Direct resolution of the pitch of DNA on positively charged lipid bilayers by frequency-modulation AFM

High resolution structural studies of DNA and DNA binding proteins by atomic force microscopy (AFM) require well-bound samples on suitably flat substrates. Adsorbing the DNA onto a positively charged supported lipid bilayer has previously been shown to be a potentially effective strategy for structural studies with AFM. Here, using our home-built frequency-modulation AFM (FM-AFM), we show that these bilayer substrates are only maximally effective for high resolution AFM when the samples are short, linear DNA, compared with circular plasmid DNA. We find that, with the former sample, the measured width of the DNA is about 2 nm, the known DNA diameter, and there is a clear height modulation along the length of the DNA with a periodicity of about 3.4 nm, in excellent agreement with the known pitch of the double helix. This sample preparation strategy is expected to enable higher resolution studies of DNA and DNA binding proteins with FM-AFM than that can presently be achieved.     

机场道面钻芯劈裂强度的尺寸效应系数

通过对比相同配合比下的室内标准模制圆柱体混凝土试样与现场试验板钻芯标准芯样之间的劈裂强度,探讨了钻芯取样测试对劈裂强度的影响;通过对不同长度芯样的劈裂强度进行对比,研究了试样长度对芯样劈裂强度的影响规律。结果表明:由于钻芯过程产生的损伤及试验板与模制圆柱体试件的混凝土拌和、振捣、养护条件不同,导致钻芯法测得的混凝土劈裂强度相比室内试验降低15.7%;另外由于圆柱体试件的长度增加,混凝土试件的受力平面也随之增大,平面内存在的微裂缝、微孔洞的数量也会随之增多,导致随着试样长度的减小,测得的试样劈裂强度逐渐增大。最终得出国内某通用机场新建飞行区工程的劈裂强度与长度的修正系数。     

结合兰州彭家坪T210#路建设浅淡城市高坪地区道路选线

城市高坪地区通常存在区域交通不畅的问题,出口道路经常制约着城市高坪地区的经济、文化发展.该文结合兰州市彭家坪T210#路路线选择,总结了城市面上高坪地区选线的几点经验,可供道路专业人员参考.     

Further study on the ultimate strength analysis of spherical pressure hulls

From a comparative study it is concluded that current design rules for the spherical pressure hull of manned submersible need to be updated and unified. In this paper, a series of spherical pressure hulls are calculated by nonlinear FEM. Based on these numerical results, the influence of critical arch length and the relationship of ultimate strength with t/R and structural imperfection are studied. Finally, empirical formulae for the ultimate strength of the titanium alloy spherical pressure hull of deep manned submersible based on numerical computations are given. The formulae can be used as the core equations to update current design rules.     

橡胶球形关节压装装置的设计与应用

介绍了DF11型内燃机车轮对橡胶关节压装装置的工作原理、主要结构、关键部件的设计及应用情况。     

余杭水路运输应对运河综合治理与保护的发展策略

详细分析了余杭区水运发展现状,指出应充分利用自身的区位优势,紧紧抓住机遇,制定科学合理的规划和措施,更好地应对运河综合治理与保护,真正发挥运河“黄金水道”的价值。     

大跨度软岩公路隧道控制爆破技术

介绍凉风凹1#隧道进口段光面爆破的特点及爆破方案的确定,进而阐述爆破参数的选择、施工方法及工艺对控制隧道超欠挖起的作用,该工程开挖段光爆效果良好。     

视频压缩技术的发展方向

MPEG的发展历史、MPEG-1、MPEG-2及MPEG-4标准的特点，并对MPEG的发展方向提出了自己的观点。     

大型船舶航迹多变量随机最优控制

基于随机控制理论，研究了大型船舶航迹的随机最优控制。以Mariner号船舶为研究对象，建立了该船的运动方程和干扰模型，在此基础上，利用卡尔曼滤波器对航向角Ψ、舵角δ、船位信息(经纬度)等进行最优估计，采用LQG随机控制方法对航迹进行随机最优控制。仿真结果表明航迹控制性能良好，能精确保持航迹。     

电子衡器中的零点跟踪装置

零点跟踪装置在电子衡器中是一种很重要的功能,目前,由于部分检定人员对其原理不了解,在检定过程中出现一些问题。为此,对JJG555-96《非自动称通用检定规程进行介绍。