Study on T lymphocyte subsets and NK cells in patients with Graves＇ disease combined with type 2 diabetes

Objective To investigate changes in T lymphocyte subsets and NK cells in patients with simple Graves' disease (GD)and Graves' disease combined with type 2 diabetes mellitus (GD/T2DM). Methods Fifteen cases of GD/ T2DM were selected from our hospital from November 2001 to November 2004. Before and after therapy thyroid function, thyroglobulin antibody (TGA), thyroid microsomal antibody (TMA) and blood glucose level were measured, and T lymphocyte subsets (CD3, CD4, CD8, CD4/CD8) and NK cells (CD56) were measured by immunofluorescence double labeling monoclonal antibody and flow cytometry, respectively. At the same time, comparison was made with simple GD (15 cases), T2DM (15 cases) and healthy control (20 cases). Results Before therapy, CD4/CD8, CD4 and NK cells in GD/T2DM were less than normal, and there was no significant difference in comparison with simple GD (P<0.05). In T2DM group, only CD4/CD8 and CD4 were less than those of healthy controls (P<0.05). When thyroid function recovered after 1 to 3 months of methimazole treatment in both GD/T2DM and simple GD groups, various indexes recovered, which were more obvious in simple GD. Conclusion Immune hypofunction of GD may be the key to the immune abnormality of GD/T2DM, which is more significant than that of simple GD or T2DM. The recovery of thyroid function and immune abnormality is not consistent, and the recovery of GD is more significant than that of GD/T2DM.     

Role of candesartan against cerebral ischemia and oxidative damage in normotensive rats

Objective Angiotensin Ⅱ (Ang Ⅱ ) contributes to modulating blood pressure by stimulation of Ang Ⅱ AT1 receptors. We devised a rat transient middle cerebral artery occlusion (MCAO) model to assess whether oxidative damage is decreased after pretreatment with Angiotensin Ⅱ AT1 receptor blocker (ARB). Methods After 2 weeks pretreatment with ARB 0. 5 and 1 mg/kg, the male Wister rats were subjected to 2 h middle cerebral artery occlusion (MCAO). At 24 h, the lumen diameter of middle cerebral artery, the plasma level of 8-hydroxy-2'-deoxyguanosine (8-OHdG), and HIF-1 a levels were recorded and compared. Results After pretrcatment with ARB 0.5 and 1 mg/kg, blood pressure did not significantly change compared with that of controls. In the group of candesartan at 1 mg/(kg· day), the lumen diameter was significantly increased compared to that in control group [(86.0±5.0) μm vs. (69.0± 2.1) μm; P<0. 01, n = 6- 8]. The plasma 8-OHdG levels of ARB pretreatment groups were decreased. In immunohistochemical findings, 8-OHdG- and HIF-1α-containing cells in ARB pretreatment groups were decreased. Conclusion Brain ischemia and oxidative damage can be reversed by AT1 receptor blockade in normotensive rats after transient cerebral artery occlusion.     

丘陵地区道路桥梁设计探讨

结合我国丘陵地区道路特点,介绍丘陵地区桥梁设计中跨径的选择原则,墩、台、基础和结构体系宜采用的形式以及桥梁和路基之间的关系,并对丘陵地区道路桥梁设计中存在的问题和相应措施进行探讨,给丘陵地区道路桥梁设计提供参考。     

基于工作流与Agent技术的设施管理系统研究

介绍了工作流技术、工作流管理系统、Agent以及多Agent系统的特点,在此基础上将工作流和Agent技术应用到设施管理系统的构建中,给出了工作流与Agent协同管理的方法和框架,并提出了设施管理系统的三层分布式体系结构,即:用户界面层;应用逻辑层及数据服务层.可为后续设施管理系统的开发提供技术上的参考和借鉴.     

应用流式细胞术检测17β-雌二醇对H2O2诱导的视网膜神经细胞凋亡的保护作用

目的用流式细胞技术(FACS)检测H2O2诱导的视网膜神经细胞凋亡以及17β-雌二醇(βE2)对细胞的保护作用。方法取新生24 h内的SD大鼠进行视网膜神经细胞的原代培养,待细胞培养4-5 d,经不同因素处理后,用MTT法检测细胞存活率,用FACS法检测细胞凋亡率。结果200μmol/L的H2O2能有效诱导视网膜神经细胞凋亡;低浓度的H2O2不能诱导细胞凋亡,但高浓度的H2O2诱导细胞凋亡的同时也导致了正常细胞比率的锐减;10μmol/L的βE2能有效对抗H2O2诱导的视网膜神经细胞凋亡;低浓度的βE2对细胞未表现出保护作用,高浓度的βE2对细胞反而表现出毒性作用。结论一定浓度的βE2在一定范围内能对抗H2O2诱导的视网膜神经细胞凋亡,表现对神经细胞保护的作用。     

Singular perturbation composite control of a free-floating flexible dual-arm space robot

The Free-floating Flexible Dual-arm Space Robot is a highly nonlinear and coupled dynamics system. In this paper, the dynamic model is derived of a Free-floating Flexible Dual-arm Space Robot holding a rigid payload. Furthermore, according to the singular perturbation method, the system is separated into a slow subsystem representing rigid body motion of the robot and a fast subsystem representing the flexible link dynamics. For the slow subsystem, based on the second method of Lyapunov, using simple quantitative bounds on the model uncertainties, a robust tracking controller design is used during the trajectory tracking phase. The optimal control method is designed in the fast subsystem to guarantee the exponential stability. With the combination of the two above, the system can track the expected trajectory accurately, even though with uncertainty in model parameters, and its flexible vibration gets suppressed, too. Finally, some simulation tests have been conducted to verify the effectiveness of the proposed methods.     

内河航道横流对船舶航行的影响

为了使船舶能安全通过航道内的横流区域, 利用水槽进行遥控自航船模试验, 提出了内河航道横流对船舶航行横漂速度、漂角、航迹带宽度和漂距影响的经验公式, 分析了Ⅳ与Ⅴ级航道横向流速的限值范围。分析结果表明: 横流对船舶航行的影响程度主要与对岸航速成反比, 与横流的大小及区域长度成正比, 与船型大小(航道等级)成反比, 同时与驾驶员的航行经验和初始船位有关; 在限制航路航行方式过程中, Ⅳ与Ⅴ级航道对岸航速为2、3、4m·s^-1时, 可克服的一个船长内横流限值为0.48、0.58、0.70m·s^-1。     

A data model applied to the knowledge-based ICAS

Image-guided computer aided surgery system (ICAS) contributes to safeness and success of surgery operations by means of displaying anatomical structures and showing correlative information to surgeons in the process of operation. Based on analysis of requirements for ICAS, a new concept of clinical knowledge-based ICAS was proposed. Designing a reasonable data structure model is essential for realizing this new concept. The traditional data structure is limited in expressing and reusing the clinical knowledge such as locating an anatomical object, topological relations of anatomical objects and correlative clinical attributes. A data structure model called mixed adjacency lists by octree-path-chain (MALOC) was outlined, which can combine patient's images with clinical knowledge, as well as efficiently locate the instrument and search the objects' information. The efficiency of data structures was analyzed and experimental results were given in comparison to other traditional data structures. The result of the nasal surgery experiment proves that MALOC is a proper model for clinical knowledge-based ICAS that has advantages in not only locating the operative instrument precisely but also proving surgeons with real-time operation-correlative information. It is shown that the clinical knowledge-based ICAS with MALOC model has advantages in terms of safety and success of surgical operations, and help in accurately locating the operative instrument and providing operation-correlative knowledge and information to surgeons in the process of operations.     

后张预应力混凝土梁管道摩阻测试研究

介绍了预应力管道摩阻损失的测试原理与测试方法,结合后张预应力混凝土简支梁和连续梁的管道摩阻实测结果,用最小二乘原理识别管道摩阻系数和偏差系数,分析了测试方法的合理性和试验结果的可靠性。探讨目前长束单端张拉中,在千斤顶行程不足的情况下,准确获得预应力钢束中预张力的方法,并将实测结果与设计和施工规范要求进行了对比分析,得出了一些有价值的结论。