羊瘙痒因子263K经灌胃感染仓鼠动物模型的建立

目的　胃肠感染是大多数动物类传染性海绵状脑病(TSE)传播的最主要途径。建立羊瘙痒因子263K灌胃感染仓鼠动物模型,并研究其发病特征。方法　Scrapie 263K毒株低剂量和高剂量经灌胃途径接种金黄仓鼠,在终末期取脑组织,用HE染色观察病理变化,免疫组化法检测PrPSc 的组织沉积特点, Western blot 检测PrPSc 分子特征。结果　低剂量灌胃组仅一只动物发病,高剂量组所有接种动物均发病,潜伏期较颅内注射组明显延长。在脑组织中观察到典型的病理改变;PrPSc免疫组化检测显示弥漫性沉积于脑组织,主要累及脑干、大脑皮层深层、小脑,基本与病理学改变累及的区域一致;Western Blot检测发现感染动物脑提取物出现可抵抗蛋白酶消化的PrPSc条带,与颅内注射组脑组织提取物中PrPSc电泳性状完全一致。结论　羊瘙痒因子263K灌胃可成功引起动物发病,与颅内注射比较,潜伏期、神经病理变化和PrPsc沉积均有不同,可能受到感染途径的影响。     

胎膜组织氧化应激状态与胎膜早破的相关性

目的通过对胎膜早破孕妇胎膜组织中的氧化应激指标———超氧化物歧化酶(SOD)、丙二醛(MDA)及维生素E(Vit E)的测定,探讨胎膜组织的氧化应激与胎膜早破的关系。方法用化学比色法对30例胎膜早破孕妇和30例正常孕足月妇女胎膜组织中SOD、MDA及Vit E进行测定。结果与正常孕足月妇女相比,胎膜早破孕妇胎膜组织中MDA水平均升高(P<0.05),而SOD、Vit E水平均下降(P<0.05,P<0.05)。结论胎膜组织的氧化应激与胎膜早破具有相关性。     

均匀火焰包围下液化气容器的瞬态温度场分析

针对均匀火焰包围下的球型液化气容器的热响应问题，根据传热学原理确定了液化气容器的热边界条件，并结合PLGS99对该边界条件进行了计算，根据求得的边界条件运用ANSYS软件计算了该边界条件下的温度场分布并进行了分析．结果表明对于均匀火焰包围下的球形液化气容器，容器的最大温度梯度出现在最高液面附近，液面的波动是导致温度梯度很大的原因．     

国产盐酸拓扑替康治疗晚期卵巢癌的疗效观察

目的评价国产盐酸拓扑替康治疗晚期卵巢癌的疗效和安全性。方法对84例晚期卵巢癌患者采用盐酸拓扑替康1.25 mg/m2静滴1-5 d,每21 d重复。治疗2周期后评价疗效,有效病例4周后确认疗效。结果84例中可评价疗效72例,可评价毒性84例。完全缓解2例,部分缓解14例,总有效率22.2%,其中初治和复治患者有效率分别为25.0%和20.8%。主要毒副作用为中性粒细胞减少和白细胞减少,两者III/IV度毒性分别为26.1%和26.1%。非血液学毒性轻微。结论国产盐酸拓扑替康单药治疗晚期卵巢癌,特别是复发病例疗效较好,耐受性良好。     

川芎嗪对成体大鼠脑缺血再灌注损伤后海马齿状回细胞增殖的影响

目的研究川芎嗪(tetramethylpyrazine,TMP)对大鼠脑缺血再灌注损伤后海马齿状回(dentate gyrus,DG)细胞增殖的影响。方法成年雄性SD大鼠行2 h大脑中动脉阻塞手术,术后2 h开始腹腔注射TMP[40 mg/(kg.d)]。手术后腹腔注射5-溴脱氧尿核苷(5-bromodeoxyuridine,BrdU),末次注射24 h后处死动物,免疫组化染色观察TMP对脑缺血再灌注损伤后DG细胞增殖的作用。结果正常组和假手术组在DG有少量BrdU阳性细胞,对照组缺血后1 d阳性细胞开始增加,14 d达到高峰(P<0.05),TMP治疗组缺血后1 d损伤侧BrdU阳性细胞数开始增加,7 d达高峰(P<0.05)。结论TMP能促进缺血再灌注损伤大鼠海马齿状回内源性神经干细胞增殖。     

基于多属性效用函数的工程合同索赔机理分析

构造了以工程投资、工期和工程质量为多属性变量的甲乙双方的多属性效用函数。以积式函数为目标函数，通过对博弈Nash解的数学分析得出了达成协议的合同平衡点处双方效用值的关系及其影响因素。根据效用平衡点的变化分析了索赔产生的机理，并从理论上解释了现实中工程界存在的“中标靠低价，盈利靠索赔”现象。     

Joint Grid System Used in Embedding Technique

The joint grid system, which consists of the developed collar grid, the virtual grid anti other grlas, was used in the embedding technique to solve the problem of finding interpolating cells of the internal and external boundary points near the joint regions. With different boundary plane generated along different fixed surface, the collar grid obtained using hyperbolic partial equations can ensure to generate high-quality grids and to provide real interpolating cells for the boundary points in the blanking regions. The virtual grid was used to convert solid wall boundary conditions into an interface condition, however, no fluid flow computations were conducted within the virtual grid. The computational result of body-strake-wing shows that the current developed embedding technique with joint grid system can effectively treat the geometry and can more accurately predict the flow over complexcon-figuration with intersecting surfaces.     

An Experimental Study on the Drag Property of High Temperature Particles Falling into Cold Liquid Pool

This experiment is to study the special resistant induced by the high-speed evaporation surrounding the moving high-temperature particles. An observable equipment was designed, in which the first 11 experiments were performed by pouring one or several Zirconia spheres with various high-temperature and a diameter of 3-10mm into a water pool. The particles falling-down speeds were recorded by high-speed photographic instrumentation,and pressures and water temperatures were measured. A comparison between the experiments with cold and hot spheres respectively, employing three different sphere types each, was presented. The experimental data, com-pared with the theory of the evaporation drag model, are nearly identical.     

基于加速度计的无陀螺捷联惯性制导系统设计

针对舰炮武器系统射击精度低的问题,研究了适宜在炮弹中应用的基于微加速度计的无陀螺捷联惯性制导系统.采用三种方法对载体的角速度进行解算并分析了各种方法的利弊,提供了载体运动参数解算模型,通过仿真计算验证无陀螺方案的可行性,为惯性制导炮弹的发展提供了参考.     

SBS改性沥青混合料损伤模型分析

通过SBS改性沥青混合料的单轴拉伸试验,分析了粘弹性模型与损伤模型的特性,并耦合成粘弹性连续损伤模型;从能量的角度考虑了SBS改性沥青混合料的疲劳损伤特性和损伤效应,并建立了一种与拉伸应变水平相关的疲劳损伤模型。模型与试验吻合较好,可预测不同拉伸应变水平下SBS改性沥青混合料的疲劳寿命。