智能交通系统及其车辆自动控制技术

本文简要介绍了智能交通系统的概念、发展状况、基本内容和研究开发过程，进一步阐述了智能交通系统中的先进车辆控制技术。综述了汽车纵向控制、横向控制、自动驾驶及车列控制。最后，对我国如何发展智能交通系统和先进车辆自动控制技术进行了探讨。     

最小二乘平差在纸海图矢量化过程中的应用

在矢量化纸海图时,由于基准点的采样误差,导致转换参数的误差,最终导致电子海图的空间对象的精度不高。本文应用最小二乘法对基准点的采样误差实施平差,提高转换参数的精度,最终可较大幅度地提高电子海图中空间对象的精度     

Identity-based threshold decryption on access structure

For the applied limitation of the existing threshold decryption schemes based on the (t, n) structure, an identity-based threshold decryption scheme which can be applied on the access structure is proposed through designing a special distribution algorithm of the private key shares. The generation and distribution of private key shares, the encryption, the decryption and the combination are introduced in detail. The validity and security of the scheme are proved and analyzed. Comparisons with the existing schemes show that the proposed scheme is more flexible.     

防火墙技术的探讨

介绍了防火墙的概念、原理、分类及比较,指出了传统防火墙存在的不足之处,提出了一种新型防火墙系统——嵌入式防火墙系统,并对防火墙的发展作了前景展望。     

Non-main Stream Plays Main Role in Shipbuilding Market

Comparing with both the major shipyards and shipping companies that were facing an market decline and hard time,the non-mainstream ship types,non-mainstream ship manufacturers and non-mainstream ship owners,as main roles,made a wonderful show of living and developing in 2009.     

梁板式高桩码头三维数值计算分析

针对现行的高桩梁板式码头平面设计方法的不足,提出利用Ansys有限元软件对梁板式高桩码头进行三维有限元计算与分析.通过后处理能够得到码头各部分的位移、应力、应变、剪力及弯矩的详细数据,计算结果更符合工程实际,为以后梁板式高桩码头的结构设计校核提供了参考依据.     

认证认可：中国影响世界

国际认可论坛（IAF）和国际实验室认可合作组织（ILAC）是国际认证认可界的两大权威组织。今年是ILAC多边互认协议签署10周年和IAF多边互认协议签署12周年。10月28日,IAF/ILAC联合年会在上海举行。在此次由中国合格评定国家认可委员会（CNAS）承办的年会上,来自73个国家和经济体的IAF/ILAC成员代表,以及相关国际组织的代表200多人共同分享了IAF/ILAC多边互认协议庆祝活动。在年会上举行多边互认协议庆祝仪式,这是IAF/ILAC历史上的第一次。业内人士认为,在中国举行的此次年会,是国际认证认可组织对中国的重视和信任,充分凸显了中国认证认可在世界的影响力和地位。     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.     

加强配送网络建设做好到达配送作业

分析了中铁快运公司到达配送作业的基本情况．提出了存在的主要问题，阐述了做好到达配送工作的思路。