临床致病性不动杆菌的分子生物学鉴定

目的对临床致病性不动杆菌进行种属鉴定,并比较3种方法(44℃生长试验、特异性引物PCR扩增和16SrRNA测序)对鲍曼不动杆菌鉴定的特异性、准确性及应用价值。方法收集临床致病不动杆菌56株,首先接种在胰蛋白胨大豆肉汤中44℃进行培养,观察是否可以生长;其次,提取56株不动杆菌基因组DNA,用两组不同的特异性引物进行PCR扩增鉴定;最后,用通用引物对56株不动杆菌的16SrRNA进行测序鉴定。结果 56株不动杆菌中有17株可在44℃生长。经特异性引物PCR扩增鉴定,发现8株鲍曼不动杆菌和3株13TU型不动杆菌均可在44℃中较早较快生长。金标准16SrRNA测序确定56株不动杆菌中含有9个种,其中鲍曼不动杆菌和13TU型不动杆菌的鉴定结果与特异性引物PCR扩增法相同。结论应用分子生物学方法鉴定鲍曼不动杆菌具有准确、高效且可重复性高的优点,尤其是特异性PCR扩增法,可作为较难诊断的鲍曼不动杆菌的首选鉴定方法。     

有利于行人头保护的风挡玻璃下横梁结构优化设计与试验验证

基于某车型的风挡玻璃下横梁结构,针对提高行人头保护性能进行了优化设计.结果表明,优化后的风挡玻璃下横梁可以提供更大的变形吸能空间,降低加速度峰值和HIC值,提高行人保护性能,为车型行人保护性能评价和开发改进提供借鉴.     

Road transport and climate change: Stepping off the greenhouse gas

Transport is Australia’s third largest and second fastest growing source of greenhouse gas (GHG) emissions. The road transport sector makes up 88% of total transport emissions and the projected emissions increase from 1990 to 2020 is 64%. Achieving prospective emission reduction targets will pose major challenges for the road transport sector. This paper investigates two targets for reducing Australian road transport greenhouse gas emissions, and what they might mean for the sector: emissions in 2020 being 20% below 2000 levels; and emissions in 2050 being 80% below 2000 levels. Six ways in which emissions might be reduced to achieve these targets are considered. The analysis suggests that major behavioural and technological changes will be required to deliver significant emission reductions, with very substantial reductions in vehicle emission intensity being absolutely vital to making major inroads in road transport GHG emissions.     

Impact of information technology on the methods and working systems of the freight forwarding industry

Freight forwarding fulfils the role of information processor. The past decade has seen considerable change in the information industry. Combine these two facts and we can begin to explain why freight forwarders have a vested interest in using computer based information systems to their full. Many organizations have tried, some with considerable success, but in others a lack of real commitment and professionalism has cost considerable sums. In this paper the background to the industry is reviewed together with an analysis of the reasons for the poor implementation of successful systems.     

TBM及其在中国的应用(2)

3工程和技术实例 3.1在长大和深埋隧道工程方面其它国家的经验 3.1.1工程实例 在国际上,许多地铁、公路和水利方面的隧道正投入使用或正在建设之中,还有的才处于计划阶段.     

TBM及其在中国的应用(3)

3.6先进的掘进及支护系统 3.6.1其他先进的掘进技术 除了上述的各种主要的先进TBM技术外,还有下列几种先进的地下掘进技术:     

A combined land use-transportation model when zonal travel demand is endogenously determined

A combined transportation-land use model is proposed in this paper. Unlike other existing urban land use and transportation planning models in which a “fixed demand” for services is assumed to be known at the zonal level of an urban area, zonal travel demand is endogenously determined together with link congestion costs, optimal amounts of production and resulting efficient densities of land uses, once the transportation network is given. Some characteristics of alternative solutions are demonstrated. The proposed model represents progress over previous efforts in combining land use-transportation problems since the travel choice as to origin, destination and routes as well as amounts of goods to be produced at the optimal density of land uses are integrated into a consistent mathematical programming framework.     

Investigation of active torsion bar actuator configurations to reduce vehicle body roll

The increasing popularity of sport utility/light-duty vehicles has prompted the investigation of active roll management systems to reduce vehicle body roll. To minimize vehicle body roll and improve passenger comfort, one emerging solution is an active torsion bar control system. The validation of automotive safety systems requires analytical evaluation and laboratory testing prior to implementation on an actual vehicle. In this article, a computer simulation tool and accompanying hardware-in-the-loop test environment are presented for active torsion bar systems to study component configurations and performance limits. The numerical simulation illustrates that the hydraulic cylinder extension limits the active torsion system's ability to provide body roll angle reduction under various driving conditions. To compare the control system's time constant and body roll minimization capabilities for different hydraulic valve assemblies and equivalent hose lengths, an experimental test stand was created. For a typical hydraulic pressure and hose diameter, the equivalent hose length was not a key design variable that impacted the system response time. However, the servo-valve offered a quicker transient response and smoother steady-state behavior than the solenoid poppet actuators that may increase occupant safety and comfort.     

Recent advances in automotive technology and the cost-effectiveness of fuel economy improvement

The potential for improving the fuel economy of conventional, gasoline-powered automobiles through optimized application of recent technology advances is analyzed. Results are presented at three levels of technical certainty, ranging from technologies already in use to technologies facing technical constraints (such as emissions control problems) which might inhibit widespread use. A fleet-aggregate, engineering-economic analysis is used to estimate a range of U.S. new car fleet average fuel economy levels achievable given roughly 10 years of lead time. Technology cost estimates are compared to fuel savings in order to determine likely cost-effective levels of fuel economy, which are found to range from 39 miles per gallon to 51 miles per gallon depending on technology certainty level. The corresponding estimated increases in average new car price range from $540 to $790 (1993$). Estimated fuel savings payback times average less than 3 years and the cost of conserved energy averages $0.50 per gallon, indicating that these levels of fuel economy improvement are cost-effective over a vehicle lifetime. A vehicle stock turnover model is used to project the reductions in gasoline consumption and associated emissions that would follow if the estimated fuel economy levels are achieved. Potential trade-offs regarding vehicle performance, safety, and emissions are also discussed.