城市轻轨车辆转向架方案的选择

本文根据城市轻轨车辆用转向架的运用条件和技术要求,结合我国的技术水平和传统特点,对城市轻轨车辆转向架结构设计中应考虑的问题进行了分析,认为在进行轻轨车辆转向架设计时,应采用空气弹簧、盘形制动,弹性车轮、磨耗形踏面、焊接构架等先进技术.     

基于DVS 1612标准的焊接构架疲劳强度数值解法研究

针对应用DVS 1612标准评估焊接构架的疲劳强度时,应力分量提取繁琐的问题。利用空间向量运算的性质,提出在焊缝评估点处建立局部坐标系的数值解法,进而将全局坐标系下的应力分量转化为局部应力分量。利用某地铁车辆焊接构架,验证该方法的可行性。结果表明,在有限元模型能够真实反映焊接结构的条件下,2种方案的计算精度都可以满足工程实际需要。采用方案一对曲线型焊缝进行疲劳强度评估时,应控制评估点处的尺寸比不超过0.16以保证计算精度,方案二计算精度不受尺寸比影响。     

钢管混凝土系杆拱桥的施工监控

结合扬州市233省道北澄子河大桥的工程实例,对钢管混凝土系杆拱桥施工监控的内容和方法进行了针对性阐述,以供同类型桥梁参考。     

DNMT1、β-catenin和P-GSK-3β在结肠癌组织中的表达

目的 探讨DNA甲基转移酶1(DNMT1)、β-连环素(β-catenin)和磷酸化糖原合成酶激酶(P-GSK-3β)在结肠癌中的表达及其与肿瘤分化程度和淋巴结转移等的关系.方法 采用荧光定量PCR检测62例原发性结肠癌患者癌组织及21例正常大肠黏膜组织中DNMT1、β-catenin和GSK的表达,采用Westernblot方法验证蛋白的表达情况.结果 结肠癌组织中DNMT1、β-catenin和P-GSK-3β表达程度显著高于正常组织.DNMT1在低分化程度的癌组织间相对含量高,β-catenin在肿瘤的不同分化程度及有无淋巴结转移的癌组织间相对含量的差异有统计学意义,而P-GSK-3β的组间比较无显著性差异.结论 DNMT1、β-catenin和P-GSK-3β的活性改变与结肠癌有一定的相关性.     

�۽�ͨϵͳһ�廯

指出了一体化交通系统是与智能交通系统并行的世界性的发展趋势，论述了交通系统一体化的必要性。提出了从三个方面理解一体化交通系统的内涵:交通系统的一体化是对交通系统资源的整体优化，交通系统的一体化是对交通需求的统一管理，交通系统的一体化体现在交通运输的全过程。指出了交通系统一体化的外延。重点讨论了两个问题:一体化交通系统与智能交通系统的关系问题，ITS与交通工程的关系问题。提出了把非技术范畴的因素从ITS中分离开来的观点。     

THE STUDY OF MICROSATELLITES ALTERATION IN DIAGNOSES OF BLADDER CANCER

Objective To investigate the possibility of microsatellite alteration （MA） in diagnosis of bladder cancer of Chinese people, and find the better panel which will be used in clinic. Methods A total of 6 and 10 microsatellite markers were chosen, PCR-SSLP silver staining assay was performed in 31 and 32 bladder cancers tissue, exfoliate cells in urine and 10, 15 non-bladder cancers exfoliate cells in urine, respectively. Results MA （＋） was found in 28 out of 31, 30 out of 32 bladder cancers, and the sensitivity was 90. 3%, 93.7% respectively. The MA of urine sediment of 25 non-bladder cancers was negative, and the specificity was 100%. The cytology was carried out among 19 out of 31, 20 out of 32 bladder cancers at the same time, 2 cases （10.3%） and 3 cases （15%） were found cancer positive, and the sensitivity is significantly lower than that by the analysis of MA in exfoliated cells. Conclusion MA was not associated with grade and stage of the bladder cancer. MA assay is a sensitive and effective method for the early detection of bladder cancer and post-operation surveillance.     

REAL-TIME DETECTION OF SURVIVIN mRNA EXPRESSION IN CERVICAL CANCER CELL LINES USING MOLECULAR BEACON IMAGING

Objective To detect the expression of survivin mRNA in cervical cancer cell lines using molecular beacon imaging technology. Methods Human cervical cancer cells （HeLa and SiHa） and human fetal lung fibroblast HFL-I were cultured in vitro. After adding 100 nmol/L survivin mRNA molecular beacon, the fluorescent signals were observed under fluorescent microscope. The expressions of survivin in cervical cancer cells and HFL-I cell were examined by immunocytochemical streptravidin-biothin peroxidase （SP） assay at the same time. Results Two kinds of survivin mRNA molecular beacon, with different color fluorescence, had strong fluorescent signal in cervical cancer cell lines, and the signal in SiHa cell line was stronger, but these signals were not found in HFL-I ; Immunocytochemical staining of positive survivin was located in the cytoplasm of cervical cancer cell lines HeLa and SiHa, whereas, no expression of survivin was detected in HFL-I cell line. Conclusion The technology of molecular beacon imaging can be used to detect the expression of survivin mRNA in viable cells successfully, and may provide a new approach to the diagnosis of early stage cervical cancer and the following-up in the clinic.     

一种改良式质粒DNA提取方法的建立与应用

目的建立一种稳定、可靠的质粒DNA提取改良方法,探讨其在分子生物学实验研究中的应用价值。方法将含有目的质粒的菌株扩增后,运用改良后的碱裂解法进行质粒DNA小量提取,微量核酸仪测定提取质粒DNA的产量及纯度;采用琼脂糖凝胶电泳、内切酶酶切及质粒转染真核细胞鉴定提取质粒DNA的质量及转染效率。结果改良式质粒提取方法获得质粒DNA产量为3.2 mg/L菌液,A260/280=1.91;内切酶酶切完全;质粒DNA以超螺旋结构为主;真核细胞转染效率为20%左右。结论改良式质粒DNA抽提方法操作简单、实用,获得质粒DNA产量多、质量高,能达到分子生物学常规实验的要求。     

基于MFCM的改进聚类算法及其在交通中的应用

在交通流状态模糊化的过程中,对已有的交通模糊控制研究引入了太多的主观因素.为了解决这个问题,提出了一种基于MFCM算法的分级递减聚类算法,利用MFCM算法寻找类中心,再自适应确定该类中心的隶属度阈值,将聚类进行分级处理,实现未知类别数数据集的聚类.将改进算法应用到交通流状态聚类中,可以更科学地确定交通流状态的聚类数和各类模糊隶属度函数的结构等,最后,通过算例,说明了该算法对于未知聚类数及服从高斯分布的数据集具有聚类效果好、收敛速度快的特点.