PROLIFERATION AND MIGRATION OF EPENDYMAL CELLS IN THE BRAIN OF ADULT RATS AFTER PERMANENT FOCAL CEREBRAL ISCHEMIA

Objective Ependymal cells are thought to be the primary source of neural stem cells in the adult central nervous system. The purpose of this study is to examine spatial and temporal profiles of ependymal cell proliferation and migration after focal cerebral ischemia. Methods Eighty male Sprague Dawley rats underwent permanent middle cerebral artery occlusion after injection of 10/μL of 0.2% Dil into the lateral ventricle. Rats were sacrificed and brain sections were acquired for pathological evaluation and laser confocal imaging at day 1,3,7,11,14,21 and 28 after ischemia. Results The density of Dil-labeled cells in the ischemic ipsilateral subventricular zone was significantly higher than that in the control group and these labeled cells dispersed in the ischemic ipsilateral subventricular zone and/or were located in ependyma from day 1 to 11. In the ischemic ipsilateral cortex, some Diilabeled cells occurred in peri-infarction and infarction of parietal region at day14 and peaked at day 21 when some Dil-labeled cell nodules were found in this region. During postischemic day 14--28, a significant decrease in labeled celldensity in the ischemic ipsilateral subventricular zone was coincident with a significant increase in labeled cells density in the cortex (peri-infarction and infarction). Conclusion The results indicate that ependymal cells proliferate and migrate after focal cerebral ischemia in the adult rat brain.     

A New Proposal of Inverse-Time Characteristics for Overcurrent Relay Based on Microcomputer

The conventional time function of electromechanical relays is hard to coordinate with other relays．In order to promote the application of inverse-time overcurrent relays，a new time function for microprocessor-type relay is proposed．The setting of the trip time for this relay is performed by determining the shortest trip time and the longest trip time，respectively．The results of analysis show that with the new time function，the inverse-time overcurrent relay is easy to coordinate with other relays and has a comparatively shorter trip time，and that the fault happens in the protective zone．     

论完善民事诉讼简易程序

程序繁简分立是从总体上提高诉讼效率的有效途径。为提高诉讼效率，必须改革和完善民事诉讼的简易程序，解决“简易程序不简易”、“普通程序不规范”的问题。     

电磁波在大气非平衡等离子体中的吸收

采用平板模型对入射电磁波在大气非平衡等离子体中的吸收特性进行了数值模拟,着重研究了大气中的负离子的存在、外加磁场以及碰撞频率等因素对电磁波吸收的影响,并给出了相关参数的数值模拟结果.     

Efficient iris recognition via ICA feature and SVM classifier

With the increasing demands of security in ournet worked society,the technology for personalidentification works as the main solution to safe-guard people s properties.Biometrics is an alterna-tive to solve the problemand has the advantage thatthey cannot be stolen or forgotten like pass words.Because personal identification numbers or identifi-cation tokens(such as s mart cards)cannot provide ahighlevel of security which can be copied,inspec-ted and/or stolen.They only showthe knowledgeof som…     

民间资本介入地方公共产品供给的理论思考

随着改革深化，地方公共产品的供给主体发生了很大的变化，民间资本也开始渗透到原本属于财政投资的领域，介入到地方公共产品供给。从理论上到实践中，民间资本介入地方公共产品的供给都有其存在的依据，这是由地方公共产品的性质决定了的，也是在经济发展演进中政府投资范围的不断调整所必然要求的，有其存在的可能性和必要性。民间资本介入地方公共产品供给，大大改变了地方公共产品供给的模式和效率，同时也面临着诸多障碍，但民间资本介入地方公共产品的供给将是不可改变的趋势。民间资本介入地方公共产品的模式、范围、规模仍将会有较大的发展。     

梁式转换层异型柱框架的地震作用效应

采用底部2层为大空间商场、上部为住宅的高层建筑作为建模分析对象，模拟实际工程情况，按国家有关设计规范要求，针对抗震设防烈度7度和8度、场地土Ⅰ～Ⅳ类等不同组合，采用振型分解反应谱法和弹性时程分析法分析计算了72例钢筋混凝土梁式转换层异型柱框架高层建筑，对受小震作用的钢筋混凝土梁式转换层异型柱框架体系的层变形、层剪力及基底倾覆力矩变化规律进行了分析，得出若干可供设计参考的结果。     

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国内外综合交通信息平台的发展可以大致归纳为三类模式：政府行为型、政府建设-企业运营型、政府引导-企业运作型。本文以北京市交通综合信息平台及相关信息服务系统为实例，在分析其发展情况的基础上，深入研究了综合交通信息平台政府行为型发展模式的建设机制、运营机制、服务机制、可持续发展机制以及优缺点、适用性。     

PROPERTIES OF PROLIFERATION AND DIFFERENTIATION OF NEONATAL RAT RETINAL PROGENITOR CELLS IN VITRO

Objective To investigate the properties of proliferation and differentiation of neonatal rat retinal progenitor cells （RPCs） in vitro. Methods RPCs were isolated from neonatal SD rats neural retina and cultured in DMEM/F12 ＋ N2 with EGF and bFGF （suspension medium ） or 10 % FBS without EGF and bFGF （ differentiation medium）. The cells grew as suspended spheres or adherent monolayers, depending on different culture conditions. The neural stem cells or retinal progenitors, neurons, astrocytes, retinal ganglion cells, rod photoreceptors and the proliferating cells were evaluated with immunofluorescence analysis by Nestin or Pax6, Map2, GFAP, Thy-1, Rhodopsin and BrdU antibodies respectively. Results RPCs could propagate and differentiate in suspension or differentiation medium and express the markers of Nestin （92.86%） or Pax6 （86.75%）, Map2 （38.54%）, GFAP （20.93%）, Thy-1 （27. 66%） and Rhodopsin（13. 33%）in suspension medium; however, Nestin （60.27%）, Pax6 （52%）, Map2 （34.94%）, GFAP （38.17%）, Thy-1 （30.84%） and Rhodopsin （34.67%） in differentiation medium. 96.4 % of the population in the neurospheres was BrdU-positive cells. The cells could spontaneously adherent forming some subspheres and retinal specific cell types. Conclusion Neonatal rat RPCs possess the high degree of proliferation and can differentiate into neurons, astrocytes, retinal ganglion cells and rod photoreceptors in vitro. There are different proportions for RPCs to differentiate into specific cell types.     

公共交通行业的企业文化与制度创新

在分析企业文化内涵及作用的基础上,论述了公交行业发展要走企业文化及制度创新之路,并提出了公交行业建设特色企业文化的必经阶段.