丙型肝炎病毒核心蛋白基因的克隆及表达

目的在大肠杆菌中表达丙型肝炎病毒(HCV)核心蛋白基因片段。方法以HCV全长cDNA质粒为模板进行PCR扩增,获得366bp的核心蛋白基因片段。将其插入连接载体pMD18-T vector中,酶切后再与表达载体pBV220连接,构建重组表达载体pBV220/HCV-C。经鉴定后温度诱导表达,采用SDS-PAGE电泳及Western-blot检测核心蛋白基因的蛋白表达。结果经温度诱导后获得目的基因的非融合表达,SDS-PAGE电泳显示在14000 u处有一表达条带;Western-blot结果显示其具有HCV抗原特异性。结论丙型肝炎病毒核心蛋白基因成功表达,对临床诊断试剂和HCV基因疫苗的研制有一定的意义。     

T梁外观质量缺陷与防治

针对预制T梁外观质量缺陷,分析成因并提出修补方法和预防措施,从而使T梁的外观质量有很大提高。     

浅谈施工企业投标策略与技巧

报价是企业投标文件的重要组成部分之一,本文针对企业在投标过程中的投标策略与报价技巧做简单论述.     

Experimental investigation into low-velocity water entry of cylinder structure

The process of low-velocity water entry is utilized on a large scale for the military and engineering purposes. However, there are rarely systematic experimental investigations into the low-velocity water entry of cylinder structure for reference. In order to obtain typical phenomena and relevant laws, we design a set of experimental facilities with adjustable parameters and better repeatability to study this process with a high-speed photography system. The influences of cylinder radius, initial velocity and entry angle on the process of lowvelocity water entry are tested. Results show that six typical phases exist in this process: structure submersion,necking, cavity formation, cavity abscission, spray at the free surface and jet formation. Three factors mentioned above are key parameters and influence the process in different degrees, and some laws obtained in this paper have a reasonable agreement with the theoretical results. Our results provide references for the relevant numerical researches and engineering applications.     

Nine pathogenic fungi of waterhyacinth isolated in China

The native pathogens of waterhyacinth in China, were studied and compared on pathogenicity by Koch＇s postulate. Nine pathogenic fungi, YBH, YBB, YB, YYX, YY, YBA1, YBA2, YBA3 and YYB12, were isolated from diseased waterhyacinth plants, and collected from Zhejiang province and Shanghai. According to cultural characteristics, the nine isolates were preliminarily identified. Isolates YBH and YBB were Collectotrichum sp.; YB, YYX and YY were placed in fungi imperfecti; the isolates YBA1, YBA2, YBA3 and YYB12 were Alternaria sp. The isolate YBH was the highly virulent with a disease index （DI） of 65.28% after one month inoculation. The isolate YBA3 was equily virulent, with the disease index of 6770 after 7 day introduction. These two pathogens appear to have the potential as biocontrol agents and they deserve further study.     

船舶智能化研究现状与展望

智能化是船舶发展的重要方向。船舶智能化是在综合传感、通信、信息、计算机等多种先进技术的基础上,结合船舶具体应用环境,构建基于大数据、信息物理系统和物联网等特征的智能系统,使船舶航行、管理与服务更高效、更低秏、更安全和更环保。从大数据、信息物理系统、物联网三个方面介绍了船舶智能化的主要特征。从船舶智能航行和船舶智能管理与服务两个方面分析了船舶领域智能化现状和展望。船舶智能航行方面,探讨了综合船桥系统和无人驾驶的发展方向;船舶智能管理与服务方面,分析了水路ITS、交通流理论和数据分析方法的最新发展,并提出了基于可视分析解决船舶管理大数据处理的流程。     

数值分析在隧道支护中的应用

通过对某一隧道的施工形式及其所采用的支护方进行了数值分析,分析不同的支护参数对隧道周边应力和位移的影响,得出了有关塑性区分布与支护方式之间的关系,并对各类支护方式进行了比较与分析.     

基于神经网络的汽车结构件低载强化特性确定

提出了一种基于径向基函数神经网络的汽车结构件低载强化特性确定方法。通过足够样本的训练,构造出一个有效的神经网络模型。对于给定的强化载荷和强化次数,应用构造的神经网络模型,可以确定相应的低载强化效果。经过对某汽车前梁和传动系齿轮低载强化特性的试验验证,证实了该神经网络可以准确快捷地预测不同强化载荷和强化次数下结构的强化效果。简化了计算过程,提高了计算精度,为基于低载强化的汽车产品轻量化设计带来了方便。     

Survey of ship platoon cooperative control北大核心CSCD

船舶列队是未来水路运输的重要方式。分析船舶列队协同控制的特点和原理;从船−岸协同交互、列队控制模型、列队运动控制、列队典型应用等4个方面分别进行现状分析与梳理。总结当前船舶列队控制技术瓶颈,包括人机共驾控制、列队控制运动不确定性建模、列队协同控制一致性、通信受限下船舶列队鲁棒控制及一致性控制等。最后,指出未来的船舶列队协同控制发展,应重点解决基于数据驱动与机理融合的列队运动建模、基于生物群体机制的船舶列队控制、基于分层控制的船舶列队控制等关键问题。