生存素在5-氟尿嘧啶诱导人绒癌细胞株JAR凋亡中的作用

目的探讨生存素(survivin)在化疗药物5-氟尿嘧啶(5-Fu)诱导的人绒癌细胞株(JAR)凋亡中的作用。方法用JAR细胞株进行培养传代,以不同浓度5-Fu作用于JAR细胞,MTT方法观察5-Fu对JAR细胞生长的抑制情况,流式细胞仪检测细胞凋亡率,用逆转录聚合酶链(RT-PCR)和免疫印迹技术(Western-blot)方法检测survivin基因和蛋白的表达。结果①5-Fu对JAR细胞的生长具有抑制作用,并有浓度和时间的依赖性;②5-Fu诱导JAR细胞凋亡,凋亡率随浓度而增加;③5-Fu作用后survivin基因和蛋白表达减少,随浓度增加而减少。结论5-Fu可能是通过降低survivin表达水平,诱导JAR细胞凋亡。     

The changes of neuronal activity of the zone incerta in a rodent model of Parkinson＇s disease

Objective To study the changes in neuronal activity of the zone incerta (ZI) following the unilateral lesion of the nigrostriatal pathway. Methods Eiectrophysiological recordings of ZI neurons were made in normal rats and in two groups of rats at different time intervals after injection of 6-hydrodopamine (6-OHDA) into the pars compacta of snbstantia nigra by extracellular recording in vivo. Results The results showed a significant increase in the mean firing rate of ZI during the second and fourth weeks after 6-OHDA lesion [-n=32, (3.6±2. 2)Hz, P<0.001; n=35, (9.3±6.6)Hz, P<0. 001, respectively] compared to that of normal rats [n=39, (9.2±5.2)Hz]. However, no significant change was observed between two groups of 6-OHDA-lesioned rats. With regard to firing pattern, 7.7%(3/39) of ZI neurons discharged regularly, 82.1% (32/39) irregularly and 10. 3% (4/39) in bursts in normal rats. During the second week after 6-OHDA lesion, the regular, irregular and bursting firing neurons recorded in the ZI were 9.4% (3/32), 59. 4% (19/32) and 31.3% (10/32), and during fourth week, the regular, irregular and bursting firing neurons were 14.3% (5/35), 57.1% (20/35) and 28. 6% (10/35), respectively. The firing pattern of the neurons in the three different groups did not change significantly. Conclusion These results suggest that the firing rate of ZI neurons in 6-OHDA-lesioned rats is increased significantly, which may contribute to the pathophysiology of Parkinson's disease.     

Effect of ABCA1-V771M polymorphism on plasma lipid levels and its relationship with coronary atherosclerotic heart disease

Objective To explore the risk association of ABCA1-V771M polymorphism with coronary heart disease (CHD) in Hart nationality in Northwest of China. Methods With case-control study, ABCA1-V771M polymorphism was detected in 204 unrelated Hart nationality people in Northwest of China, and all the subjects by coronary angiography were grouped into 106 cases and 98 controls. The genotypes and alleles frequency distribution of ABCA1-V771M polymorphisms were analyzed by PCR-RFLP analysis, and the clinical statistics of serum lipids were compared and its effects of ABCA1-V771M polymorphism on the plasma lipid levels and coronary atherosclerotic heart disease were analyzed. Results The genotypic frequencies of ABCA1-V771M polymorphism matched well under Hardy-Weinberg equilibrium (P>0.05), V and M allelic frequencies were 33.3% and 66.7%. In comparison with VV VM genotype carriers, MM genotypes carriers had much lower plasma levels of HDL-C (P<0. 001) and much higher plasma levels of TG (P<0. 05). M allelic frequency in CHD group was significantly higher than V allelic frequency (P<0. 05). M allele was related with more severity of atherosclerosis in the coronary artery than V allele (P<0.05). However, there was no obvious difference in the incidence of AMI among carriers with three genotypes of ABCA1-V771M polymorphism (P>0.05). Conclusion ABCA1-V771M polymorphism was not only associated with the plasma levels of HDL-C and TG, but also related to the susceptibility and severity of coronary atheroselerotic heart disease. Moreover, M771 allele appeared to be atherogenie among Han population in Northwest of China.     

应用离心造粒法制备盐酸二甲双胍微丸的研究

目的研究离心造粒粉末层积法制备盐酸二甲双胍微丸。方法采用多功能微丸包衣造粒机制备微晶纤维素空白丸芯和盐酸二甲双胍微丸,并且通过产率、粒径和粒径分布、堆密度、表面形态和圆整度、脆碎度和释放度对盐酸二甲双胍微丸进行质量评价。结果以盐酸二甲双胍∶乳糖∶微晶纤维素=100∶5∶3的比例,主机转速150 r/min,喷浆泵转速20 r/min,供粉速度25 r/min,滚圆6 min,进行制备微丸,获得了很好的效果。结论所制备的盐酸二甲双胍微丸圆整度好,粒径均匀,硬度合格,流动性好,释放完全,无骨架结构。     

动力总成悬置支架多目标拓扑优化和强度分析

基于Optistruct,以支架的最小柔度、最大固有频率作为优化目标函数;以应力、体积作为约束函数,对一发动机后拉杆安装支架进行拓扑优化,优化结果表明:在满足悬置支架强度和模态要求下,质量减少了12%。     

自锚式悬索桥主缆无应力长度计算

主缆无应力长度的计算,是悬索桥施工监控中的重要内容。利用Midas/civil对松原天河北汊桥建立有限元模型,考虑主索鞍和散索鞍处圆曲线影响,对主缆无应力长度进行修正。由于主缆从边跨至中跨通过鞍槽进行了竖向和横向两次转向,因此需要对竖向和横向主缆的无应力长度进行修正,为计算空间索面自锚式悬索桥主缆无应力长度提供参考。     

吉林兰旗松花江特大桥索塔锚固区空间应力分析

吉林兰旗松花江特大桥是一座单索面双塔预应力混凝土斜拉桥,索塔锚固区采用U形预应力钢束锚固形式。通过运用现代有限元方法,对该桥的索塔锚固区进行了空间应力分析,论证了该桥的索塔锚固区设计是安全的;同时也探讨了在强大斜拉索力作用下环向预应力锚固形式的受力特点,为同类型桥梁的索塔设计提供了重要的参考依据。     

环氧沥青混凝土的水损坏机理研究

利用冻融循环前后环氧沥青力学强度、吸水率、p H值以及DMA分析,并结合环氧沥青混凝土剥离断面的显微照片、环氧沥青的荧光显微照片,研究了环氧沥青混凝土在冻融循环实验条件下的水损坏机理。研究表明:环氧沥青混凝土在冻融循环条件下的水损破坏主要表现为,物理方面沥青相与环氧树脂以及集料分散情况的破坏,化学方面环氧树脂固化结构的破坏,即在冻融循环初期,水分子进入环氧沥青体系中造成环氧树脂发生水解,破坏环氧树脂的交联固化的分子结构;冻融循环后期,水分子使沥青相从环氧树脂固化系中逐渐析出,沥青逐渐析出固化体系,导致体系的分散结构,造成体系拉伸强度从5.12 MPa降低到3.46 MPa,降幅约32.42%,黏结强度从47.87 MPa降低到46.31 MPa,降幅为3.25%。     

基于改进GA优化的水下机器人航向鲁棒控制

基于某型号水下机器人水平面线性化模型,针对水下机器人系统存在的多种不确定因素,工作环境复杂多变的问题,采用混合灵敏度法设计水下机器人航向鲁棒控制器。在设计中,通过罚函数小生境遗传适应度值作为桥梁建立起遗传寻优与混合灵敏度参数之间的关系,构成多目标优化问题寻优加权函数。实验结果表明,所用方法可在保证系统较强鲁棒性的同时,又能兼顾时域指标和混合灵敏度加权函数频域要求,获得综合性能指标最优的鲁棒控制器,控制效果较好。     

锦州龙栖湾港区规划水动力泥沙数学模型研究

根据实测水文资料建立锦州龙栖湾港区工程附近海域的潮流泥沙数学模型,对3个规划方案共6种工况进行研究。研究成果表明,对部分海域进行围填,局部流场变化较大,但流速变化超过0.1 m/s的范围较小。各方案航道内淤强差别较大,不同规划方案港池及航道年淤积量在114万耀297万m3之间。综合考虑认为规划方案三更适合港区发展需要。