2-氯-6-N-芳基取代嘌呤衍生物的合成

N6嘌呤衍生物是重要的核苷类药物的中间体.以2-氨基-6-氯嘌呤为原料,通过重氮化反应制得2,6-二氯嘌呤,收率30.5%.2,6-二氯嘌呤分别与苯胺、邻氯苯胺和对氯苯胺,通过N-烃基化反应制得2-氯-6-N-芳基嘌呤,收率75.2%;2-氯-6-N-2′-氯苯基嘌呤,收率68.5%和2-氯-6-N-4′-氯苯基嘌呤,收率63.2%.使用元素分析、核磁共振、质谱和红外光谱对所得产品进行分析,结果表明所得产品为目标产品.2-氯-6-N-芳基取代嘌呤衍生物合成的最佳工艺条件为：n（2,6-二氯嘌呤）∶n（苯胺衍生物）=1∶5,使用戊醇为反应中的溶剂,其用量为m（C5H11OH）∶m（2,6-二氯嘌呤）=23∶1,反应温度为80℃.     

铁路发展数字数据网的可行性分析

结合我国铁路通信网的现有发展状况，比较了国内外ＤＤＮ的发展历史，以及不同类型通信网的优缺点，对铁路ＤＤＮ建设发展的可行性进行了分析，并在此基础上对网络的拓扑结构、业务提供以及网络管理进行初步规划。     

我国商用车ABS生产和使用现状

ABS (Anti-lock BrakingSystem) 防抱制动系统是一种具有防滑、防锁死等优点的汽车安全控制系统.20世纪90年代中期以后,国际市场上的大多数车辆都装备了ABS.1999年,我国颁布了相关法规(GB 12676-1999),规定首先在重型车和大客车上安装电子控制式ABS.2004年又具体规定了必须安装的车型和时间(GB 7258-2004).时隔五年,商用车ABS及国家强制性法规实施后的现状又如何呢?     

提速对桥梁振动与车辆过桥走行性的影响及其对策

铁路虽有安全、量大、舒适、方便的优势,由于当前交通运输业竞争激烈,因此必须提高运行速度到140 km·h-1或160 km·h-1以缩短旅客在途时间.我国铁路桥梁均按120 km·h-1设计.文中主要对既有桥梁提高行车速度后桥梁振动的变化和车辆过桥时走行性及应采取的对策进行了理论和实测研究分析.     

双壳散货船结构设计体会

本文对53000吨系列双壳散货船的结构设计作简要阐述,就该系列船在设计过程中所遇到的问题及解决方法作了详细介绍。     

亚洲牛带绦虫成虫延伸因子-1基因的表达、纯化及免疫学分析

目的 对亚洲牛带绦虫成虫延伸因子-1(elongation factor 1,EF-1)基因进行克隆、表达和免疫学初步研究.方法 将亚洲牛带绦虫成虫EF-1克隆到原核表达质粒pET-28a( )中,在大肠杆菌BL-21/DE3中用异丙硫代-β-D半乳糖苷诱导表达,表达产物通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)进行鉴定,用镍离子金属螯合剂亲和层析柱进行纯化,用蛋白印迹法(Western blotting)进行免疫学分析.结果 PCR、双酶切及DNA测序结果 均表明重组质粒pET-28a( )-EF-1构建成功.重组蛋白可被感染了亚洲牛带绦虫患者血清和猪血清识别,表明其具有免疫反应性.结论 亚洲牛带绦虫成虫EF-1基因可在原核表达系统中获得具有免疫学活性的表达,为进一步研究该蛋白的功能奠定了基础.     

基于载荷谱和CAE的减振器支架优化设计

针对轻型载货车用减振器支架频繁发生断裂问题,利用Matlab软件开发减振器支架载荷谱提取程序,基于实际载荷谱输入,应用CAE分析方法进行减振器支架静强度和疲劳强度分析,发现危险位置与实际断裂位置吻合。利用上述方法进一步完成减振器支架优化设计,通过了整车可靠性试验验证,为判断减振器支架结构强度是否合格提供了有效的依据,并使减振器支架减重30%,实现了优化设计的目的。     

Design optimization of a torpedo shell structure

An optimized methodology to design a more robust torpedo shell is proposed. The method has taken into account reliability requirements and controllable and uncontrollable factors such as geometry, load, material properties, manufacturing processes, installation, etc. as well as human and environmental factors. The result is a more realistic shell design. Our reliability optimization design model was developed based on sensitivity analysis. Details of the design model are given in this paper. An example of a torpedo shell design based on this model is given and demonstrates that the method produces designs that are more effective and reliable than traditional torpedo shell designs. This method can be used for other torpedo system designs.     

Sensitivity analysis of the probabilistic damage stability regulations for RoPax vessels

In the light of the newly developed harmonised probabilistic damage stability regulations, set to come into force in 2009, this article presents a systematic and thorough analysis of the sensitivity of the Attained Subdivision Index with reference to a wide range of related design parameters. The sensitivity of the probabilistic regulations was investigated for a typical large RoPax vessel, with variation of parameters, such as the number, positioning and local optimisation of transverse bulkheads; the presence and position of longitudinal bulkheads below the main vehicle deck; the presence of side casings; and the height of the main deck and double bottom. The effects of water on deck and of operational parameters (draught, centre of gravity and trim) were also investigated. The results of the study, presented in graphical form, can provide valuable assistance to the designer when determining subdivision characteristics at the very early stage of the design process, resulting in optimal, efficient and safe ships.     

Fast direction of arrival algorithm based on vector-sensor arrays using wideband sources

An acoustic vector sensor （AVS） can capture more information than a conventional acoustic pressure sensor （APS）. As a result, more output channels are required when multiple AVS are formed into arrays, making processing the data stream computationally intense. This paper proposes a new algorithm based on the propagator method for wideband coherent sources that eliminates eigen-decomposition in order to reduce the computational burden. Data from simulations and lake trials showed that the new algorithm is valid： it resolves coherent sources, breaks left/right ambiguity, and allows inter element spacing to exceed a half-wavelength.