分泌抗HSV—2型特异McAb的B_5杂交瘤细胞株的建立和初步应用

本文应用小鼠骨髓瘤细胞SP2/0—Ag14与用HSV—2 SaV株病毒免疫的BALB/C小鼠脾细胞融合,建立了一株分泌特异性抗HSV—2病毒的单克隆抗体(McAb)的杂交瘤细胞株。冻存20个月后复苏传代,仍能稳定分泌McAb。经ELISA间接法检测,该细胞培养上清滴度为1∶120,制备腹水的滴度为10~(-7),定名为B_5细胞株。B_5细胞的染色体数目为92～101,B_5McAb经鉴定属于IgG_1亚类,与HSV—2有高效价的结合反应,而与HSV-1、CMV、EHFV、Ad_3、RSV_(1387)等病毒不发生反应。应用微量细胞培养中和试验.鸡胚绒毛尿囊膜中和试验和家兔皮内感染HSV—2病毒保护试验证明,B_5McAb在试管内或机体内(兔)都能中和HSV—2病毒。B_5McAb与临床分离的19株HSV-2病毒中的14株起反应,鉴定符合率为73.6%、与5株HSV—1病毒株都不起反应。     

Managing transportation during highway reconstruction: A recommended process

Major highway reconstruction can cause significant disruptions to existing travel patterns and economic activity. Reducing these impacts on travelers, shippers, businesses and residents requires that innovative and effective transportation management actions be developed and implemented. This paper reports the major findings and recommendations of a research study on managing transportation during highway, reconstruction. The primary objectives of the study were

船舶浮态计算方法及船舶浮态图谱

本文用化非线性问题为线性问题的线性化方法,对非线性隐式的船舶浮态方程组进行了数值解,并在深入研究的基础上提出了两种形式浮态图谱,以便能迅速而准确地确定船舶各种不同装载情况下的浮态。本文给出了五万吨油轮“西湖”号算例。     

Joint development at transit stations in the United States

Joint development, as the term is generally used in connection with transit systems in the United States, is real estate development that is closely linked to public transportation services and station facilities, and takes advantage of the market and locational advantages provided by them. Research conducted by LEK Associates for the Urban Mass Transportation Administration (UMTA) suggests that, in addition to helping shape urban growth and land development, joint development is also a surprisingly effective means of increasing transit system ridership and farebox revenues, as well as a source of increasingly significant revenues from the sale or lease of air rights.The completion of nine joint development projects in as many different cities, started under the former Urban Initiatives Program, for example, net additional annual ridership might reach 12000000 one-way trips. Net additional annual farebox revenues might reach over $9000000. This added revenue would be sufficient to repay the $62 million combined UMTA/transit operator investment in the nine projects, exclusive of the costs of the basic transit system improvements around which the projects are planned, in less than six years.Among transit authority-administered joint development programs examined in a separate study, the Washington Metropolitan Area Transit Authority (WMATA) has had the most success in generating significant value capture income. For example, the cumulative revenue realized by WMATA from just six joint development projects in the Washington, DC region, through September 1983 exceeded $ 6.9 million. Projections through fiscal year 1986 indicate cumulative revenues approximating $ 28 million will be received from developer leases.     

Urban transportation modeling: The discussion continues

This article is a response to John Polak's article, entitled, A Comment on Supernak's Critique of Transport Modeling, published elsewhere in this issue of TRANSPORTATION. It offers necessary clarifications to the issues discussed in an earlier article in this journal (Supernak, 1983). It also responds to the philosophical issues discussed in Polak's article, such as the nature of transport phenomena, and the role, form and methodology of transport modeling.     

Regional impacts of new transport infrastructure: a multisectoral potentials approach

This is the second of two background papers sponsored for the Symposium by the Administrative Commission for the north of the Netherlands. Its purpose is to provide an indication of the effects of the proposed high speed rail line between Amsterdam, Groningen and Hamburg on employment in the corridor. The authors first review the techniques of forecasting these effects discussed in the literature. The potentials approach is adopted for their analysis, the mathematics and underlying assumptions of which are presented. Finally, this model is run to provide an estimate of the impact on regional employment of three variants of the proposed rail line.     

The design of bus route systems — An interactive location-allocation approach

An interactive modelling approach is developed to solve the practical problem of bus route network design. Possible bus routes are identified with facilities which can be located. Zones or pairs of zones in the urban area are identified with customers who will be allocated to the established facilities. It is shown that the classical Set Covering Problem is useful under the assumption of fixed demand; the Simple Plant Location Problem is effective under the assumption of demand which is sensitive to the level of bus service provided.     

地铁车辆段接触轨问题整治

针对深圳地铁3号线车辆段采用接触轨受电方式,存在接触轨支撑座与钢轨垫板安装距离过近而造成轨道电路红光带故障的缺陷,进行分析和研究,提出解决方案并实施,取得了较好的效果。     

土压平衡盾构在复合地层中带压进仓施工技术

结合南京地铁土压平衡盾构在复合地层中带压进仓的具体情况,通过采取带压进仓压力计算、现场试验压力确定以及实施过程,成功地解决了土压平衡盾构进行带压进仓作业,了解掘进面存在的问题以及解决方式等技术难点.通过对监测数据的对比、分析,说明该方法的可行性及合理性,为土压平衡盾构在复合地层中带压进仓的施工提供参考与借鉴.