经皮冠状动脉成形术对冠状动脉内皮功能的影响

目的　了解冠心病患者冠状动脉血管内皮的功能状态以及普伐他汀对经皮冠状动脉成形术 (PTCA)术后血管内皮功能的影响。方法　冠心病患者 30例均经冠状动脉造影证实冠状动脉有单支或二支以上≥ 70 %狭窄。随机分成普伐他汀组和非普伐他汀组 ,测定其PTCA术后即刻、5、10、15、2 0min冠状静脉窦血以及同时相外周血中内皮素 (ET)及一氧化氮 (NO)含量并进行比较。结果　PTCA术后即刻、5、10、15、2 0min冠状静脉窦血中ET含量较同时相外周血中高 (P <0 .0 1) ;NO含量较同时相外周血中低 (P <0 .0 1)。PTCA术后普伐他汀组冠状静脉窦血及外周血中ET含量较非普伐他汀组同时相低 (P <0 .0 1) ;NO含量较非普伐他汀组同时相高 (P <0 .0 1)。结论　PTCA术后应用普伐他汀可改善冠状动脉内皮功能。     

潜艇储备浮力论证研究方法分析

运用模糊综合评判方法对潜艇储备浮力的选取进行论证评估,讨论了在“小分舱大储备浮力”以及“大分舱小储备浮力”两种不同观点下潜艇储备浮力论证的评估模型.通过对航速、水面抗沉性、结构和系统3个不同因素对潜艇储备浮力影响的模糊评估分析,对各种储备浮力值的优劣进行评判,确定储备浮力的最佳值,为潜艇储备浮力设计的选优提供依据.     

公交线路客流OD矩阵推算方法研究

公交线路客流OD矩阵是公共交通管理和控制不可缺少的信息.参照机动车OD矩阵反推技术,对公交线路客流OD矩阵推算进行了专门研究.在公交线路客流OD调查数据的基础上,分析得到居民公交出行规律.首次建立了根据公交乘客上下车人数推算公交线路客流OD矩阵的极大熵模型,分别采用原始资料作为先验OD和重力模型形式的先验OD对模型进行求解,结果证明该模型精度较高.     

夯实水泥土桩复合地基技术

结合石家庄市制酒厂住宅楼工程复合地基施工,主要介绍夯实水泥土桩复合地基的设计原理和施工工艺,重点阐述通过静载荷试验确定复合地基的承载力标准值和变形模量,以验证复合地基设计的准确度。     

CLONING SEGMENT SPIKE PROTEIN GENE OF SARS-COV AND ITSEXPRESSION IN ESCHERICHIA COLI

Objective Expressing and purifying the seglnent of SARS-CoV spike protein in E. Coli Methods The target gene was obtained by RT-PCR. The PCR product was cloned into pEGM- T Easy Vector, sequencing and double restriction digestion (BamH Ⅰ , Pst Ⅰ) were performed. The target gene was subcloned into PQE30 expression vector. The gene was expressed in the E. coli strain M15 cells induced by IPTG. The protein was purified with a nickel HiTrap chelating metal affinity column. Results The recombinant expression plasmid was successfully constructed and the protein was well expressed in E. coli strain M15 cells. The ideal pure protein was obtained by purification. Western blotting analysis suggested the protein could act with the convalescent sera of lab confirmed SARS patients. Conclusion The segment of SARS-CoV spike protein was well expressed and purified, and can be applied in diagnosis and immunological research of SARS.     

Heuristics for the identical machine scheduling problem with preventive maintenances

In this paper, two mixed integer programming models integrating production scheduling and preventive maintenances are proposed to derive the optimal solutions for the identical machine scheduling problem with unavailability constraints. In the first model, the maintenance activities are performed periodically and the objective is to minimize the makespan. In the second model, the maintenance activities are flexible and the machines’ continuous working time cannot exceed a maximum allowed time T; the objective is to minimize the total completion time of jobs. For the first problem, we propose a heuristic longest batch time (LBT) and prove that the worst case error bound of LBT is 2. For the second problem, we develop a heuristic modified smallest processing time (MSPT) based on some properties of the optimal solutions. Computational experiments show that both of the heuristics are effective and efficient compared with the results obtained by CPLEX and the other algorithms.     

汽车维修市场特性分析及管理对策研究

汽车维修市场作为道路运输市场的重要组成部分，有着自身的特性，对此应认真加以研究，培养和健全汽车维修市场新体系，提高管理水平。     

大学生创造性思维培养初探

培养学生的创造性思维是目前我国教育界研究的热点问题。什么是创造性思维，创造性思维有什么特点，培养创造性思维的意义，如何培养学生的创造性思维，以及教学实践中有哪些可行性的操作方法。这些是本文所关注和要探讨的问题。     

β-分泌酶底物肽反转录病毒载体及其包装细胞株的构建

目的用pLXSN反转录病毒载体构建介导β-分泌酶底物肽(BACEsp)基因表达的pLXSN-BACEsp反转录病毒载体,并建立高效、稳定表达BACEsp的包装细胞株。方法根据BACEsp基因序列设计引物,PCR合成的BAC-Esp片段和pLXSN载体用EcoRⅠ、BamHⅠ双酶切后连接,构建成表达BACEsp的pLXSN-BACEsp反转录病毒载体。用脂质体介导转染PT67包装细胞,经抗性筛选后,挑单克隆扩大培养,用NIH3T3细胞测定病毒滴度,筛选出高效产毒细胞株。结果经酶切、连接后构建成的质粒称为pLXSN-BACEsp,经测序证实构建成功,无任何碱基突变。经脂质体介导转染包装细胞、抗性筛选和测定病毒滴度,筛选出具有高病毒滴度的细胞集落,扩大培养后建立BAC-Esp高效表达的包装细胞株。结论成功构建了能表达BACEsp的pLXSN-BACEsp反转录病毒载体,并建立了稳定、高效地产生有活性的BACEsp产毒包装细胞株。     

气垫调压室气室常数敏感性分析

气垫调压室“等POV0值”的控制方法综合考虑了水位、气压的调节变化范围,给实际运行带来了许多便利,对于气垫调压室设计来说,选择—个合适的气室常数对简化运行管理,保证工程安全,具有十分重要的意义。本文就某一气垫调压室水电站的气室常数的敏感性进行分析计算,最后得到满足电站稳定运行的气室常数。