Objective To prepare polyclonal antibody of transactivated protein 5 of hepatitis C virus nonstructural 5A (NA5ATP5) and to explore its expression in the liver tissues. Methods In Escherichia coil BL21, the prokaryotic expression vector pET32a(+)-NS5ATP5 was induced by isopropyl-β-D-thiogalactoside (IPTG), and it was analyzed with sodium dodecyl sulfate-polyaerylamide gel electrophoresis (SDS-PAGE) and Western blotting. And the purified protein was used to immunize the rabbit to prepare polyelonai antibody, with which we studied the function of NSSATP5 by determining the different liver tissues with the streptavidin-perosidase (SP) immunohistochemistry method. Results Recombinant NS5ATP5 (molecular weight: 65 kD) and polyclonal antibody were successfully prepared. NS5ATP5 expression in the liver of patients with chronic HCV infection was much higher than that of a normal person, and it was detected mainly in the cytoplasm. Conclusion The findings of the expression difference between HCV patients and normal people led to a novel diagnostic marker to detect HCV infection. 相似文献
The paper takes station-based bikesharing system (SBS) with docks and dockless free-floating bikesharing system (FBS) as two targets to dig out the relationship between users and use frequency of the services for each scheme, and how the relationship varies from scheme to scheme. To achieve this, studies are carried out focusing on three questions: “who are using these two bicycle services?”; “what are the factors influencing the use frequency of both bicycle systems?”; and “which specific level of the factors influencing the use frequency of both bicycle schemes?” To collect data from users, a survey was designed containing questions for user attributes and service experience and conducted jointly on-line and on-site at four locations with mixed land use in Hangzhou, China. Analysis results show that SBS and FBS have similar user structure but different factors influence use frequency. Based on analysis results, from the user perspective, SBS’s strength is to have good quality with low cost while FBS is more flexible and free to use. Finally, recommendations for SBS are to involve more technology to expand its range to aided bikes for senior citizens and open the access for a mobile renting system, whereas for FBS, it is critical to get government cooperation and for operators to add parking area restrictions into the cellphone application, and create an on-line platform where users can find all the free-floating bike information.
