基于下游大水位变率的下水式垂直升船机随动技术的设计与实现

下游航道水位变率大,快速适应水位变化的准确停位随动技术,对下水式升船机的传动控制很重要.本文介绍下水式垂直升船机随动控制技术.首先,对控制对象的特性进行分析,描述了系统的速度运行曲线,对位置控制功能进行了研究.然后,搭建试验平台对本控制方案的性能进行了测试,给出了测试的方法和试验结果.实验结果表明本控制系统具有定位精度高、响应速度快等优点,能够适应下游大水位变化率的下水式垂直升船机准确停位要求.     

新冠肺炎疫情对粤港澳大湾区港口的影响及对策建议

2020年新冠疫情的爆发对粤港澳大湾区港口的发展产生了深远的影响。基于目前的发展形势，预判新冠肺炎疫情对粤港澳大湾区港口发展的影响总体可控，后期港口发展将逐步回归正常，甚至超越疫情前水平。从科学开展疫情防控、加快智慧港口建设、完善集疏运体系、延伸港口服务功能等方面提出后疫情时代粤港澳大湾区港口的发展建议。     

港口现有模拟化视频监控系统的数字化改造

为了提高对港口设备的监控水平，秦皇岛港口对SOLAS模拟监控系统实施了数字化改造。介绍监控技术的发展现状、设备背景和改造思路，以及改造所需的设备和改造方法。采用模拟与数字化相混合的数字监控系统，高效、安全，满足了生产需求。     

A proposed vision and vehicle-to-infrastructure communication-based vehicle positioning approach

It is essential to obtain accurate location of vehicles for new applications of Intelligent Transportation Systems. To remedy the defects of present Global Positioning System and vehicle-to-infrastructure (V-I) positioning technology, a new positioning approach based on vision and V-I communication is proposed. This approach aims at lane-level positioning with lower cost than conventional ones. In this approach, the position of the vehicle is represented by its lateral position (the lane number) and longitudinal position (the distance from entrance of the road) in a course coordinate system along the road; the specific lane the vehicle is occupying (the lane number) can be judged using the information of lane lines detected by vision systems; then the distance to the vehicle is obtained by a Road Side Unit (RSU) during the V-I communication; and the longitudinal position is calculated. The error of the approach on typical operating conditions is analyzed, indicating that the new approach can achieve the accuracy of less than 0.31 m for straight road and 0.58 m for typical arc road with ultra-wideband communication and ranging technologies and rational arrangement of RSUs. The feasibility of this approach is presented.     

Approximate optimal AUTOSAR software components deploying approach for automotive E/E system

The AUTOSAR has been developed as the worldwide standard for automotive E/E software systems, making the electronic components of different suppliers to be employed universally. However, as the number of component-based applications in modern automotive embedded systems grows rapidly and the hardware topology becomes increasingly complex, deploying such large number of components in automotive distributed system in manual way is over-dependent on experience of engineers which in turn is time consuming. Furthermore, the resource limitation and scheduling analysis make the problems more complex for developers to find out an approximate optimal deploying approach in system integration. In this paper, we propose a novel method to deploy the AUTOSAR components onto ECUs with the following features. First, a clustering algorithm is designed for deploying components automatically within relatively low time complexity. Second, a fitness function is designed to balance the ECUs load. The goal of our approach is to minimize the communication cost over all the runnable entities while meeting all corresponding timing constraints and balancing all the ECUs load. The experiment results show that our approach is efficient and has well performance by comparing with other existing methods in specific and synthetic data set.     

城市地下道路分(合)流匝道通风阻力特性

为分析多点进出结构的城市地下道路空气流动特性,以长沙市营盘路湘江隧道为原型,通过现场实测、缩尺模型试验以及CFD软件数值模拟方法,对分(合)匝道通风阻力特性进行了研究,考察了雷诺数、风量比、分岔角度对分(合)流匝道阻力特性的影响规律。研究结果表明:1)主隧道与匝道风量比、主隧道与匝道夹角是影响分(合)流匝道局部阻力系数的关键因素;2)城市地下道路分(合)流匝道局部阻力变化特性,不宜简单套用通风管道的三通构件的参数;3)结合最小二乘法和Matlab软件对计算结果进行多因素回归分析,给出了基于长沙营盘路湘江隧道的分(合)流匝道主隧道和匝道的局部阻力系数关联式。研究结果可为复杂结构城市地下道路通风系统阻力特性分析及通风工程优化设计提供方法参考。     

泡沫沥青冷再生基层力学性能的试验研究

对现场泡沫沥青冷再生基层的力学性能进行了研究,主要内容有根据泡沫沥青的性能指标确定了泡沫沥青的制备条件;根据国内对基层强度的要求和国外的规程,进行了无侧限抗压强度试验和间接抗拉劈裂强度试验,确定了最佳泡沫沥青用量,评价了泡沫沥青冷再生基层的力学性能。     

Apoptin原核表达载体的构建及在大肠杆菌中的表达

目的构建Apoptin的原核表达载体,并制备抗原物质Apoptin融合蛋白。方法在获得Apoptin融合基因的基础上,成功构建了Apoptin的高效原核表达载体pET-28a( )-Apoptin,将该质粒转化至大肠杆菌E.coliBL21(DE3)受体菌中,以IPTG对其进行诱导表达,聚丙烯酰胺凝胶电泳分析目的蛋白。结果转化有Apoptin的原核表达载体pET-28a( )-Apoptin的大肠杆菌E.coliBL21(DE3)经IPTG诱导后,经SDS-PAGE分析,在相对分子质量约17 000的位置出现目的蛋白条带,大小与Apoptin融合蛋白一致。结论Apoptin原核表达载体pET-28a( )-Apoptin能够表达出Apoptin融合蛋白,为进一步的Apoptin研究和制备Apoptin抗体奠定了基础。     

幽门螺杆菌尿素通道蛋白基因UreI载体的构建、融合表达及纯化

目的构建幽门螺杆菌(H.pylori,Hp)尿素通道蛋白基因UreI的融合表达载体,并在E.coliBL21中表达,为进一步研究UreI的功能奠定基础。方法利用分子克隆技术以Hp DNA染色体为模板,扩增Hp UreI基因片段,将目的基因UreI与载体pET32a( )分别经kpnⅠ和HindⅢ双酶切,纯化,连接。筛选阳性重组载体,转化大肠杆菌BL21,以IPTG诱导表达pET32a( )/UreI融合蛋白。表达产物经Ni-NTA琼脂糖树脂纯化后,用SDS-PAGE及Western blot分析。结果经酶切、测序分析表明,插入的基因片段为Hp UreI编码基因,由585 bp组成,与GenBank相应菌株HP AM417 609序列完全一致,无碱基突变。经SDS-PAGE分析显示重组质粒pET32a( )/UreI在原核细胞中得到了高效融合表达,其重组融合蛋白分子量为130 ku,经Ni-NTA琼脂糖树脂纯化后,其纯度达95%以上,Western blot分析显示,该重组融合蛋白可被6-His鼠抗单克隆抗体所识别,具有良好的反应原性。结论成功地克隆和构建了原核表达载体pET32a( )/UreI,并在原核细胞中得到了高效融合表达。     

新时期高职院校党建工作存在的问题及对策

文章通过分析目前高职院校党建工作存在的发展不平衡、学生党员入党前后的形象不和谐、学生党员整体作用不明显等问题,提出了深入基层宣传、抓好学生党员再教育、推进党员责任区工作、发挥教师党员教书育人作用以及加强流动党员管理等对策。