用OpenFlight API开发分形地形

分形是近年来流行的地形生成方法。用分形方法生成的地形具有速度快，逼真度高等特点。OpenFlight是视景仿真的标准三维模型格式，已经被大多数视景仿真软件接受。论述了如何用OpenFlight API和分形算法生成三维地形模型。     

T-2毒素对软骨细胞合成一氧化氮的影响

目的探讨T-2毒素对软骨细胞合成一氧化氮(NO)的影响。方法胎儿软骨细胞体外培养,用MTT法检测软骨细胞存活率;用Griess重氮化法测定软骨细胞培养上清液中NO含量;用Western blot检测软骨细胞一氧化氮合酶(iNOS)的表达。结果T-2毒素在质量浓度在1-2 000μg/L的范围内,软骨细胞存活率对其呈较典型的浓度依赖关系和时间依赖关系,而且随着作用时间的延长,浓度依赖关系更为明显;T-2毒素刺激软骨细胞分泌NO和表达iNOS蛋白。结论T-2毒素引起的软骨细胞损伤和生长抑制与T-2毒素刺激软骨细胞表达iNOS蛋白和分泌NO增多有关。     

丙型肝炎病毒非结构蛋白3全长基因的克隆和表达

目的克隆和表达丙型肝炎病毒(HCV)非结构基因NS3,为进一步研究其基因功能奠定基础。方法设计特异性引物,以HCV全长cDNA质粒为模板进行PCR扩增,获得全长NS3基因。将其插入克隆载体pMD18-Tvector中,经酶切、PCR及测序鉴定后,与表达载体pET32a重组,后转入BL21菌株并诱导表达。采用SDS-PAGE电泳及Western-blot检测NS3基因的蛋白表达水平。结果含有NS3的重组体构建成功并在大肠杆菌中表达。结论运用原核细胞基因工程技术成功构建和表达了HCV非结构基因NS3蛋白,为尽一步研究HCVNS3基因功能奠定了基础。     

丙型肝炎病毒C区截短型基因原核表达载体的构建及在大肠杆菌中的表达

目的建立稳定表达丙型肝炎病毒(HCV)核心蛋白的原核表达系统,获得高产量的纯化核心蛋白。方法应用多聚酶链反应(PCR),以HCV-H株全长cDNA序列为模板,扩增获得C区羧基端部分缺失的基因片段,克隆入原核表达载体pBVIL1,构建原核表达载体pBVIL1-Ct,转化HB101宿主菌,通过温度诱导表达截短型C蛋白。结果扩增得到目的基因长度为462bp,构建pBVIL1-Ct表达载体,在HB101宿主菌中通过温度诱导获得稳定表达,表达蛋白占菌体总蛋白含量的24%。Western-Blot及ELISA检测证实表达产物可与HCV患者阳性血清发生特异性结合反应。结论羧基端部分缺失的HCV C区基因片段可在大肠杆菌中稳定表达并具有良好的反应原性。     

高压(3～10KV)电机软起动模糊控制器

设计了一种新型的基于开关变压器的高压电动机软起动装置,对控制器的电路结构及软件进行了分析.异步电动机是一个非线性、强耦合的被控对象,运用常规的控制策略难以取得满意的控制效果,文中提出一种基于模糊控制规则可调整的高压异步电动机软起动器的设计方法,以确保异步电动机软起动具有最优化的动态和稳态性能.     

Operating strategy for gasoline/diesel dual-fuel premixed compression ignition in a light-duty diesel engine

Environmental problems have become a major issue for diesel engine development. Although emission aftertreatment systems such as DPFs (diesel particulate filters), LNTs (lean NOx traps) and SCR (selective catalytic reduction) have been used in diesel vehicles, the manufacturing cost increase caused by this equipment can be hard to be control. Thus, it is better for engine emissions to be reduced by improving the combustion system. A dual-fuel combustion concept is a recommended method to improve a combustion system and effectively reduce emissions. Low reactivity fuel including gasoline and natural gas, which was supplied to the intake port by the FPI (port fuel injector), improved the premixed air-fuel mixture conditions before ignition. Additionally, a small amount of high reactivity fuel, in this case diesel, was injected into the cylinder directly as an ignition source. This dual-fuel combustion promises lower levels of NOx (nitrogen oxide) and PM (particulate matter) emissions due to the elimination of local rich regions in the cylinder. However, it is challenging to control the dual-fuel combustion because the combustion stability and efficiency deteriorate due to the lack of ignition source and reactivity. Thus, it is important to establish an appropriate dual-fuel operating strategy to achieve stable, high efficiency and low emission operation. As a result of this research, a detailed operating method of dual-fuel PCI (premixed compression ignition) was introduced in detail at a low speed and low load condition by using a single cylinder diesel engine. Engine operating parameters including the gasoline ratio, a diesel injection strategy consisting of multiple injectors and timing, the EGR (exhaust gas recirculation) rate and the intake pressure were controlled to satisfy the low ISNOx (indicated specific NOx) and PM emissions levels (0.21 g/kWh and 0.1 FSN, 0.040 g/kWh, respectively) as per the EURO-6 regulation without any after-treatment systems. The results emphasized that a well-constructed dual-fuel PCI operating strategy showed low NOx and PM emissions and high GIE (gross indicated fuel conversion efficiency) with excellent combustion stability.     

The linear position tracking servo system using a linear voice-coil motor

More and more linear servo systems have been used in servo applications. Direct drive technology can greatly increase the bandwidth and the tracking accuracy. A position servo-system based on linear voice-coil motor was designed for one linear oscillation movement application. Besides the conventional position, speed and current control loops, the speed and acceleration feed-forward control of command position signal were also used. The experimental test proved the correctness of the design, and the system can track the given periodic sinnsoid position command signal of 15Hz with high accuracy. The linear voice-coil motor is very suitable for short stroke position tracking application with high dynamic response.     

浅埋大断面黄土隧道下穿既有铁路施工技术

在高桥隧道出口下穿南同蒲既有铁路施工中,根据黄土的特性对施工方案进行了优化,将设计的在总长100 m、φ159大管棚超前支护条件下采用双层支护形式的(外层为喷混凝土,内层为模筑混凝土)双侧壁导洞开挖法优化为双层支护形式的(两层均为喷混凝土)弧形导洞三台阶七步开挖法,并通过仰拱和二次衬砌紧跟的方法,开创性地解决了在大断面黄土隧道下穿构筑物施工中安全和进度的矛盾,有效地控制了洞内、外沉降变形,保证了下穿段隧道的安全快速施工和既有铁路的安全运营,并降低了工程造价,丰富了黄土隧道设计施工的理念。     

基于晶闸管串联的高压电机软起动

介绍了基于晶闸管串联的高压异步电动机软启动器,并推导出串联晶闸管的数学模型,应用该等效模型对高压电动机的软启动过程进行了仿真,为分析和设计基于晶闸管串联的高压异步电动机软启动器提供了一个有力的工具.     

一种新型DPF-SCR柴油机后处理技术

以柴油机尾气中NOx和碳烟颗粒(PM)两种主要污染物的后处理技术为重点研究对象,针对近年来正在应用及研究的柴油机的NOx和碳烟颗粒(PM)排放后处理控制技术,重点介绍了Nissan Diesel公司新近研制的一种新型的尿素选择性催化还原(SCR)柴油机微粒过滤系统的工作原理及特点,详细叙述了该系统通过改善催化剂材料、提...