牙龈卟啉菌蛋白酶rgp Acd基因表达载体的构建

目的构建牙龈卟啉菌蛋白酶rgpA催化结构域(rgpAcd)基因的表达载体。方法利用PCR技术和基因重组技术,克隆牙龈卟啉菌蛋白酶rgpAcd,插入中介载体pMD18-T中并测序鉴定。将目的基因片断插入原核表达载体pET-15b,构建表达质粒pET-15b/rgpAcd,通过限制性酶切鉴定。结果PCR产物电泳结果显示,在大约1.5 kb处有一特异的条带,与预期的大小一致,核酸序列测定与分析的结果表明,克隆的1 476 bp基因序列与GenBank数据库中的序列呈现100%同源性,未发生任何突变;构建的表达质粒经酶切后所得片断与预期大小一致,表明牙龈卟啉菌蛋白酶rgpAcd基因的表达载体构建成功。结论成功克隆了牙龈卟啉菌rgpAcd基因并构建了表达载体,为进一步研制重组活载体疫苗奠定了基础。     

两自由度不同截面形式柱体涡激振动的CFD数值模拟

文章采用计算流体力学（CFD）方法,结合SST k-棕湍流模型,对低质量比柱体进行两自由度涡激振动数值模拟,得到了柱体升力、曳力系数的时程曲线,并观察了柱体进入锁振状态的幅值变化,研究了不同截面形式柱体在外流速处于0.1-1.0 m/s范围内的振动响应。将圆柱体在不同流速下两向振动的CFD数值模拟与实验数据进行比较,得到了较为满意的结果。通过分析不同截面柱体在不同外流速下的振动幅值发现,带有抑振装置的柱体截面形式能够有效地减小涡激振动,其中,板状截面柱体抑振效果较好。     

Simulation of hydrodynamic performance of drag and double reverse propeller podded propulsors

The unsteady performance of drag and double reverse propeller podded propulsors in open water was numerically simulated using a computational fluid dynamics (CFD) method. A moving mesh method was used to more realistically simulate propulsor working conditions, and the thrust, torque, and lateral force coefficients of both propulsors were compared and analyzed. Forces acting on different parts of the propulsors along with the flow field distribution of steady and unsteady results at different advance coefficients were compared. Moreover, the change of the lateral force and the difference between the abovementioned two methods were mainly analyzed. It was shown that the thrust and torque results of both methods were similar, with the lateral force results having the highest deviation     

Comparing prediction methods for maritime accidents

ABSTRACT

The purpose of maritime accident prediction is to reasonably forecast an accident occurring in the future. In determining the level of maritime traffic management safety, it is important to analyze development trends of existing traffic conditions. Common prediction methods for maritime accidents include regression analysis, grey system models (GM) and exponential smoothing. In this study, a brief introduction is provided that discusses the aforementioned prediction models, including the associated methods and characteristics of each analysis, which form the basis for an attempt to apply a residual error correction model designed to optimize the grey system model. Based on the results, in which the model is verified using two different types of maritime accident data (linear smooth type and random-fluctuation type, respectively), the prediction accuracy and the applicability were validated. A discussion is then presented on how to apply the Markov model as a way to optimize the grey system model. This method, which proved to be correct in terms of prediction accuracy and applicability, is explored through empirical analysis. Although the accuracy of the residual error correction model is usually higher than the accuracy of the original GM (1,1), the effect of the Markov correction model is not always superior to the original GM (1,1). In addition, the accuracy of the former model depends on the characteristics of the original data, the status partition and the determination method for the status transition matrix.     

绿色交通节能效益量化的问题与对策

研究了绿色交通节能效益量化的问题及其成因,提出了对策建议。     

考虑非稳态传热的高速汽油机主轴承润滑分析

针对一款高速汽油机主轴承内部润滑与摩擦磨损问题,考虑到轴承承载不均导致的轴瓦与润滑油非稳态传热,基于弹性流体动力润滑(EHD)和轴承动力学理论方法,通过迭代计算,得出该高速汽油机具有代表性的第三主轴承在最大转速(9500 r/min)时轴承内部精确的温度场与热变形,并以此为轴承新的几何轮廓边界条件分析轴承的实际润滑情况.结果表明,与未考虑轴瓦温度场及热变形相比,轴承润滑状态明显恶化,具体表现为轴承最小油膜厚度减小、最大油膜压力增大,且出现较严重的磨损.最后通过发动机台架试验测得轴承的实际工作情况,并与计算结果进行对比,计算结果与实际摩擦磨损情况吻合,验证了所用方法和所得研究结论的正确性.     

使用UG NX4.0三维软件设计客车车架

客车车架是客车底盘的重要组成部分,通常是用二维软件设计,有一定的局限性。本文介绍使用UG NX4.0三维软件对车架进行设计。     

路基回弹模量对路面结构层受力的影响分析

通过计算不同路基回弹模量下的弯沉系数、上层底面弯拉应力系数、中层底面弯拉应力系数以及三层体系表面最大剪应力系数,得出了这些系数和路基回弹模量的关系.     

套箍法加固石拱桥主拱圈的正截面承载力的理论分析

针对石拱桥拱圈加固后的正截面承载力计算问题,提出了考虑加固前截面受力状况及加固材料不同力学性能的计算方法。对加固后的拱圈进行应力应变分析,得出界限破坏的3种形式,对每种界限破坏形式利用截面的协调变形和截面材料的应力应变关系,分析了其极限状态时组合截面的相对界限受压区高度,得出加固后石拱圈最后的破坏模式主要与加固前的初应变及加固层的厚度有关。对应于3种破坏形态从理论上推导出套箍法加固石拱圈在二次受力时的正截面承载力计算公式。计算公式充分考虑了加固前的构件应力水平、加固后截面不同材料组成及构件破坏形态的影响,比现有加固规范的计算取值更趋于合理,为同类加固结构的设计和计算提供了依据。     

基于Wilson熵分布假设的交通公平性量化评价模型

在对交通公平性的量化评价方法进行考察的基础上,从效益分配符合"W ilson熵分布"的假设出发,尝试建立了群体决策型的公平性量化评价模型,该模型综合考虑了区域公平性和不同群体效益归属公平性.对于模型的分析表明交通设施的群体决策型公平性量化评价模型具有最佳解.同时通过数学方法可以证明建立的基于"W ilson熵分布"假设的交通公平性量化评价模型能很好地体现对于弱势群体和不发达区域的效益"补偿"作用.分析结果表明该模型用于交通公平性评价是合理、可行的.