得动应力幅值、含水率等条件下,风积土的动变形特性.并利用Matlab软件对试验数据进行处理,建立了考虑动应力、含水率两种因素条件的振陷变形模型.应用有限元分析软件ADINA建...     

商合杭铁路芜湖长江公铁大桥主桥钢梁架设关键技术

商合杭铁路芜湖长江公铁大桥主桥为主跨588m的双塔双索面高低塔箱桁组合梁斜拉桥,该桥钢主梁采用箱桁组合结构。主桥钢梁节段船运至桥位,在主墩墩旁搭设简易钢支架,采用1 000t浮吊吊装、拖拉滑移法架设主墩墩顶节段钢梁;拼装800t变幅式桅杆起重机后双悬臂架设钢梁至辅助墩;辅助墩墩顶钢梁采用800t浮吊吊装架设,桥面架梁吊机再悬臂架设钢梁至边墩,将边墩墩顶钢梁分层叠放后再依次用架梁吊机吊装就位;中跨合龙段利用无为侧架梁吊机提升,采取部分斜拉索索力调整、桥塔墩墩顶顶推纵移、温差调整等措施,实现了高精度、快速顺利合龙。     

新环保塑料材料用于新能源汽车内饰的探讨

近些年来,汽车在全世界范围保有量的不断飞速增长,是人类面临全球变暖,石油资源短缺,空气污染和水质污染等很多问题变得越来越严重,而基于这些问题的出现,各大车企相继开发出各种类型的电动新能源汽车,电动新能源汽车成为解决这些问题的最佳方案。而汽车随着消费者需求的变化,汽车内饰面料不仅要满足实用性、经济性、耐久性的要求,还应该向着人性化、舒适化、美观化、环保化等方向发展。针对这些新的功能需求,文章从新环保材料等方面进行了汽车内饰面料的设计与开发。     

钢桥面铺装环氧树脂沥青混合料性能研究

钢桥面铺装是钢桥建设中的难点,该文研究了环氧树脂沥青混合料应用于钢桥面的铺装技术,包括材料组成、集料级配、粘结层强度、混合料路用性能以及环氧树脂沥青混合料拌和和摊铺等方面的问题.研究表明:环氧树脂沥青混合料用于钢桥面铺装层,各方面性能都比较优越,是一种良好的钢桥面铺装材料.     

民用飞机起飞第2爬升段爬升梯度计算分析

通过建立飞机的动力学方程，利用性能工程师手册中的原始数据，编程计算起飞第2爬升段的爬升梯度大小，对其影响因素进行了分析，将计算结果与波音公司提供的相关曲线进行比对，表明计算结果准确可靠。