Analysis of chiral non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac binding with HSA

The protein binding of non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac with human serum albumin （HSA） was investigated using indirect chiral high performance liquid chromatography （HPLC） and ultrafiltration techniques. S-（-）-1-（1-naphthyl）- ethylamine （S-NEA） was utilized as chiral derivatization reagent and pre-column derivatization RP-HPLC method was established for the separation and assay of the three pairs of enantiomer. The method had good linear relationship over the investigated concentration range without interference. The average extraction efficiency was higher than 85% in different systems, and the intra-day and inter-day precisions were less than 15%. In serum albumin, the protein binding of etodolac enantiomers showed significant stereoselectivity that the affinity of S-enantiomer was stronger than R-enantiomer, and the stereoselectivity ratio reached 6.06; Flurbiprofen had only weak stereoselectivity in HSA, and ketoprofen had no stereoselectivity at all. Scatchard curves showed that all the three chiral drugs had two types of binding sites in HSA.     

Witen港口行业解决方案的持续演进

<正>2012年,鼎桥通信技术有限公司(以下简称鼎桥)基于4G TD-LTE(LTE:Long Term Evolution,长期演进)无线通信技术,开发了宽带多媒体数字集群系统Witen港口行业解决方案,并于2012年在中国烟台港首次应用。鼎桥着眼于港口行业的整体发展,聚焦于港口码头生产作业对调度指挥通信系统的实际需求变     

脾大脾脏巨噬细胞在门脉高压症脾亢发生中的作用

目的　探讨脾大、脾脏巨噬细胞 (MΦ)在门脉高压症 (PHT)脾亢发生中的作用。方法　采用免疫组化和透射电镜等方法 ,对比观察PHT脾脏MΦ 内ACP含量 ,MΦ 超微结构及MΦ 吞噬破坏血细胞数量的变化。并对PHT患者的脾指数、MΦ 内ACP含量及外周血细胞计数三者之间作相关分析。结果　同正常脾脏相比 ,PHT脾脏MΦ 内ACP含量增多 ,MΦ 表面伪足增多、增长 ,胞内溶酶体数量增多 ,MΦ 吞噬破坏红细胞、血小板的数量明显增多。PHT患者的脾指数和MΦ 内ACP含量与外周血细胞计数成反比 ,脾指数和MΦ 内ACP含量成正比。结论　脾大、脾脏MΦ 在PHT脾亢的发生中起着重要作用。     

Implementation of Heat Treatment Process Simulation with Object-Oriented Method

IntroductionDuringheatingorcoolingprocess,metallicmaterialsundergotheevoluhonsoftemPeraturefield,phasecompositionsandstrCssfield.ThereexistseveralinteaCtionsl1-4](couPlingeffeCts)betWeenthesephenomena.TodescribeallthesephysicalprocessescorrecUyandefficientiy,thefiniteelementmethodisoftenadoPtedll'5'6].Asitiswellknown,conventionalfiniteelementanalysisprogramsarecomplicatCdanderror-Prone.ComPlexcontrolanddatastructuresassociatedwithsoftwaretendtOobscurefundamerealintenhons,maldngdevelopmen…     

基于Cortex-M3嵌入式控制器船舶吹气式液位检测装置研究

船舶压载水液位检测系统是船舶自动化的重要组成部分,直接关系到船舶在海上航行的安全性和可靠性.本项目在对现有嵌入式系统和船舶压载舱吹气液位检测方式进行深入研究的基础上,提出了基于嵌入式Cortex-M3控制器船舶吹气液位检测装置的研制方法,并给出电路模块的硬件和软件设计思路.     

Fluorescent Property of Triethylene-glycol Di （ 7-（ N, N＇-diethylamino） ）-Coumarin- 3-Carboxylate

Coumarines have been recently paid much attention for their physiological activities and fluorescent properties. They have outstanding optical properties, including high photostability, extended spectral range, high quantum yield of photoluminescence.     

海绵城市径流控制模型研究

通过模型可以精细化地计算海绵城市的核心指标——年径流总量控制率，但建立模型时需要关注其参数、边界，并对概化以及率定进行相应的规范化。为此，在建模过程中，对降雨序列长短、步长影响以及模型概化过程中的管网考虑进行了分析，同时比较了渗透速度、不透水率、硬化面积收集率、蒸发量的不同设置方式等建模敏感参数，并对率定方式进行了初步探索。所归纳出的结论可为其他海绵城市径流模型搭建提供借鉴。     

汽车如何牵引和被牵引

自己的车被牵引或牵引别人的车虽不是常事,但也是不可避免的事.因在行车中难免会因机械故障或操作不当,造成车辆的损坏而难以行驶.一旦遇到此情况,在那上不巴村下不挨店的公路上,因一时无法修复,就只得靠别的车将坏车牵引到有修理店的地方去.