含增生结节鼠肝谷胱甘肽转硫酶的纯化和性质研究

含增生结节鼠肝GST活性在纯化过程各步均较正常肝明显增高,经DEAE-52和CM-52柱层析分离得阴离子同工酶A-1和A-2以及阳离子同工酶C-1,C-2,C-3和C-4,用等电聚焦电泳测定等电点分别为6.7,6.3,7.4,7.9,8.3和8.6。SDS-PAGE结果显示,含增生结节肝GST在正常大鼠肝GSTYa,Yb和Yc区带相应的位置,出现3条区带,各亚基分子量分别为26,000,27,000和28,600道尔顿,但活性增高明显的C-3和C-4只出现1条相当于Yc亚基的区带。含增生结节肝GST6种同工酶的带电性质及亚基组成与正常肝无明显差别。阳离子同工酶C-3和C-4活性占上柱总活性的70%,说明含增生结节肝GST活性增高以阳离子同工酶为主。     

Initial results from long-term measurements of atmospheric humidity and related parameters in the marine boundary layer at two locations in the Gulf of Mexico

Measurements of boundary layer moisture have been acquired from Rotronic MP-100 sensors deployed on two NDBC buoys in the northern Gulf of Mexico from June through November 1993. For one sensor, which was retrieved approximately 8 months after deployment, the post- and precalibrations agreed closely and fell well within WMO specifications for accuracy. The second sensor operated continuously from June 1993 to February 1997 (3.5 years). Buoy observations of relative humidity and supporting data were used to calculate specific humidity and the surface fluxes of latent and sensible heat. Specific humidities from the buoys were compared with observations of moisture obtained from nearby ship reports, and the correlations were generally high (0.7–0.9). Surface gravity wave spectra were also acquired. The time series of specific humidity and the other buoy parameters revealed three primary scales of variability, small (h), synoptic (days), and seasonal (months). The synoptic variability was clearly dominant and occurred primarily during September, October, and November. Most of the synoptic variability was due to frontal systems that dropped down into the Gulf of Mexico from the continental US followed by air masses which were cold and dry. Cross-correlation analyses of the buoy data indicated that: (1) the moisture field was highly coherent over distances of 800 km or more in the northern Gulf of Mexico; and (2) both specific humidity and air temperature served as tracers of the motion associated with propagating atmospheric disturbances. These correlation analyses also revealed that the prevailing weather systems generally entered the buoy domain from the South prior to September, but primarily from the North thereafter. Spectra of the various buoy parameters indicated strong diurnal and semidiurnal variability for barometric pressure and sea surface temperature (SST) and lesser variability for air temperature, wind speed and significant wave height. The surface fluxes of latent and sensible heat were dominated by the synoptic events which took place from September through November with the transfer of latent heat being primarily from the ocean to the atmosphere. Finally, an analysis of the surface wave observations from each buoy, which included calculations of wave age and estimates of surface roughness, indicate that major heat and moisture flux events coincide with periods of active wave growth, although the data were insufficient to identify any causal relationships.     

The Effect of Topboxes on Motorcycle Stability

Hot wire anenometry measurements have been made in the wake of a model motorcycle and rider mounted in a windtunnel. Only in tests with a topbox present is there clear evidence of eddy shedding. Calculations have been performed on the corresponding full-scale motorcycle to determine the frequencies of the weave and wobble modes. Comparison with the wake frequencies extrapolated to full size show that at speeds of 31-34 m/s they coincide with those of the wobble mode which is found to be efficiently coupled to the aerodynamic excitation.     

电气化架线防硬弯辅助支承装置

我国铁路电气化架线施工中,在传统钓“低盘高台”式架线车的作业平台上,设置的是一根小直径滚柱来支承接触线并使其转向。这是一种“接触线单点支承大转向角度”的架线技术,在一定条件下容易使接触线产生硬弯。针对这一不适应140 km/h以上时速电气化铁路建设对其接触线高平直度技术要求的问题,提出一种安装在架线车上的新型架线防硬弯辅助支承装置。     

青藏线拉萨河特大桥主桥横梁设计

拉萨河特大桥主桥采用 (3 6+ 72 + 10 8+ 72 + 3 6)m五跨三拱连续梁钢管拱组合结构体系。通过对系杆拱桥不同部位横梁受力特点进行分析 ,采取相应的设计措施 ,为系杆拱桥的横梁设计提供一种合理的设计方法。     

带功率因数补偿的单相半控桥整流器(一)

介绍了绘制正弦线电流的开关模式单相半控桥整流器,用它来获得功率因数补偿并保持直流环节电压的恒定.在该整流器中使用4个有源开关,以便在交流端电压上产生单极脉宽调制(PWM)电压波形.与中性点箝位变流器(NPC)相比,该整流器没有箝位二极管也能实现三点式PWM控制.控制电路采用两个控制回路:外部控制回路用比例积分电压控制器调整直流环节电压,采用相位闭环电路产生与电源电压同相的正弦波形来实现功率因数补偿.在内部控制回路中,用基于载波的电流控制器跟踪线电流信号.为补偿由于负载变化所引起的中点电压,控制电路采用中点电压补偿器.该整流器的交流侧可产生3个电压电平,并用计算机仿真与试验结果验证了控制算法的有效性.     

Simulated responses of the Gulf of Mexico hypoxia to variations in climate and anthropogenic nutrient loading

A mathematical model was used to simulate monthly responses of the Gulf of Mexico hypoxia to variations in climate and anthropogenic nutrient loading over a 45-year period. We examined six hypothetical future scenarios that are based on observed and projected changes in the Mississippi River discharge, Mississippi River nitrate concentrations, and ambient water temperatures. In particular, we investigated the implications of a 30% decrease in the Mississippi River nitrogen flux, which was recently proposed by the Mississippi River Watershed/Gulf of Mexico Hypoxia Task Force as a measure to reduce the size of the hypoxic zone. Model simulations suggest that the frequency of hypoxia in the northern Gulf of Mexico is highly sensitive to variations in riverine nitrate flux, but also to variations in freshwater discharge and ambient water temperatures. A 30% decrease in the Mississippi River nitrate flux, for example, would reduce the frequency of hypoxia by 37%. Nevertheless, a 20% increase the Mississippi River discharge, which may occur under some climate change scenarios, would produce an increase in the frequency of hypoxia of the same magnitude. Thus, if the potential climatic variations are taken into account, a 30% decrease in the nitrogen flux of the Mississippi River may not be sufficient to accomplish the proposed hypoxia management goal.     

评论

随釜山新港的开港，韩中两国的竞争成为当前的一大热门话题。釜山新港对成为东北亚首要物流中心这一目标志在必得，而上海洋山港对此同样成竹在胸，信心百倍。     

Generation of cyclonic eddies in the Eastern Gotland Basin of the Baltic Sea following dense water inflows: numerical experiments

A sigma (σ)-coordinate ocean model by Blumberg and Mellor (POM) is applied to study the formation processes of mesoscale cyclones observed in the Eastern Gotland Basin following the dense water inflows. The initial conditions simulate a situation when the Arkona and Bornholm basins and partially the Slupsk Furrow are already filled with the inflow water of the North Sea origin, while the Eastern Gotland and Gdansk basins still contain the old water of pre-inflow stratification. Model runs with constant and time-dependent winds, changing the buoyancy forcing, grid geometry and bottom topography display the following. Entering the Eastern Gotland Basin from the Slupsk Furrow, the bottom intrusion of saline inflow water splits in two: one goes northeast towards the Gotland Deep, and second moves southeast towards the Gulf of Gdansk. An intensive mesoscale cyclonic eddy carrying the inflow water is generated just east of the Slupsk Furrow with the inflow pulse. A number of smaller cyclones with boluses of the inflow water are formed in the permanent halocline along the saline intrusion pathway to the Gotland Deep. Following Spall and Price [J. Phys. Oceanogr. 28 (1998) 1598], the cyclones are suggested to form by the adjustment of the high potential vorticity inflow water column to a low potential vorticity environment.     

内置式永磁同步电动机无传感器速度控制和转子初始位置估计方法的应用及实验研究

针对内置式永磁同步电动机传动提出了一种新的无速度传感器控制和转子初始位置估计方法.在内置式永磁同步电动机旋转过程中,采用扩展卡尔曼滤波方法,仅需测量电机定子的电压和电流,便可得到转速和转子位置的估计值.对内置式永磁同步电动机,采用扩展卡尔曼滤波方法的主要困难在于在静止坐标系中其动态模型的复杂性.由于磁路的不对称性,在静止坐标系中建内置式永磁同步电动机的模型比建表面式永磁同步电动机模型更复杂.在无传感器的传动系统中,电机的起动过程是一个问题,因为在起动之前没有任何信息可以获得.转子初始位置估计是在电机静止时,在瞬间对定子绕组施加合适的电压脉冲序列,通过测量峰值电流获得转子位置信息.利用磁饱和效应对凸极性的影响来区分南北磁极.给出基于浮点数字信号处理器TMS320C31平台得到的内置式永磁同步电动机实验结果.