苦参碱对人卵巢癌细胞增殖的影响及其机制

目的 研究苦参碱(matrine)体外培养的人卵巢癌细胞OV2008增殖的影响及相关机制.方法 采用四甲基偶氮唑蓝(MTT)比色法检测不同浓度(0、0.125、0.25、0.5、1.0,2.0、4.0 mg/mL)的苦参碱在不同的时间点(24、48、72 h)对OV2008细胞增殖的影响;采用流式细胞术(FCM)检测不同浓度的苦参碱作用于OV2008细胞48 h后细胞周期分布、凋亡率及Fas、Bcl-2蛋白的表达水平.结果 苦参碱对OV2008细胞的增殖具有明显的抑制作用,与对照组相比具有显著性差异(P<0.05或P<0.01),并呈现时间和剂量依赖性;细胞周期分析显示,随着苦参碱浓度的增高,G0/G1期细胞比例逐渐增加,S期细胞比例减小,G2/M期细胞比例无明显变化;苦参碱能够诱导细胞凋亡,且凋亡率随着苦参碱浓度的增高而升高(P<0.01);苦参碱能够上调Fas蛋白的表达(P<0.05或P<0.01),下调Bcl-2蛋白的表达(P<0.01).结论 苦参碱可显著抑制OV2008细胞的增殖,且抑制作用晕剂量和时间依赖性.苦参碱对OV2008细胞的增殖抑制作用可能与其诱导细胞周期阻滞及凋亡有关,而苦参碱诱导凋亡可能与其上调Fas蛋白表达、下调Bcl-2蛋白表达有关.     

Dynamics of inorganic nutrient species in the Bohai seawaters

Within the frame of a Sino-German Joint Research Program, two cruises of “R/V Dong Fang Hong 2” were carried out in September–October 1998 and April–May 1999, respectively, to understand the dynamics of nutrients in the Bohai. Nutrient species (NO₃⁻, NO₂⁻, NH₄⁺, PO₄³⁻ and SiO₃²⁻) are determined colorimetrically on board for five anchor and 30 grid stations. In situ incubation experiments are performed to determine planktonic nutrient uptake and benthic exchange flux. Nutrient concentrations display short-term variability and seasonal change in the Bohai, with higher levels in shallow coastal waters than in the Central Bohai. The influence of riverine discharge on nutrient levels can be seen from salinity isopleths, nutrient distribution and species ratios. Near-bottom (nb) waters have similar nutrient concentrations as to the surface waters in the Central Bohai, whereas stratification takes place in the Bohai Strait and North Yellow Sea. In situ incubation experiments provide evidence that the uptake ratio (i.e. N, P) by phytoplankton is proportional to the ratios among nutrient species in ambient waters. Based on the data of this study and previously publications, a preliminary estimate of nutrient budgets via riverine input and atmospheric deposition is established. The results indicate that atmospheric deposition gains importance over rivers in delivering nutrients into the Bohai and sustain the new production, following recent decrease in riverine inflow caused by drought periods in North China and damming practices. A historical review of nutrient data indicates that concentrations of nitrogen increase and phosphorus and silica decrease in the Central Bohai over last 40 years. This potentially has an important influence on the health of ecosystem in Bohai (e.g. food web and community structure), though further study is needed to examine the scenario in more detail.     

Calculation of strain amplification matrix for strain invariant failure theory based on representative volume element models with periodical boundary condition

Strain invariant failure theory (SIFT) is a micro-mechanics-based failure theory for multi-scale failure analysis of composite materials originally proposed by Gosse and Christensen. In this paper, the approach for obtaining strain amplification matrix which is a key step for the execution of SIFT is improved by adopting representative volume element (RVE) finite element models considering periodical boundary condition, based on which more actual deformation mode is reflected. The deformation modes and strain data at the characteristic points of the centroid cell of multi-cell RVE model are analyzed and taken as a reference. It can be concluded that more reasonable deformation mode and relationship between the micro-mechanical and macro-mechanical strain states are obtained by employing the new model. Finally, numerical examples are provided to illustrate the determination of strain amplification factors within the RVEs considering periodical boundary condition at the characteristic points.     

在建盾构隧道突发管片破损病害成因分析及治理措施研究北大核心CSCD

盾构法广泛应用于我国城市轨道交通隧道的建设中,盾构管片的病害问题也越发受到重视。文章针对某地铁在建盾构隧道突发管片破损病害,绘制了管片破损病害展布图,分析了相关资料和检测数据,明确了病害的成因机理,制定并实施了相应治理措施。研究结果表明:管片背后大范围空洞导致围岩对隧道的约束不足,引起已成型隧道在盾构机反推力和扭矩、同步注浆浆液浮力、刀盘水土压力和扭矩等作用下发生类压杆弯扭失稳是导致该病害的主要原因;隧道变形监测数据表明"背后注浆填充+破损部位修复"两阶段治理措施取得了良好的治理效果;盾构隧道施工过程中,应严格管控同步注浆质量,防止隧道轴线偏移引起盾构管片发生开裂破损等病害。     

纤维增强复合材料拉伸破坏过程的Monte-Carlo模拟

基于剪切滞后模型,采用影响函数叠加方法,得到复合材料在多纤维断裂下的应力场,包含了基体和界面塑性变形的影响.考虑纤维强度的统计特性,结合Monte-Carlo方法模拟丫复合材料在拉伸载荷作用下的累计破坏过程.结果表明:复合材料拉伸强度的尺寸效应与纤维强度分布的统计性质以及微观损伤诱发的应力重分配有关.文中的研究为进一步发展基于破坏机理分析的复合材料在热/机械载荷作用下的疲劳寿命预测理论奠定了基础.     

松花江干流大顶子山航电枢纽工程库区蓄冰处理及枢纽导冰措施研究

通过实地调研、原型观测、现场实验等手段和方法对“水库冰冻对通航的影响问题及降低和消除影响措施已是目前急需解决的关键问题”进行了较系统的研究,提出了水库蓄冰处理及枢纽导冰措施方案,并在工程运行中得到验证,取得了预期的效果。     

The importance of oceanographic fronts to marine birds and mammals of the southern oceans

During the last 30 years, at-sea studies of seabirds and marine mammals in the oceans south of the Subtropical Front have described an association with major frontal areas. More recently, the advancement in microtechnology has allowed the tracking of individuals and investigations into how these marine predators actually use the frontal zones. In this review, we examine 1) the relative importance to apex predators of the different frontal zones in terms of spatial distribution and carbon flux; 2) the processes that determine their preferential use; and 3) how the mesoscale dynamics of frontal structures drive at-sea foraging strategies of these predators. We review published results from southern waters and place them in a broader context with respect to what has been learned about the importance of fronts in oceans farther north.Some fronts constitute important boundaries for seabird communities in southern waters. At a mesoscale the maximum values of seabird diversity and abundance correspond to the location of the main fronts. At-sea surveys show a strong curvilinear correlation between seabird abundance and sea surface temperatures. High mean species richness and diversity for whales and seabirds are consistently associated with the southern water mass boundary of the Antarctic Circumpolar Current, the Subtropical Front and the Subantarctic Front; in the case of the Polar Front mean seabird densities are more variable. At small-scales, variation in seabird occurrence has been directly related to the processes at fronts in a limited number of cases. A significant positive relation was found between some plankton feeding species and frontal temperature gradient–phytoplankton variables.Telemetric studies have revealed that several apex predators (penguins, albatrosses, seals) perform long, directed foraging trips either to the Subtropical front or Polar Front, depending on locality. Seabirds with low flight costs, such as albatrosses, are able to reach fronts at long distances from colonies, showing variable foraging strategies as a function of the distances involved. Diving birds such as King penguins, that travel at a higher cost and lower speed, rely on the predictable spatial distribution of mesopelagic fish found close to the Polar Front. They may use the currents associated with eddies as oceanographic cues in the active search for frontal zones. Once in these areas they dive preferentially in and below the depth of the thermocline where catches per unit effort are high. Elephant seals concentrate foraging activity principally inside or at the boundary of cyclonic eddies. These mesoscale features appear to offer exceptional productivity favourable for foraging by various diving top predators.The connection between biophysical parameters at fronts and predators is likely to be made through biological enhancement. Top predators appear to forage at locations where prey are advected by physical processes and others where prey are produced locally. Long-term research on at-sea distributions and demographic parameters of top predators are essential to assess the consequences of potential shift in front distributions in relation to global warming. Such environmental changes would add to the impact of fish extraction by the industrial fisheries on the southern food webs.     

On the numerical accuracy of the prediction of resistance coefficients in ship stern flow calculations

This article presents a study on the accuracy of the numerical determination of the friction and pressure resistance coefficients of ship hulls. The investigation was carried out for the KVLCC2 tanker at model- and full-scale Reynolds numbers. Gravity waves were neglected, i.e., we adopted the so-called double-model flow. Single-block grids with H–O topology were adopted for all the calculations. Three eddy viscosity models were employed: the one-equation eddy viscosity and the two-equation models proposed by Menter and the TNT version of the two-equation k-ω model. Verification exercises were performed in sets of nearly geometrically similar grids with different densities in the streamwise, normal, and girthwise directions. The friction and pressure resistance coefficients were calculated for different levels of the iterative error and for computational domains of different size. The results show that on the level of grid refinement used, it is possible to calculate the viscous resistance coefficients in H–O grids that do not match the ship contour with a numerical uncertainty of less than 1%. The differences between the predictions of different turbulence models were larger than the numerical uncertainty; however, these differences tended to decrease with increases in the Reynolds number. The pressure resistance was remarkably sensitive to domain size and far-field boundary conditions. Either a large domain or the application of a viscous–inviscid interaction procedure is needed for reliable results. This work was presented in part at the International Conference on Computational Methods in Marine Engineering—MARINE 2007, Barcelona, June 3–4, 2007.     

On the observability of the fortnightly cycle of the Tagus estuary turbid plume using MODIS ocean colour images

Using three years (2003 to 2005) of MODIS-Aqua normalized water-leaving radiance at 551 nm this paper shows a fortnightly cycle in the Tagus estuary turbid plume. The Tagus estuary is one of the largest estuaries of the west coast of Europe and is located in the most populated area of Portugal, including the capital Lisbon. The turbid plume has been detected by the backscattering characteristics of the surface waters in the vicinity of the estuary mouth. In fortnightly scales, the turbid plume has smaller dimensions during and after neap tides and higher dimensions during and after spring tides. This is most probably associated with the fortnightly spring–neap tidal cycle and the consequent increase in turbidity inside the estuary during spring tides. During the summer weak spring tides (tidal amplitude approximately 2.5 m) no turbid plume is observed for an entire fortnightly cycle. Outside the summer months, precipitation, river discharge and winds, were found to increase the turbid area, but the fortnightly cycle appears to be superimposed on the large time-scale variability, and present throughout the year.