Asymmetric PCR method in generation of HBV ssDNA for pyrosequencing

Objective To explore the optimal primer ratio and concentration of asymmetric polymerase chain reaction (A-PCR) in producing hepatitis B virus (HBV) single-stranded DNA (ssDNA) for pyrosequencing. Methods A-PCR was carried out to generate HBV ssDNA with forward to reverse primers of different ratios (50 : 1, 100 : 1) and concentrations (13. 0 pmol/25μL and 0.14 pmol/25μL, 19. 5 pmol/25μL and 0. 21 pmol/25μL), and the product yield and quality were compared respectively. Results The forward to reverse primer ratio of 50 : 1 provided better yield and concentration of 19. 5 pmol/25μL and 0. 21 pmol//25μL generated a clearer band. Conclusion A simple and feasible method to produce HBV ssDNA for pyrosequencing in batch is established.     

Union examination of AFP, AFU, AFPL3 and γ-GT in early diagnosis of primary liver cancer

Objective To explore the significance of union examination of blood serum liver cancer tracers in the early diagnosis of liver cancer. Methods We observed and compared the level of blood serum liver cancer tracers armor embryo protein (AFP), crag algae glycosidase (AFU), armor embryo protein heteroplasmon (AFPL3) andγ-Gu Anxian transferase (γ-GT) in early time for primary liver cancer patients and hepatitis liver cirrhosis patients and those chronic hepatitis B patients who had liver cancer family history. Results Finally among the 30 patients in the early liver cancer group, 23 were positive with AFP, 20 with AFU, 15 with AFPL3 and 21 with γ-GT. Five were found positive with blood serum AFP, AFPL3, AFU and γ-GT at the same time; 5 with AFP, AFPL3 and γ-GT; 5 with AFP, AFU and AFPL3; 7 with AFP, AFU andγ-GT. By contrast, in the control group, among the 30 hepatitis liver cirrhosis patients and those chronic hepatitis B patients with liver cancer family history, 11 were found positive with AFP, 3 with AFPL3, 12 with AFU and 14 with γ-GT. None of the patients were found positive with union examination of AFP, AFPL3, AFU and γ-GT in the blood serum at the same time. Conclusion The union examination of AFP, AFU, AFPL3 and γ-GT is significant to the early diagnosis of primary liver cancer.     

国际化视野下航海类高职院校的思想政治教育改革

文章基于航海类高职院校思想政治教育的特点,就全球化背景下如何充分发挥思想政治教育的德育功能,更好地服务于高职人才培养工作,从思想政治教育的内容、方法和价值导向等方面进行积极探索,指出航海类高职院校思想政治教育必须适应全球化的形势,侧重于全球意识、爱国意识、规则（法制）意识、职业素质和创新意识教育,并在培养目标和教育方法等方面有所调整,以增强思想政治教育的针对性和有效性。     

混凝土徐变模型在桥梁预拱度设置中的分析比较

通过MIDAS有限元软件建立了悬臂法施工连续刚构桥梁计算模型,并考虑不同的混凝土徐变模型计算了桥梁的预拱度值,并与实际工程做对比,得出选择徐变模型的一些原则.     

上海长江大桥主航道斜拉桥分离式钢箱梁设计

上海长江大桥主航道桥采用主跨730 m的双塔双索面斜拉桥,人字形塔,分离式钢箱梁.介绍分离式钢箱梁设计中纵横梁的连接及轨道交通的特殊构造两大特点、主要节点构造及主要计算内容.     

黑龙江省农村客运市场存在的问题及对策分析

随着＂村村通＂工程的不断推进,黑龙江省农村客运市场得到了迅猛发展。但是,城乡一体化进程的加快和农民生活水平的不断提高,对农村客运也提出了更高的要求,其发展过程中存在的问题逐步显现出来。基于黑龙江省农村客运的现状,就黑龙江省农村客运发展中存在的问题提出相应的解决办法,对推进黑龙江省农村客运的发展具有一定的指导意义。     

基于解析结构模型的公交运营质量分析

将影响城市公共交通运营质量的错综复杂的因素看作一个大系统,利用系统工程中的解析结构模型,分析系统中各要素的直接、间接关系,画出多级递阶结构,并描述出各要素之间的相互关系,找出影响城市公共交通运营质量的根本影响因素,从而可以分析出导致公共交通出行分担率较低的主要原因.并提出解决的对策和具体措施.     

基于线框模型的白车身刚度定性仿真

提出一种参数化白车身线框几何模型的构建方法,基于此线框模型快速建立白车身刚度计算有限元模型,实现了概念设计阶段白车身刚度的定性评价.以某白车身为例,计算了概念设计阶段定性仿真模型与详细设计阶段定量仿真模犁的弯曲刚度和扭转刚度.计算结果表明,概念设计阶段和详细设计阶段的弯曲刚度与扭转刚度值均在相差10%左右.     

生物柴油的喷油正时调整特性

在一台四缸涡轮增压柴油机上进行了不同喷油提前角时燃用生物柴油、生物柴油与柴油混合燃料和柴油的动力性、燃料经济性和排放特性对比试验.结果表明,对于BDO和BDG25混合燃料,喷油提前角提前2°时动力性最好;对于BD100燃料,原机的喷油正时动力性最好;随喷油正时提前,3种燃料比能耗减小、碳烟排放减少、NOx排放增大;在高转速时HC和CO排放基本不变.     

汽车发动机舱散热的数值仿真分析

考虑了散热器和高温元件的散热量以及空气的对流传热和辐射等因素,建立了发动机舱模型.利用CFD软件对汽车发动机舱流场进行了数值仿真,得到了发动机舱内部气流的速度与温度分布和重要元件的表面温度等参数,最后对存在的问题提出改进方案.