Cathepsin B antisense oligodeoxynucleotide suppresses invasive potential of MG-63 cells

Objective To study the biological effects of cathepsin B phosporotbioated antisense oligodeoxynucleotide on human osteosarcoma cell line MG-63 after transfection. Methods A 18-mer phosphorothioate antisense oligodeoxynucleotide (ASODN) targeted against the cathepsin B mRNA was transfected into the human osteosarcoma cell line MG-63 by lipofectamine 2000. The sense and nonsense oligodeoxynucleotides to cathepsin B and blank vector were used as controls. The expression of cathepsin B mRNA was examined by RT-PCR and the expression of cathepsin B was examined by Western blot. The invasive capability of MG-63 cells was evaluated by the boydern chamber assay. Results The expression of cathcpsin B was obviously inhibited in antlsense oligodeoxynucleotide treated cells compared with the control cells. The number of invading MG-63 cells was significantly lower in the ASODN-treated groups than that in the control groups. Conclusion The cathepsin B ASODN significantly inhibits the expression of cathepsin B and invasive ability of MG-63 cell in osteosarcoma.     

氧化苦参碱对慢性肾脏病患者血清MMP-9和TIMP-1的影响

目的观察氧化苦参碱对慢性肾脏病(CKD)患者血清基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶组织抑制因子-1(TIMP-1)的影响,探讨其在肾间质纤维化发生发展过程中的作用和治疗价值。方法选取健康对照者10例、慢性肾脏病患者40例,根据肾小球滤过率(GFR)分为轻度肾损害组(GFR<90 mL/min.1.73 m2,CKDⅠ)和中、重度肾损害组(GFR<60 mL/min.1.73 m2,CKDⅡ),再分层随机抽样,分为常规治疗组和氧化苦参碱组。利用双抗体夹心酶联免疫吸附法分别检测各组治疗前后血清MMP-9和TIMP-1含量,采用t检验比较两组MMP-9和TIMP-1水平的差异。结果经氧化苦参碱干预后,TIMP-1水平显著低于常规治疗组,而MMP-9水平则显著高于常规治疗组(P<0.05)。结论氧化苦参碱可下调TIMP-1水平,恢复MMP-9水平,保持MMP-9/TIMP-1平衡,减少细胞外基质(ECM)沉积,防止肾间质纤维化。     

(美)伍兹·霍尔海洋研究中心载人深潜器计划探究暨(美)新概念ALVIN号载人深潜器关键技术浅析

文章较为详细地介绍了美国伍兹霍尔海洋学研究所（WHOI）目前在建的新概念ALVIN号载人深潜器研制项目,该项目经过了美国科研机构认真详实的凋研论证而立项,新概念ALVIN号的研制对确保美国在深海研究及深海技术方面处于领先地位有着重大意义。文中介绍了新ALVIN号的主要技术性能、关键技术、建造费用、建造计划等内容。     

六轴弹性架悬高速机车牵引杆布置方式

为了开发中国运行速度为200km.h-1的2C0轴式单杆牵引高速机车转向架,研究了牵引杆布置方式对机车运行安全性的影响。采用多体动力学软件SIMPACK建立了2C0轴式弹性架悬机车整车动力学模型,在惰行、牵引和制动工况下,分析了单牵引杆由转向架向车体端部和中部牵引时,机车的直线运行性能和曲线通过性能。分析结果表明:牵引杆布置方式对机车动力学性能影响不显著,建议采用牵引杆端部牵引的方案,以简化机车车体的设计与制造。采用牵引杆端部牵引的机车可安全通过R400m和R1600m的S形曲线;通过R400m小半径曲线时,机车惰行运行的轮对横向力大于牵引和制动工况的横向力,制动工况的轮重减载率较大;通过R2800m大半径曲线时,机车牵引和惰行的轮对横向力大小相当,大于制动工况的横向力。     

A reference-dependent user equilibrium model for activity-travel scheduling

Existing user equilibrium models of activity-travel scheduling generally fall short in representing travelers’ decision-making processes. The majority have either implicitly or explicitly assumed that travelers follow the principle of utility maximization. This assumption ignores the fact that individuals may be loss–averse when making activity-travel decisions. Allowing for the situation that travelers possess accurate information of the urban-transportation system due to modern technologies, studies on reference-dependent decision-making under near-perfect information are receiving increasing attention. In view of traveler heterogeneity, individuals can be divided into multiple classes according to their reference points. In this paper, we propose a reference-dependent multi-class user equilibrium model for activity-travel scheduling, which can be reformulated as a variational inequality problem. Moreover, comparative analyses are conducted on the equilibrium states between utility-maximization (no reference) and reference-dependency of exogenous and endogenous references. A numerical example regarding combined departure-time and mode choice for commuting is conducted to illustrate the proposed model. The simulated results indicate that reference points and loss aversion attitudes have significant effects on the choice of departure time and mode.     

汽车金属部件防腐原理与措施

汽车金属部件的腐蚀是不可避免的,必须进行有效的防护。本文从金属部件腐蚀的原理入手,针对斑状腐蚀、局部腐蚀、缝隙腐蚀、受载下的腐蚀四种常见的腐蚀类型,深入分析了腐蚀的原因,然后提出了汽车金属部件防腐蚀的策略,最后系统研究了各种防腐蚀的措施,包括优化防腐设计并改善腐蚀环境、材料替换、改善涂覆和增强电化学防护等方面。通过这些分析和研究,以期达到改善现有车辆金属部件的防腐措施、提高金属部件的防腐性能的目的。     

基于Mie理论的激光雾中传输衰减特性研究

海雾是沿海地区或海上常见天气现象,对激光传输的影响很大.采用雾滴谱指数分布模型,结合能见度与含水量、模式半径的经验关系,应用Mie理论研究激光在海雾中的传输衰减.首先通过Mie衰减模型与其它衰减计算模型的比较,分析说明了其优越性;其次从波长、能见度、温度三个方面对激光衰减的影响作用进行了仿真分析,并得出了海雾衰减的相关结论;最后总结了Mie衰减模型的主要误差来源,提出一种提高模型计算精度的方法,并对其可行性进行了分析.     

双曲抛物面薄壳屋盖的制作与设计

为了方便双曲抛物面薄壳屋盖的制作,采用数学建模的方法对双曲抛物面薄壳屋盖进行理论计算,得到了直母线钢筋数以及直母线钢筋在空间的位置、锚固面方程以及与直母线交点、直母线之间的交点和直母线与锚固面的法线之间夹角的数学表达式。利用Matlab软件经过实例计算验证了其有效性,为今后双曲薄壳屋盖的制作提供了理论依据。     

基于邻域极值数的协同粒子群优化算法

提出了一种基于邻域极值数的协同粒子群优化算法。该算法将种群分为若干个独立进化的子种群。根据邻域极值数确定各子种群的生存状态。根据子种群的生存状态对子种群实施相应的控制操作,提高子种群的搜索能力,实现子种群之间的信息共享,共同进化。测试结果表明基于邻域极值数的协同粒子群优化算法是一种高效稳健的全局优化算法。     

基于转速波动控制的整车启动抖动优化

对某三缸发动机车型存在整车启动抖动大的问题进行分析,发现启动抖动在转速波动上升过程中产生,与转速波动的大小存在相关性.通过研究起动机、燃烧激励、双质量飞轮等因素对启动工况下转速波动的影响,分析其与整车启动抖动大小的相关性,并通过提高起动机启动转速、降低燃烧激励所产生的缸压及降低双质量飞轮第二惯量等对策,降低了转速波动,...