葎草花粉cDNA表达文库的构建和初步鉴定

目的构建葎草花粉cDNA表达文库,为进一步筛选葎草花粉的主要致敏蛋白组分、制备重组变应原奠定基础。方法采集新鲜葎草花粉,过筛后液氮保存,利用Trizol法抽提葎草花粉总RNA,纯化后PCR法反转录合成cDNA。经SfiⅠ酶切,过凝胶柱层析,去除小于400 bp的片断,收集大于400 bp的cDNA片段,加工后与λTripIEx2连接,文库体外包装,取出一小部分感染宿主菌XL1-Blue MRF,检测文库容量;PCR分析插入的cDNA片段的大小及多样性。结果成功构建一个含有5×105重组子的葎草花粉表达文库,重组率为90%,平均插入片段长度约为1.02 kb。结论所建文库容量及插入片段大小合适,适用于筛选克隆的目的cDNA。     

55岁以上自然人群中帕金森病患者认知功能障碍的临床研究

目的　了解 55岁以上自然人群中帕金森病 (Parkinson sdisease ,PD)患者认知功能障碍的发生情况。方法　以流行病学调查资料为基础 ,采用非配对的病例对照研究方法 ,分析 55岁以上自然人群中PD患者认知功能障碍的发生率 ,评估认知功能障碍发生的相对危险性。结果　 55岁以上自然人群中 ,PD组和非帕金森病 (noParkinson sdis ease,NOPD)组认知功能障碍的发生率分别为 2 7.78%、5.55% ;年龄调整的发生率分别为 16.2 0 %、9.87%。与同一自然人群中NOPD组比较 ,PD组发生认知功能障碍的危险性明显增高 ,但主要增加低年龄组 (55～ 74岁 )中老年人发生认知功能障碍的危险性 (OR =8.59,95%CI :3 .13～ 2 3 .80 ) ;而且PD是低年龄中老年人发生认知功能障碍和痴呆的独立危险因素 (OR =6.2 7,95%CI :2 .3 5～ 19.2 7;OR =5.50 ,95%CI :1.52～ 19.94)。年龄调整的MMSE分值的均值PD组明显低于NOPD组 (P <0 .0 0 1)。结论　PD主要增加低年龄 (55～ 74岁 )中老年人认知功能障碍发生的危险性 ,而且PD认知功能障碍是疾病本身所致     

人脾脏巨噬细胞的分离与纯化

目的　探索分离纯化大量人脾脏巨噬细胞 (MΦ)的方法。方法　选取 10例手术切除的脾脏 ,机械研磨法获得脾脏组织细胞混悬液。采用贴壁培养的方法进行MΦ的分离与纯化 ,相差显微镜下动态观察 ,明确贴壁培养的合适时间 ,详细记录MΦ分离纯化过程各步骤所需时间。收获的MΦ ,台盼蓝染色判定活力并计数 ,统计出平均每克脾脏组织收获的MΦ数及其纯度。相差显微镜、透射电镜观察分离纯化MΦ的形态特征。结果　分析贴壁培养各时间段的细胞纯度及活力 ,确定MΦ培养的合适时间为 2～ 3h。贴壁培养纯化后 ,平均从每克脾脏组织可收获 (0 .2 5± 0 .0 3)× 10 8个MΦ ,纯度为 (90± 5 ) % ,细胞活力≥ 99% ,细胞结构及各种细胞器结构均完整。结论　贴壁培养方法分离纯化人脾脏MΦ是成功可行的 ,收获的MΦ数量大、纯度高、活力好 ,为脾脏MΦ功能的深入研究奠定了基础     

宁波地区土层电阻率特性及主要参数相关性研究

结合宁波轨道交通1、2、3号线岩土工程勘察,利用Wenner等距四极法测定土体电阻率,研究了含水率、孔隙比、压缩模量对各土层电阻率影响及相关性,结果表明:宁波地区软黏土电阻率与物理力学指标含水率、孔隙比、压缩模量之间的相关性较好,土的电阻率随含水率、孔隙比的增大而减小,随压缩模量的增大而增大;不同土层的电阻率不同,黏土及粉质黏土的导电性优于砂土,为轨道交通供电、变配电、接触网及弱电设计提供依据。     

Comparison of advanced imputation algorithms for detection of transportation mode and activity episode using GPS data

Global Positioning System (GPS) technologies have been increasingly considered as an alternative to traditional travel survey methods to collect activity-travel data. Algorithms applied to extract activity-travel patterns vary from informal ad-hoc decision rules to advanced machine learning methods and have different accuracy. This paper systematically compares the relative performance of different algorithms for the detection of transportation modes and activity episodes. In particular, naive Bayesian, Bayesian network, logistic regression, multilayer perceptron, support vector machine, decision table, and C4.5 algorithms are selected and compared for the same data according to their overall error rates and hit ratios. Results show that the Bayesian network has a better performance than the other algorithms in terms of the percentage correctly identified instances and Kappa values for both the training data and test data, in the sense that the Bayesian network is relatively efficient and generalizable in the context of GPS data imputation.     

Design and power flow analysis for multi-speed automatic transmission with hybrid gear trains

A design approach for multi-speed automatic transmission with hybrid gear trains is proposed. The approach can be used to design automatic transmissions composed of planetary gear trains, fixed-axis gear trains or hybrid gear trains and the shifts follow the “one controlled part changing rule”. This approach consists of four parts: determining graphical configuration of the system using hypergraphs, analyzing power flow formulation, obtaining shifts configurations by shift searching algorithm, and determining physical configuration. A nine forward speed automatic transmission is analyzed to highlight the capacities of the proposed approach. The results have shown that the suggested approach is much clearer and less time consuming than the methods currently used.     

考虑温度效应的磁流变阻尼器的Bouc-Wen模型

由于能量耗散生热、环境温度以及通电线圈产生的热能会使磁流变阻尼器在很大的温度范围内工作,温度对磁流变阻尼器输出阻尼力有很大影响,因此有必要实验研究温度对磁流变阻尼器输出特性的影响和建立能在较大温度范围预测磁流变阻尼器阻尼力的数学模型。本文从Bouc-Wen模型出发,通过温度实验数据,建立了能在较大电流范围和温度范围内使用的数学模型,并用1stOpt软件的通用全局优化算法识别模型参数,验证表明该模型能很好地体现磁流变阻尼器不同温度时的阻尼特性。     

橡胶沥青开级配排水沥青混凝土（RA-OGFC）的试验研究

采用橡胶沥青（RA）作为胶结料,进行开级配排水沥青混凝土（OGFC）配合比设计;对橡胶沥青及RA-OGFC的性能进行评价,并考察抗车辙剂和纤维对RA-OGFC高低温性能的影响。结果表明,添加抗车辙剂和纤维可显著改善混合料的高温性能,弥补RA-OGFC混合料高温稳定性的不足。     

透析电起动机工作特性探讨使用因素对其启动功率的影响

简要分析了串激式直流电动机的工作特性。指出忽视使用因素对起动机工作特性,特别是对电起动功率的影响,是造成内燃机电起动系统起动功率不足、起动困难、起动机发热等故障的主要因素之一。     

In-plane Auto-Parametric Vibration of Inclined Cables under Random Transverse Excitation

In-plane auto-parametric stochastic vibration of inclined cables subjected to Gaussian white noise in transverse bridge orientation is investigated. Based on Newton＇s laws of motion and Galerkin＇s modal truncation principle, the influences of geometry nonlinearity induced by sag and large displacement of cables and the initial equilibrium state are taken into account. Meanwhile, the three-dimensional non-linear differential equations of inclined cables for coupling vibration are deduced, equivalent stochastic linearization method is applied to derive the 14-dimensional first-order nonlinear differential equations of state vectors, and the Runge-Kutta integration method is utilized to obtain the root mean square （RMS） response. Results show that when the transverse random excitation imposed on the stayed cable exceeds a critical value, the in-plane transverse vibration of the cable are excited due to tim auto-parametric nonlinear coupling, and the critical value of random excitation increases with the damping ratio. In this motion, the cable response possesses non-stationary characteristics, even though the loading keeps stationary.