PROPERTIES OF PROLIFERATION AND DIFFERENTIATION OF NEONATAL RAT RETINAL PROGENITOR CELLS IN VITRO

Objective To investigate the properties of proliferation and differentiation of neonatal rat retinal progenitor cells （RPCs） in vitro. Methods RPCs were isolated from neonatal SD rats neural retina and cultured in DMEM/F12 ＋ N2 with EGF and bFGF （suspension medium ） or 10 % FBS without EGF and bFGF （ differentiation medium）. The cells grew as suspended spheres or adherent monolayers, depending on different culture conditions. The neural stem cells or retinal progenitors, neurons, astrocytes, retinal ganglion cells, rod photoreceptors and the proliferating cells were evaluated with immunofluorescence analysis by Nestin or Pax6, Map2, GFAP, Thy-1, Rhodopsin and BrdU antibodies respectively. Results RPCs could propagate and differentiate in suspension or differentiation medium and express the markers of Nestin （92.86%） or Pax6 （86.75%）, Map2 （38.54%）, GFAP （20.93%）, Thy-1 （27. 66%） and Rhodopsin（13. 33%）in suspension medium; however, Nestin （60.27%）, Pax6 （52%）, Map2 （34.94%）, GFAP （38.17%）, Thy-1 （30.84%） and Rhodopsin （34.67%） in differentiation medium. 96.4 % of the population in the neurospheres was BrdU-positive cells. The cells could spontaneously adherent forming some subspheres and retinal specific cell types. Conclusion Neonatal rat RPCs possess the high degree of proliferation and can differentiate into neurons, astrocytes, retinal ganglion cells and rod photoreceptors in vitro. There are different proportions for RPCs to differentiate into specific cell types.     

严格伪压缩映像不动点和均衡问题的公共元的迭代算法

均衡问题的解在最优化理论及应用中具有十分重要的意义。物理学和经济学中的很多问题可归结为寻找平衡问题的解。有关非线性算子不动点的迭代构造问题及混合均衡问题的求解方法,许多学者进行了研究。利用杂交投影算法,用以逼近Hilbert空间中严格伪压缩映像不动点集与混合均衡问题解集的公共元,并证明其强收敛性。此算法的最终投影具有显示表达,因而更有利于应用。所得结果改进和推广了最近一些文献的相应结果。     

软件技术专业校企合作办学模式的探索与实践

高职院校办学必须立足于培养动手能力强,能迅速适应劳动岗位的高技能型IT人才。本文结合校企合作办学模式的背景和发展现状,从实训中心建设,师资队伍建设和教学内容改革等三个方面阐述软件技术专业校企合作办学模式的新思路。     

Improvement of the second order approximation of the smoothed particle hydrodynamics

The smoothed particle hydrodynamics (SPH), as a fully Lagrangian particle method, has been successfully applied to astrophysical problems and extended to elastic dynamics and computational fluid dynamics.High order derivatives have to be approximated when elastic dynamics problems are modeled. However, the approximation errors in SPH could lead to computational failure in the case that the order of derivative is high.A novel method was proposed in order to improve the accuracy of SPH method, which shows the relationship between the selected functions and their SPH approximations. The entire involved system was represented by a finite number of particles that carry individual mass and occupy individual space, and the integral interpolation was approximated by a summation interpolation. In addition, error comparison was made between SPH method with and without the present improvement.     

Control method of three-level neutral-point-clamped inverter based on voltage vector diagram partition

A new modulation approach was presented for the control of neutral-point (NP) voltage variation in the three-level NP-clamped voltage source inverter, and the average NP current model was established based on vector diagram partition. Thus, theory base was built for balancing control of NP potential. Theoretical analysis and experimental results indicate that the proposed method for NP balancing control vector synthesizing concept based can make the average NP current zero, and do not influence NP potential within every sample period. The effectiveness of proposed research approach was verified by simulative and experimental results.     

ISAR干扰仿真与效果定量评估研究

随着逆合成孔径雷达(ISAR)在军事领域得到越来越广泛的应用,对其实施有效的干扰及效果评估方法已成为研究热点.文中基于图像准则,提出运用图像均值、方差、等效视数和图像动态范围等图像评估度量指标,在不同干扰样式和不同干扰工作方式条件下,对ISAR压制干扰进行了定量的干扰效果评估,给出了供工程参考的有效干扰JSR门限值.仿真结果验证了评估的正确性、可行性和有效性.     

Histological changes following surgically-assisted rapid tooth movement through resistance reduction and distraction osteogenesis in dogs

Objective To investigate the histological changes of rapid tooth movement in dogs treated by resistance reduction and distraction osteogenesis, aiming to establish an animal model and further to reveal the remodeling mechanism of rapid tooth movement. Methods A total of 8 local hybrid dogs were selected as subjects for this study. The second pre-molar was extracted on both sides. The experimental side underwent alvelor surgery for resistance reduction and a home-made tooth-borne intraoral distraction device was installed for rapid tooth movement, while for the other side (control side) only tooth-borne intraoral distraction device was used for rapid tooth movement. The longest active force-delivery span was 2 weeks, followed by 6-week retention. The distance between the moved tooth and anchor unit was recorded weekly, and radiography was performed for each side before and after distraction. The surrounding tissues including periodontal ligament and alveolar bone were sectioned for histological analysis. Results The average distance of tooth movement was 3.55mm on the experimental side and 1.11mm on the control side. The rate of tooth movement was notably higher (P<0.01) and no significant apical root resorption was detected by X-ray on the experimental side. The active alvelor bone remodeling was found on the tension and pressure sides. However, there was no significant difference between the experimental side and the control side after the retention period. Conclusion The rate of orthodontic tooth movement can be accelerated through resistance reduction and periodontal distraction without any unfavorable effects but at minimal anchorage loss.     

爆炸活塞式高速开关的分析与设计

提出了一种爆炸活塞式高速开关,它是一种新型大容量高速电路开断装置.介绍了爆炸活塞式高速开关的工作原理,采用ANSYS热电耦合仿真进行了通流能力设计,计算了装置炸药量,用LS-DYNA仿真进行了爆炸气缸抗爆强度的设计,综合这3个步骤设计了380V/2 000A装置样机,并通过试验验证了样机的性能.试验中装置主载流体开断时间约116μs,动触头分离初始阶段平均速度约8.5 m/s.该装置与其他爆炸式高速开关相比,具有易更换,炸药安全性、稳定性高,炸药用量小等优点.     

广梧高速公路(马安至河口段)生态环境保护与恢复

在修筑高速公路过程中破坏自然植被的现象极为普遍,若不及时对裸露区域加以覆盖,则会造成水土流失的严重后果。针对广梧高速公路(马安至河口段)绿化恢复及生态保护工作,创建一条“绿色通道,环保之路”,结合笔者多年的工作经验,介绍本项目所采用的常规绿化技术和创新生态恢复技术相结合的方法。     

模糊决策方法定量评价道路安全性的尝试

运用安全系统工程模糊决策方法．分析天津市东丽区驯海路的不安全因素，建立双层多因素集．通过线性代数运算评价，计算出驯海路定量安全性，从而得出道路是否需要整改的结论，并提出改进措施。