Induction of apoptosis and inhibition of proliferation of C6 glioma cells in vitro by tamoxifen

Objective To investigate the anti-tumor effect and mechanism of tamoxifen on rat C6 glioma cells. Methods C6 cells were cultured in Dulbecco＇s modified Eagle＇s medium （DMEM） with 3% fetal calf serum （FCS）, and treated with tamoxifen of different concentrations, i.e. group A （1.25 μmol/L）, group B （2.50 μmol/L）, group C （5. 00 μmol/L）, group D （10. 00 μmol/L）, group E （20. 00 μmol/L） and control group （0. 00 μmol/L）. Morphological changes, MTT assay and 5-bromo-2＇-deoxyuriding labeling ratio were assessed. Apoptosis was observed by flow cytometry. Results C6 cells treated with different doses of tamoxifen for 24, 48, and 72 hours became irregular in shape, while cells treated with vehicle grew normally. MTT assay showed that tamoxifen did not suppress C6 cell growth until 72 hours after treatment. Seventy-two hours after treatment, there were significant differences in cell viable rate between group A versus groups C, D and E; so did group B versus group D as well as group E （P〈 0.05 ）. BrdU incorporation assay indicated significant difference of BrdU labbled index （BrdU LI） among groups A, C, E and control group 48 hoers after treatment （P〈0.05）. And the BrdU LI decreased with the increased concentration of tamoxifen. Flow cytometry （FCM） showed significant difference between treated group and control group at 24, 48, and 72 hours after treatment （P〈0.05）. Conclusion Tamoxifen significantly suppresses the growth of C6 glioma cells in a time- and dose-dependent manner. The mechanism of tamoxifen suppressing C6 glioma cells may be inhibiting proliferation and inducing apoptosis. Therefore, tamoxifen can be a candidate as a chemotherapy agent for glioma.     

自密实混凝土在钢管系杆拱桥中的应用

结合自密实混凝土在钢管混凝土系杆拱中的应用实践,对自密实混凝土的制备原理、配合比设计方法和原材料选择原则以及施工工艺控制关键要素等进行了阐述。     

β淀粉样前体蛋白裂解酶特异性小干扰RNA真核表达载体的构建和鉴定

目的构建β淀粉样前体蛋白裂解酶(BACE)特异性小干扰RNA真核表达载体。方法用PCR扩增BACE特异性小干扰RNA,转入带有增强型绿荧光蛋白(EGFP)和启动子U6的pUC19质粒,再将重组基因片段导入逆转录病毒真核表达载体pLXSN中,通过限制性酶切对该重组表达载体进行鉴定。结果成功构建了BACE真核表达载体pLXSN/EGFP-U6-siBACE。结论pLXSN/EGFP-U6-siBACE载体的成功构建对进一步利用基因干扰技术治疗阿尔茨海默病的研究奠定了重要的基础。     

β-淀粉样蛋白诱导大鼠海马3100β表达的作用及机制

目的探讨β-淀粉样蛋白(Ap)对S100β表达的影响及作用机制。方法采用Aβ25-35和IL-1ra，选择大鼠海马CA1区进行定位注射，通过行为学测试、刚果红染色和免疫组化技术结合图像分析的方法，对不同时间大鼠的学习记忆、淀粉样沉积、GFAP和海马CA1区S100β表达的变化进行观测。结果 Aβ25-35注射后，大鼠的学习记忆能力明显下降；海马CAl区出现AB沉积，并有大量GFAP阳性胶质细胞包绕；实验组$100~阳性细胞数目在3、7、21d较对照组均有明显增多，细胞平均面积只在21d才有明显增大。脑内注射IL-1ra后3、7d，S100β阳性细胞数目明显低于同组的AB大鼠，而高于对照组。结论Aβ25-35脑内注射可建立具有类似阿尔茨海默病的局部病理改变和学习记忆损害的AD动物模型；Aβ25-35可上调大鼠海马CA1区S100β的表达，实验早期以S100β阳性细胞的数目增加为主，后期表现为细胞体积的增大；IL-1ra脑内注射可明显降低Aβ25-35上调S100β表达的作用，其机制是阻断由Aβ25—35激活的小胶质细胞释放的IL-1对星形胶质细胞的活化作用。     

κ阿片受体激动剂U50,488H的利尿作用机制

目的观察κ阿片受体激动剂U50,488H对大鼠的利尿作用并探讨其作用机制。方法用生理学整体实验技术观察U50,488H对尿量的影响;分离大鼠肾动脉,用血管环方法测定肾血管张力的变化;应用放射免疫分析方法观察其对血浆中抗利尿激素(ADH)、血管紧张素Ⅱ(AngⅡ)和心房钠尿肽(ANP)的影响。结果静脉注射U50,488H可显著增加大鼠的尿量,并且使大鼠血浆ADH水平显著性下降;在离体水平,U50,488H可以剂量依赖性地舒张大鼠的肾动脉。以上效应均可被κ阿片受体选择性阻断剂(nor-BNI)所阻断。结论U50,488H通过激动κ阿片受体使大鼠的尿量增加,舒张肾动脉和降低ADH的分泌是其利尿的主要机制。     

β-分泌酶底物肽反转录病毒载体及其包装细胞株的构建

目的用pLXSN反转录病毒载体构建介导β-分泌酶底物肽(BACEsp)基因表达的pLXSN-BACEsp反转录病毒载体,并建立高效、稳定表达BACEsp的包装细胞株。方法根据BACEsp基因序列设计引物,PCR合成的BAC-Esp片段和pLXSN载体用EcoRⅠ、BamHⅠ双酶切后连接,构建成表达BACEsp的pLXSN-BACEsp反转录病毒载体。用脂质体介导转染PT67包装细胞,经抗性筛选后,挑单克隆扩大培养,用NIH3T3细胞测定病毒滴度,筛选出高效产毒细胞株。结果经酶切、连接后构建成的质粒称为pLXSN-BACEsp,经测序证实构建成功,无任何碱基突变。经脂质体介导转染包装细胞、抗性筛选和测定病毒滴度,筛选出具有高病毒滴度的细胞集落,扩大培养后建立BAC-Esp高效表达的包装细胞株。结论成功构建了能表达BACEsp的pLXSN-BACEsp反转录病毒载体,并建立了稳定、高效地产生有活性的BACEsp产毒包装细胞株。     

基于AUTOSAR的SOME IP通信及其多核应用的实现

文章介绍了基于AUTOSAR标准的多核操作系统,以及SOME IP通信和SOME IP在多核操作系统上的实现方式。阐述了AUTOSAR多核操作系统的分层架构、多核ECU的启动流程、核间交互的方式以及SOME IP通信的基本原理及其基本应用。最后,使用Davinci Configurator和Developer开发工具,在MPC5748G 三核MCU上验证了SOME IP多核应用的可行性。     

Calibration efficiency improvement of rule-based energy management system for a plug-in hybrid electric vehicle

This paper presents a calibration method of a rule-based energy management strategy designed for a plug-in hybrid electric vehicle, which aims to find the optimal set of control parameters to compromise within the conflicting calibration requirements (e.g. emissions and economy). A comprehensive evaluating indicator covering emissions and economy performance is constructed by the method of radar chart. Moreover, a radial basis functions (RBFs) neural network model is proposed to establish a precise model within the control parameters and the comprehensive evaluation indicator. The best set of control parameters under offline calibration is gained by the multi-island genetic algorithm. Finally, the offline calibration results are compared with the experimental results using a chassis dynamometer. The comparison results validate the effectiveness of the proposed offline calibrating approach, which is based on the radar chart method and the RBF neural network model on vehicle performance improvement and calibrating efficiency.     

考虑个体异质性的汽车分时租赁选择行为

传统交通行为模型缺乏对个体异质性的考虑，导致其对真实选择行为的解释可能存在差距. 为了研究个体异质性对出行选择行为的影响，首先，分别构建了基于混合logit的选择模型以及基于潜在类别条件logit的选择模型；其次，使用正交设计法生成意愿调查问卷，在成都市开展新能源汽车分时租赁的出行选择实证调查；最后，利用极大似然模拟，采用Halton序列抽样对混合logit模型进行标定；采用回归分析对潜在类别条件logit模型进行求解. 结果表明:步行时间、候车时间、车内时间以及出行费用是影响出行方式选择的关键因素，两种模型均反映出个体异质性对出行者选择行为有着显著影响；潜在类别条件logit模型的拟合优度为0.143，优于混合logit模型的0.139，前者命中率为77.85%，也高于后者的61.28%；潜在类别条件logit模型将出行者划分为3个类别，区分度为0.908 4；类别1群体对出行费用最为敏感，对候车时间不敏感；类别2群体对步行时间和候车时间更加敏感，对费用敏感程度较低；类别3群体对时间和费用的敏感程度介于类别1和类别2之间.      

A dynamic approach to road freight flows modeling in Spain

This paper presents an innovative approach to analyzing road vehicle freight traffic that uses a dynamic panel data specification derived from a gravity model. This dynamic approach, which has recently been employed in international goods trade models in lieu of the traditional static specification, is applied to the case of Spain using data for the countrys 15 NUTS-3 regions between 1999 and 2009. Using the system general method of moments approach, we obtained significant evidence that the flow of vehicles carrying commodities by road has a strong persistence effect when controlling for unobserved heterogeneity. We also found that the quality of road transport infrastructure has a significant impact on vehicle trips. According to our findings, we suggest that this type of specification be employed in distribution models in which fixed effects and lags of the dependent variable are included to account for unobserved heterogeneity and persistence effects, respectively.