论经济新常态下交通运输行政执法体制改革

交通运输是国民经济基础性行业,经济新常态下改革交通运输执法体制是贯彻中央全面深化改革、全面依法治国方针的内在要求,是消除执法体制机制弊端、根治执法乱象、破解交通运输发展瓶颈的有效途径,改革的主要内容和目的主要有整合职能、精简机构、核定编制、保障经费四个方面。改革中市辖区不设置执法机构,市管公路治超站移交县级管理,妥善进行富余人员转岗分流,要制定配套政策、采取措施保障改革顺利进行,处理好改革与稳定的关系,做到改革与治理两不误。     

基于下游大水位变率的下水式垂直升船机随动技术的设计与实现

下游航道水位变率大,快速适应水位变化的准确停位随动技术,对下水式升船机的传动控制很重要.本文介绍下水式垂直升船机随动控制技术.首先,对控制对象的特性进行分析,描述了系统的速度运行曲线,对位置控制功能进行了研究.然后,搭建试验平台对本控制方案的性能进行了测试,给出了测试的方法和试验结果.实验结果表明本控制系统具有定位精度高、响应速度快等优点,能够适应下游大水位变化率的下水式垂直升船机准确停位要求.     

汽车电气系统寄生电流的检测与控制

汽车在日常使用和维修中能使电子元件、控制模块性能会下降,线路的各个插接件之间会产生松动等。这都能使汽车电气系统的寄生电流增加,造成蓄电池损坏和火灾的危险性。所以经常检测电气系统的寄生电流尤为重要。     

A proposed vision and vehicle-to-infrastructure communication-based vehicle positioning approach

It is essential to obtain accurate location of vehicles for new applications of Intelligent Transportation Systems. To remedy the defects of present Global Positioning System and vehicle-to-infrastructure (V-I) positioning technology, a new positioning approach based on vision and V-I communication is proposed. This approach aims at lane-level positioning with lower cost than conventional ones. In this approach, the position of the vehicle is represented by its lateral position (the lane number) and longitudinal position (the distance from entrance of the road) in a course coordinate system along the road; the specific lane the vehicle is occupying (the lane number) can be judged using the information of lane lines detected by vision systems; then the distance to the vehicle is obtained by a Road Side Unit (RSU) during the V-I communication; and the longitudinal position is calculated. The error of the approach on typical operating conditions is analyzed, indicating that the new approach can achieve the accuracy of less than 0.31 m for straight road and 0.58 m for typical arc road with ultra-wideband communication and ranging technologies and rational arrangement of RSUs. The feasibility of this approach is presented.     

Approximate optimal AUTOSAR software components deploying approach for automotive E/E system

The AUTOSAR has been developed as the worldwide standard for automotive E/E software systems, making the electronic components of different suppliers to be employed universally. However, as the number of component-based applications in modern automotive embedded systems grows rapidly and the hardware topology becomes increasingly complex, deploying such large number of components in automotive distributed system in manual way is over-dependent on experience of engineers which in turn is time consuming. Furthermore, the resource limitation and scheduling analysis make the problems more complex for developers to find out an approximate optimal deploying approach in system integration. In this paper, we propose a novel method to deploy the AUTOSAR components onto ECUs with the following features. First, a clustering algorithm is designed for deploying components automatically within relatively low time complexity. Second, a fitness function is designed to balance the ECUs load. The goal of our approach is to minimize the communication cost over all the runnable entities while meeting all corresponding timing constraints and balancing all the ECUs load. The experiment results show that our approach is efficient and has well performance by comparing with other existing methods in specific and synthetic data set.     

Apoptin原核表达载体的构建及在大肠杆菌中的表达

目的构建Apoptin的原核表达载体,并制备抗原物质Apoptin融合蛋白。方法在获得Apoptin融合基因的基础上,成功构建了Apoptin的高效原核表达载体pET-28a( )-Apoptin,将该质粒转化至大肠杆菌E.coliBL21(DE3)受体菌中,以IPTG对其进行诱导表达,聚丙烯酰胺凝胶电泳分析目的蛋白。结果转化有Apoptin的原核表达载体pET-28a( )-Apoptin的大肠杆菌E.coliBL21(DE3)经IPTG诱导后,经SDS-PAGE分析,在相对分子质量约17 000的位置出现目的蛋白条带,大小与Apoptin融合蛋白一致。结论Apoptin原核表达载体pET-28a( )-Apoptin能够表达出Apoptin融合蛋白,为进一步的Apoptin研究和制备Apoptin抗体奠定了基础。     

幽门螺杆菌尿素通道蛋白基因UreI载体的构建、融合表达及纯化

目的构建幽门螺杆菌(H.pylori,Hp)尿素通道蛋白基因UreI的融合表达载体,并在E.coliBL21中表达,为进一步研究UreI的功能奠定基础。方法利用分子克隆技术以Hp DNA染色体为模板,扩增Hp UreI基因片段,将目的基因UreI与载体pET32a( )分别经kpnⅠ和HindⅢ双酶切,纯化,连接。筛选阳性重组载体,转化大肠杆菌BL21,以IPTG诱导表达pET32a( )/UreI融合蛋白。表达产物经Ni-NTA琼脂糖树脂纯化后,用SDS-PAGE及Western blot分析。结果经酶切、测序分析表明,插入的基因片段为Hp UreI编码基因,由585 bp组成,与GenBank相应菌株HP AM417 609序列完全一致,无碱基突变。经SDS-PAGE分析显示重组质粒pET32a( )/UreI在原核细胞中得到了高效融合表达,其重组融合蛋白分子量为130 ku,经Ni-NTA琼脂糖树脂纯化后,其纯度达95%以上,Western blot分析显示,该重组融合蛋白可被6-His鼠抗单克隆抗体所识别,具有良好的反应原性。结论成功地克隆和构建了原核表达载体pET32a( )/UreI,并在原核细胞中得到了高效融合表达。     

液压驱动转盘在海洋固定平台模块钻机上的设计与应用

海洋固定平台模块钻机空间狭小、布局紧凑、要求设备具有尺寸小、质轻、集成度高、运行安全可靠等特点,而传统电机驱动转盘的驱动方式存在传动链长、传动部件多、传动装置复杂、结构尺寸庞大等问题,在模块钻机尺寸有特殊要求的情况下安装难度大、使用和维修不便,影响总体布局和结构形式的设计。针对上述情况,通过液压驱动转盘与电驱动转盘进行对比,总结出液压驱动转盘小巧轻便、易于建造安装、能够优化钻台结构、操作维护费用低、安全可靠等特点,可为空间有特殊要求的海洋固定平台模块钻机提供一定的参考。     

基于模糊物元的沿海港口物流综合服务水平评价与应用

港口作为全球供应链的中心节点,正在以各种方式参与到物流服务中,大力发展港口物流已经成为我国许多沿海港口提高综合竞争力的首要选择。分析了港口物流综合服务水平的内涵,提出了港口物流综合服务水平的构成框架,建立了基于模糊物元的沿海港口物流综合服务水平评价指标体系与模型,对我国大陆沿海典型港口的物流综合服务水平进行评价和比较的实证研究。实证算例分析结果表明,应用模糊物元理论进行沿海港口物流综合服务水平评价是十分有效的,该方法计算比较简便,论证结论客观、合理,对促进我国大陆沿海港口物流服务环境的建设有重要借鉴意义。     

某型号制导电子箱的检测系统设计*

为解决现有导弹检测设备存在的缺陷,设计了一种模块化、通用化、智能化和标准化的综合检测修理平台。论文介绍了系统的硬件组成以及基于Labwindows／CVI的软件开发。通过部队实践证明,该系统具有重大军事效益和经济效益和广泛的运用前景。