Fine Mapping of a Deafness Mutation hml on Mouse Chromosome 10

Objective To map a mouse deafness gene.identify the underlying mutation and develop a mouse model for human deafncss.Methods Genetic lindage cross and genome scan wer used to map a novel mutation named hypoplasia of the membranous labyrinth (hml),which causes hearing loss in mutant mice.Results ① hml was mapped on mouse Chr 10 (～43 cM from the centromere) suggests that the homologous human gene is on 12q22--q24, which was defined on the basis of known mouse-human homologies (OMIM,2004). ② This study has generated 25 polymorphic microsateUite markers, placed 3 known human genes in the correct order in a high-resolution mouse map and narrowed the hml candidate gene region to a 500 kb area.     

EFFECTIVE SHIELDING OF UNIDIRECTIONAL CURRENT GENERATED MAGNETIC FIELDS

Introduction　　 Environmentalelectromagnetic fields are ofin-creasing concern.Passive shielding is often em-ployed to reduce magnetic fields. Cylindricalshielding geometry is widely employed in powerin-dustry such as protective pipes,cable ducts andlightning down- conductors. Although shielding ef-fects of such conducting shells on magnetic fieldshave been recognized in recent decades,most ofthe studies have been devoted to the case of“dipole” ( opposite equal currents) sources or bal-anced…     

国家级精品课程的建设与实践

结合"船舶内燃机使用及维修"国家级精品课程建设的实践,分析了高职精品课程的定位和特点,介绍了高职精品课程建设中教学内容、实践教学环节、教材、教学方法和手段、师资队伍等方面的实施。     

我们实行的行车安全管理措施

我公司结合实际,通过分析研究,部署了压减交通违法行为的九项措施,实行以来确保了行车安全形势的稳定.     

牛黄清心丸中砷的研究

临床常用的牛黄清心丸因含有雄黄致使砷含量增高，为研究砷的有关毒性问题，就此进行了牛黄清心丸的物相分析、药代动力学和脏器残留量的研究。     

榄香烯联合三苯氧胺对乳腺癌MCF-7细胞株的抑制效应

目的观察榄香烯和三苯氧胺对乳腺癌细胞的抑制效应,尤其是两者联合应用有无协同增效作用。方法将MCF-7细胞培养于RPMI1640培养基中,置入37℃,100%湿度,5%(体积分数)的CO2培养箱内。接种细胞并加入实验药物,用CCK-8法检测三苯氧胺联合榄香烯对细胞的增殖抑制效应,用酶标仪在450nm测量每孔吸光度值,计算每组细胞抑制率。结果无毒剂量的榄香烯与治疗剂量的三苯氧胺联合应用,吸光度均值较单用三苯氧胺明显下降,并且相应的细胞抑制效应较单用组明显上升。结论无毒剂量的榄香烯能够使三苯氧胺对乳腺癌细胞的抑制效应显著增强,提示榄香烯与三苯氧胺联合应用具有协同作用。     

浅谈高速公路养护机械的现状与发展

由于国民经济飞速发展带来的交通流量迅猛增长、重载车辆的增多,特别是普遍存在的严重超载现象,导致近年来修筑的高速公路早期损伤的出现频繁,这就在客观上给我国高速公路的养护工作增加了压力。     

An Intelligent Master Model of Computer Aided Process Planning for Large Complicated Stampings

Process planning for large complicated stampings is more complicated, illegible and multiform than that for common stampings. In this paper, an intelligent master model of computer aided process planning （CAPP） for large complicated stampings has been developed based on knowledge based engineering （KBE） and feature technology. This innovative model consists of knowledge base （KB）, process control structure （PCS）, process information model （PIM）, multidisciplinary design optimization （MDO）, model link environment （MLE） and simulation engine （SE）, to realize process planning, optimization, simulation and management integrated to complete intelligent CAPP system. In this model, KBE provides knowledge base, open architecture and knowledge reuse ability to deal with the multi-domain and multi-expression of process knowledge, and forms an integrated environment. With PIM, all the knowledge consisting of objects, constraints, cxtmricncc and decision-makings is carried by object-oriented method dynamically for knowledge-reasoning. PCS makes dynamical knowledge modified and updated timely and accordingly. MLE provides scv. cral methods to make CAPP sysmm associated and integrated. SE provides a programmable mechanism to interpret simulation course and result. Meanwhile, collaborative optimization, one method of MDO, is imported to deal with the optimization distributed for multiple purposes. All these make CAPP sysmm integrated and open to other systems, such as dic design and manufacturing system.     

液相色谱-离子阱质谱联用法测定西洋参药材中p-F_(11)含量

目的　建立液相色谱 离子阱质谱联用法 ,测定西洋参特有指标成分拟人参皂苷 p F11含量。方法　通过固相萃取小柱预处理样品 ,电喷雾质谱正离子模式全扫描法检测 ,选择离子法定量 ,用液相色谱 蒸发光散射法对该定量方法进行了佐证。结果　线性范围为 0 .4 5～ 7.2 0 μg ,回收率为 98.7% ,精密度和重现性实验RSD值分别为 2 .5 %和2 .8% ,样品在 4 8h内稳定性良好。该方法测得 p F11质量浓度为 3.0 1mg·g-1。结论　该方法有良好的精密度和重现性 ,能准确分析、测定西洋参药材中 p F11含量     

Ⅰ型胶原及NF-κB与动脉粥样硬化关系的实验研究

目的探讨动脉粥样硬化(AS)时血脂成分的改变以及Ⅰ型胶原mRNA与其蛋白、NF-κBmRNA与其蛋白在兔AS发生中的作用及其机制,为临床预防及治疗AS斑块提供新的理论依据。方法采用HE、免疫组化及原位杂交法染色,光镜观察AS病变,并应用CMIAS真彩色医学图像分析系统分析;检测40只食饵性AS新西兰兔血脂成分的改变及血管壁的病理变化、Ⅰ型胶原mRNA与其蛋白、NF-κBmRNA与其蛋白的表达情况。结果实验兔15周时血胆固醇(TC)升高约43倍,血低密度脂蛋白(LDL)升高约37倍。主动脉内壁可见脂质条纹形成。主动脉AS病变Ⅰ型胶原、NF-κB蛋白及Ⅰ型胶原mRNA、NF-κBmRNA表达定量(A值)分别为0.27±0.02、0.19±0.01及0.30±0.03、0.35±0.03,显著高于对照组(0.08±0.01、0.09±0.01及0.11±0.01、0.10±0.09,P<0.05)。结论AS时Ⅰ型胶原增多刺激NF-κB的活性,可能参与了血管AS的形成。