Construction of eukaryotic recombinant vector of renalase and its expression as a eukaryotic protein

Renalase is a secreted amine oxidase that metabolizes catecholamines. It has been proposed to modulate blood pressure and heart rate and its downregulation might result in hypertension. Despite its potential relevance for human health, the biochemical characterization of renalase is still scarce. The aim of this study is to synthesize the human renalase eukaryotic protein by genetic engineering. The human renalase gene was amplified by polymerase chian reaction (PCR). After digestion by BamH I and Xho I enzymes, the DNA fragments were cloned into the transfer vector, pFastBacHTb-Fc, to generate the pFastBacHTb-renalase expression vector. The ligation products were transformed into E. coli DH10Bac to obtain recombinant transposon rBacmid-renalase. The recombinant transposon was further transferred into insect high-V cells, and the recombinant human renalase eukaryotic protein was expressed successfully.     

Numeric-based XML labeling schema by generalized dynamic method

Most efficient indeces and query techniques over XML (extensible markup language) data are based on a certain labeling scheme, which can quickly determine ancestor-descendant and parent-child relationship between two nodes. The current basic labeling schemes such as containment scheme and prefix scheme cannot avoid relabeling when XML documents are updated. After analyzing the essence of existing dynamic XML labels such as compact dynamic binary string (CDBS) and vector encoding, this paper gives a common unifying framework for the numeric-based generalized dynamic label, which can be implemented into a variety of dynamic labels according to the different user-defined value comparison methods. This paper also proposes a novel dynamic labeling scheme called radical sign label. Extensive experiments show that the radical sign label performs well for the initialization, insertion and query operations, and especially for skewed insertion where the storage cost of the radical sign label is better than that of former methods.     

Nonlinear feedforward and feedback control design for autonomous underwater glider

Underwater gliders are highly efficient, buoyancy-driven, and winged autonomous underwater vehicles. Their dynamics are multivariable nonlinear systems with unstable internal dynamics and thus their motion control is a significant challenge. To improve the inherent efficiency and enhance the behavior of the underwater glider over a wide operating regime, a nonlinear feedforward and feedback controller was developed. The nonlinear feedforward control design is based on a new stable inversion technique which determines a causal and bounded solution for the unstable internal dynamics. The feedback control law was designed by a quadratic optimal control method. Simulation results show that the derived control system is able to deal with nonminimum phase system and successfully achieves the tracking of planned output trajectories from initial to final conditions. Furthermore, the control effort is very low, which means the glider with limited power storage has longer range and higher endurance.     

Numerical analysis of material properties in deformation of near hemispherical shells

In this paper, the effects of hardening exponent, yield strength and elastic modulus on the deformability of near hemispherical shells are investigated by means of finite element method and orthogonal experiment design. The largest eccentric angle during the deformation process and thickness reduction after the deformation are introduced to estimate the deformability quantitatively according to the deformation characteristics of near hemispherical shells. The results indicate that the hardening exponent is the most influential parameter, followed by elastic modulus and yield strength. The shell exhibits good deformability when the hardening exponent and elastic modulus are in the range of 0.1–0.125 and 70–108 GPa, respectively.     

Dynamic investigation on floating raft with elastic limiters

In this paper, we consider a floating raft isolation system with elastic limiters. The limiters might be “contact” or “no contact”, thus forming a unilateral system generating possible contacts. In order to avoid the large displacement of the floating raft under shock, limiters might be adopted but good limiters design requires the values of the gaps. Based on the contact dynamics, the multi-degree-freedom dynamic model of floating raft isolation system with elastic limiters is established. The artificial neural network has been developed to dingtinguish the contact state of elastic limiters at each step. The example analysis shows that the algorithm of neural network for contact can shorten the time of caclaution. From the example analysis, we get some interesting results that may be useful to the ship engineering.     

Upper limb musculo-skeletal model for biomechanical investigation of elbow flexion movement

A biomechanical musculo-skeletal model of upper limb is presented in this paper, which can provide accurate representations of muscles and joints, and capture important interactions between joints. The upper limb model is made up of seven segments: ribs, sternum, clavicle, scapular, humerus, radius and ulna, considered as a single rigid body respectively and includes 22 muscles. The individual muscle forces can be calculated by using an electromyography (EMG) assisted method, which is verified by comparing the simulation results with other researches of an elbow flexion motion. These comparisons show that the muscle forces and the estimated joint moment match well with previous literatures.     

Slam events of high-speed catamarans in irregular waves

Slam events experienced by high-speed catamarans in irregular waves were characterised through experiments using a hydroelastic segmented model. The model was designed to represent the dynamic behaviour of the full-scale Incat 112 m vessel and to allow the measurement of the slam load on the centrebow and wet deck. It was tested in irregular head seas at two speeds relating to Froude numbers of 0.32 and 0.60. Nearly 300 slams were identified in the test data and analysed with respect to kinematic parameters. Slams were found to have a large range of magnitudes, with the largest equivalent to 1785 tonnes full scale (approximately 70% of vessel displacement); however, the majority of events were of relatively low severity. Differences in slam characteristics were found for the two model speeds tested; slams at the slower speed generally occurred further forward on the hull, prior to the wave crest and with a bow down pitch angle. Immersion of the centrebow to the two-dimensional filling height of the cross-section between the centrebow and demihulls is shown to be a better indicator of slam occurrence than immersion to the top of the archway.     

Dynamic motions of planing vessels in head seas

The dynamic response of planing vessels in regular head seas is investigated numerically. Nonlinear time domain simulations were performed using a 2D + t theory (two-dimensional plus time dependent theory). A prismatic hull form was assumed. We employed a two-dimensional (2D) boundary element method to solve the initial boundary value problems in 2D cross planes, in which nonlinear free-surface conditions and exact body boundary conditions were satisfied. At each time step, the total force and moment on the hull could be obtained by using the sectional forces calculated in those 2D planes. Heave and pitch motions were then acquired by solving the equations for those motions. The calculated heave and pitch responses were compared with the experiments by Fridsma (A systematic study of the rough-water performance of planing boats. Davidson Laboratory Report R-1275, 1969) for two different Froude numbers. Three-dimensional (3D) corrections at the transom stern were applied to show the influence of the 3D effect at the stern on the numerical results. Ship motions were affected by the 3D corrections, especially near the resonance frequency, while the phase angles were slightly affected and the acceleration peaks at the bow near the resonance frequency were sensitive to the 3D corrections. Other error sources in the theoretical results are also mentioned.     

Multi-fidelity optimization of a high-speed foil-assisted semi-planing catamaran for low wake

The wakes of high-speed passenger-only ferries that operated through Rich Passage, on the Seattle-Bremerton ferry route, caused beach erosion and damage to habitat. A task was initiated to design a low-wake high-speed vessel using multi-fidelity CFD based design optimization by using low-fidelity potential flow solvers for initial global design optimization and by using URANS solvers for high-fidelity tuning of the optimized design. This simulation based design process involved a close collaboration between ship designers, and hydrodynamics and CFD specialists, whose collective expertise guided the evolution of the design based on both hydrodynamic and structural aspects. The initial hull shape optimization using potential flow code was carried out by blending three different initial concepts provided by the designers. Subsequently, URANS was used to evaluate the potential flow optimized hull and to further optimize the hull configuration parameters, namely, the centre-of-gravity, demihull spacing, foil location, foil angle and slenderness ratio at different displacement conditions. The URANS based configuration optimization also took into account the far field wakes’ energy spectrum with an objective of reducing the energetic, low frequency far field wakes which are associated with beach flattening on the mixed sand and gravel beaches. Calculation of the far field wake using URANS would require an unfeasibly large domain size; therefore, a Havelock code with a source distribution matching the URANS calculated near field wave elevation was used to propagate the wakes into the far field. The end result of the optimization was a design with significantly reduced far field wake, which is currently being built for experimental testing.     

The effect of sloshing on the sway motions of 2D rectangular cylinders in regular waves

In this paper, we investigated the effect of sloshing on the sway motions of two-dimensional rectangular cylinders in regular waves, bearing in mind possible applications for LNG-FPSO and LNG-FSRU. First, we carried out experiments for two models with different drafts, or the same draft but different filling ratios, in which the models were firmly connected to each other. The sway motion was measured with a noncontact video camera. This is an extension of Rognebakke and Faltinsen’s work for a single model (J Ship Res 47(3):208–221, 2003). It was found that the sway motion became small when the incident wave frequency was close to the lowest natural frequency of each model. The sway motion greatly increased when the wave frequency was higher than this frequency. The measured data were compared with numerical results obtained by a single-dominant multi-modal method; relatively good agreement was noted. However, the numerical results deviated from the experimental results near the lowest natural frequency of the smaller model, which was believed to be due to overturning waves, as observed during the experiment. Since this is out of the valid range for the single-dominant multi-modal method, other, more appropriate, methods such as the multi-dominant modal method must be applied instead.