EXPRESSION OF NESTIN AND GLIAL FIBRILLARY ACIDIC PROTEIN IN DIFFERENT PERIOD AFTER SPINAL INJURY IN ADULT RATS

Objective To study the expression of Nestin and glial fibrillary acidic protein (GFAP) in different period after spinal injury in adult rats. Methods Animal moels were created by artery clamp. Expression of Nestin and GFAP in different period ( 1,3,5days; 1-8 weeks) and different area( injury locus and its surrounding tissue ) after spinal injury were observed pathologicaly using immunofluorescence and LeicaQ5001W imaging processing system.Results There was expression of Nestin and GFAP in injured area 1 day after injury. The expression increased not only in in injured area but its sourrounding 3--7 days later and gradually got to peak value. As the time went on, expression of Nestin and GFAP dereased. Conclusion Spinal injury can induce the expression of Nestin. Nerve stem cell has response to spinal injury. There is positive correlation between expression of Nestin and hyperplasia of reactivity astrocyte. Nerve stem cell maybe invovled in the repair of central nervous system (CNS).     

城市公交线网优化研究

本文探讨了城市公交线网优化的重要指标,建立了公交线网优化的数学模型,并采用遗传算法对该模型进行求解,取得了良好的效果,可为中小城市公交线网规划提供参考.     

平面绕型对RFIC螺旋电感电性能的影响

基于RF集成电路硅衬底螺旋电感（SIOS）的典型物理模型,用HFSS（High Frequency Structure Simulator）对中空平面四边形、八边形和圆形螺旋电感的电性能进行了模拟计算,结果与理论分析吻合.在内半径相同的情况下,圆形螺旋电感最大品质因数Q分别比四边形和八边形增加约0.90和0.80倍;在最大品质因数频率点,圆形螺旋电感端口反射系数分别比四边形和八边形减小约32.5%和26.6%,金属材料用量分别比四边形和八边形减少约30%和20%;当Q〉5时,圆形螺旋电感的工作带宽分别比四边形和八边形增大约60%和30%.     

THE EXPRESSION OF BCL-2, BAX AND CASPASE-3 IN NEURON OF THE SPINAL CORD ANTERIOR HORN AFTER CAUDA EQUINA ACUTE COMPRESSION

Objective To explore the influence of the acute cauda equina compression on the iumbosacral spinal cord; To clarify the pathologic change of the motor neuron after acute cauda equina compression. Methods 27 canis familiaris were randomly divided into 9 groups （3 in each） ： one for normal group, one for control group, and seven for compression groups. The control group and compressed groups was given operation and the sac made of silica gel was placed under the lamina of L5-6. Water was injected into the sac until their posterior legs paralysis in compressed groups, the animals had been compressed for 4, 8, 12, 24, 48, 72, 168 hours. The control group were not injected water. Cells apoptosis was investigated with the technology of TdT-mediated biotin dUTP nick end-labeling （TUNEL） staining. The Bcl-2 Bax and Caspase-3 protein was investigated by immunohistochemical method. Results TUNEL staining cells in anterior horn presented after compressed 8-12 hours, and at 72 hours the number of positive cells got to maximum, it decreased subsequently after 168 hours. The protein of Bax, Bcl-2 expressed a little in normal motor neuron. The caspase-3 protein didn＇t express in normal ceil. They all reached the peak at 72 hours after compression. Conclusion The apoptosis of motor neuron occurred earlier after eauda equina acute compression. Bax protein restrained Bcl-2 protein then active caspase-3 and conduced apoptosis of motor neuron.     

脾虚证大鼠侧脑室注射孤啡肽对细胞免疫功能的影响

目的脾虚证大鼠侧脑室注射孤啡肽,研究其对细胞免疫功能的影响,从神经内分泌的角度探索脾虚的细胞免疫学变化本质。方法用100%大黄煎剂灌胃复制脾虚证大鼠模型,四君子汤作为反证药物,侧脑室埋管,注射孤啡肽,MTT法检测脾T淋巴细胞增殖能力、足跖增厚法检测迟发型变态反应程度,判定细胞免疫功能改变情况。结果灌服大黄煎剂后,大鼠逐渐出现腹泻,或伴有脱肛,食量减少,精神不振,懒动,被毛失去光泽,形体消瘦等脾虚症状,而空白对照组(BC组)没有上述症状;T淋巴细胞增殖能力、迟发型变态反应程度与BC组比较明显减弱(P<0.01)。四君子汤治疗组(DFMD组)和孤啡肽组(OFQ组)大鼠上述脾虚症状及检测指标明显改善(P<0.01)。结论脾虚证大鼠侧脑室注射孤啡肽能提高细胞免疫功能。     

我国大城市人口控制与教育资源配置的协同研究

几十年来，我国人口控制取得了举世瞩目的成就，部分大城市开始出现人口自然负增长，但是人口年龄结构却发生了峰谷交替的剧烈波动，从而对教育资源的合理配置产生了严重的冲击。本从人口结构角度出发，对我国37个大城市的人口与教育资源配置进行了协同研究，并根据上海市的超前性与典型性，提出了若干对策建议。     

BPON系统的ONT研究和嵌入式系统设计

宽带无源光网络（BPON）系统是一种优良的高速、宽带接入解决方案,是实现光纤到户（FTTP）的重要解决方案之一.在介绍BPON系统的网络分层结构基础上,分析了光网络终端（ONT）设备实现的主要功能,讨论了实现方案.利用嵌入式思想开发ONT系统,并研究了软件设计的主要方法.将软件划分为5个模块,设计出每一模块的流程,并讨论了其中的消息处理核心功能的实现过程.     

Improved Genetic Algorithm for Job-Shop Scheduling

Introduction Job shopschedulingproblem(JSP)isoneofthe well knownhardestcombinatorialoptimizationprob lems,andmanysolutionsbasedongeneticalgorithms(GAs)havebeenproposedbymanyresearchersso far[13].SincethedemonstrationofapplicationofGA toschedulingproblemsb…     

Catalytic Combustion of Lean Methane Assisted by Electric Field over Pd/Co<Subscript>3</Subscript>O<Subscript>4</Subscript> Catalysts at Low Temperature

A series of Pd/Co₃O₄ catalysts were prepared by Self-Propagating High-Temperature Synthesis (SHS) method in this study, and electric field was applied for catalytic combustion of lean methane over Pd/Co₃O₄ catalysts at low temperature. When electric field was applied, the catalytic combustion performance of Pd/Co₃O₄ catalysts was greatly improved, and the application of electric field could reduce the load of active element Pd to some extent while maintaining the same efficiency. Based on experimental tests and the analysis results of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), H₂-temperature-programmed reduction (H₂-TPR) and in-situ diffuse reflectance infrared Fourier transform spectroscopy (in-situ DRIFTS), the mechanism of catalytic oxidation of CH₄ over Pd/Co₃O₄ catalysts in electric field was proposed. The catalytic combustion of CH₄ occurs only when the temperature is higher than 250 °C normally, but when electric field was applied, the whole process of CH₄ oxidation was promoted significantly and the reaction temperature was reduced. Electric field could promote the reduction of the support Co₃O₄ to release the lattice oxygen, resulting in the increase of PdO_x and the surface chemisorbed oxygen, which could provide more active sites for the low-temperature oxidation of CH₄. Furthermore, electric field could accelerate the dehydroxylation of CoOOH to further enhance the activity of the catalysts.     

四台子大桥40m预应力混凝土工型梁安装施工

本文介绍四台子大桥40m跨径预应力混凝土工型梁安装方案的选定、施工方法及有关注意事项.