Novel experimental strategy for high resolution AFM imaging of membrane-associated bacterial toxins

Bacterial pore-forming toxins （PFTs） are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy （AFM） has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O （PFO）, as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution.     

基于PLC与变频器的船用载货电梯控制系统研究

某型船用载货电梯采用PLC控制。PLC单独使用时,在控制装置在使用过程中存在一些不足,会出现运行不稳定、能耗大、噪音大等问题。通过加装变频器对某型船的PLC单独控制的系统进行系统的升级改造,介绍了基于PLC与变频器的电梯控制系统硬件组成方案及逻辑控制程序。仿真结果表明加装变频器后,电梯故障率降低,提高了电梯运行的安全性和可靠性。     

A non-geometrically similar model for predicting the wake field of full-scale ships

The scale effect leads to large discrepancies between the wake fields of model-scale and actual ships, and causes differences in cavitation performance and exciting forces tests in predicting the performance of actual ships. Therefore, when test data from ship models are directly applied to predict the performance of actual ships, test results must be subjected to empirical corrections. This study proposes a method for the reverse design of the hull model. Compared to a geometrically similar hull model, the wake field generated by the modified model is closer to that of an actual ship. A non- geometrically similar model of a Korean Research Institute of Ship and Ocean Engineering (KRISO)’s container ship (KCS) was designed. Numerical simulations were performed using this model, and its results were compared with full-scale calculation results. The deformation method of getting the wake field of full-scale ships by the non-geometrically similar model is applied to the KCS successfully.     

汽车雨水管理的数值仿真与评价

汽车在雨天行驶时,落到侧窗表面的雨水会影响驾驶员的侧向视野和后视野,从而危及行车安全,故侧窗雨水管理对于整车安全非常重要。但迄今为止对于侧窗雨水管理问题尚未有一个统一而有效的评价方法。为此,本文中应用液膜模型和拉格朗日粒子模型对某款SUV进行了雨水仿真,基于人机工程学和粒子束轨迹技术提出了侧窗雨水管理的评价方法,并对仿真结果进行了相应的分析。     

浅析K型隐藏式把手受力件材料应用及DV测试

随着近年来汽车创新发展不断兴起,汽车把手这一传统领域也推出了创新产品隐藏式车门把手.针对目前市场出现旋转式隐藏门把手断裂或折断等失效问题,提出了对于隐藏式把手总成主要受力件的塑料材料选择方案和相关塑料材料物理性能分析.又对此方案下的总成试验件进行了机械性能测试并给出结果.     

海浪与船舶安全航行

本文采用流体重力波动力学原理,分析因流体重力波引起的海面动能的传播,即海水表面质点的扰动,对航行在大风浪的船舶有一定的影响,为了使船舶在大风浪中安全、经济航行,航海人员必须了解大风浪的发生、发展和传播,并采取有效、合理的措施,保证船舶在大风浪中安全、经济航行。     

亚洲豪华游船市场发展研究

亚洲经济的发展促进了豪华游船市场的开发及发展,为此论述了亚洲豪华游船发展现状及发展原因,并对中国、韩国及日本之间豪华游船市场发展进行了展望。     

船用柴油机12PA6B超负荷性能试验研究

我国船用柴油机功率标准规定柴油机应能够在超负荷功率(即110%标定功率)下连续运转。超负荷工况各参数变化可以反映柴油机的整体性能,是船用柴油机负荷试验的重要工况点。本文通过陕柴重工生产的12PA6B柴油机的负荷试验,研究证实该机型在超负荷工况下能保持较低的燃油消耗率和烟度排放,以及较高的功率和扭矩输出。     

基于Halcon及VS的动车组制动闸片厚度自动识别模块

研究基于图像处理和模式识别的动车组闸片厚度自动识别模块,解决动车组制动时因闸片过薄而导致的车轮迅速升温,甚至引发不安全状态的问题.该模块可实现动车组通过时自动检测,即自动拼图、图像预处理、模型定位及制动闸片厚度计算,并对厚度小于一定数值的闸片进行自动报警.通过大量实验和测试表明,该模块可以有效地计算闸片的厚度,具有很好...     

基于抖音平台开展汽车营销课程改革与探索

2020年,在新冠疫情全球持续蔓延的背景下,面对汽车销量近年的持续下滑,汽车厂家和经销商都积极地在互联网与新媒体上进行各种汽车营销活动。为了使职业院校汽车营销专业的课程内容与企业需求更为贴合,文章以抖音短视频平台中的汽车营销模式为例,探索汽车营销的课程改革,为职业院校汽车营销专业的师生提供借鉴。