螺旋进气道铸造缺陷对气道性能影响的试验研究

在稳流气道试验台上，模拟了YC6108柴油机进气道倾斜、纵向胀大等铸造偏差，利用Ricardo和FEV气道性能评价指标，研究铸造缺陷如何影响进气道的性能。试验结果表明：进气道入口处向抬高，涡流强度减小，给向胀大使进气道的流量系数和涡流比明显降低，而当进气道既倾斜又胀大时，将进一步恶化气道性能。     

轮式工程机械机架有限元分析系统

通过对工程机械机架的力学模型和数学模型的研究，开发了适用于微机上运行的工程机架有凶分析系统（ＣＳＦＡＳ）。系统引入了空间薄壁杆件的约束扭转。与通用有限元软件相比，该系统的计算结果更接近实际情况。     

Poisson noise removal based on nonlocal total variation with Euler’s elastica pre-processing

An enhancement-based Poisson denoising method for photon-limited images is presented. The noisy image is firstly pre-processed for enhancing incomplete object information, and then it is denoised while preserving the restored structural details. A variational regularization model based on Euler’s elastica (EE) is proposed for image enhancement pre-processing. A nonlocal total variation (NLTV) regularization model is then employed in the second stage of image denoising. The above two optimization problems are solved by the alternating direction method of multipliers (ADMM). For Poissonian images with low image peak values, experiments demonstrate the validity and efficiency of the proposed method for both restoring geometric structure and removing noise.     

复杂电磁环境下装备指挥效能评估分析

应用模糊综合评判法,通过定性评估与定量评估相结合的分析方法,对复杂电磁环境下的装备指挥效能评估进行了研究.针对复杂电磁环境下装备指挥的特点,设计了装备指挥效能评估指标体系模型,通过分析计算得出的结果表明,模糊综合评判法应用于复杂电磁环境下装备指挥效能评估是可行的.     

汽车制动引起转向盘抖动问题分析

采用模态分析方法得到汽车制动引起转向盘抖动的原因是前悬架系统模态;通过改变控制臂后衬套刚度可改变悬架系统模态。试验结果表明:增加下摆臂后衬套刚度可改变悬架系统模态,有效缩短振动频域,降低转向盘抖动幅度。     

铁路隧道松散破碎围岩塌方数值模拟分析

运用二维离散元程序UDEC3.1软件,对3种不同顶板岩性组合模型进行数值模拟,再现了隧道顶板岩层离层、弯曲、沉降、开裂直至塌方的全过程,分析了松散破碎围岩隧道塌方后围岩的位移、应力和塑性区分布特征。     

浅谈铁路客运自动化

为旅客提供高效、优质的服务是铁路客运服务的最终目标。客运自动化系统具有及时、安全、资源共享和管控一体等优势,已成为车站客运信息系统的建设方向。分析了我国铁路客运信息系统的现状,阐述了铁路车站客运自动化建设的意义、目标和系统组成等。     

三峡库区船舶碰撞能量损失方法的研究

采用外部碰撞力学方法计算2艘2 000 t级化学品船以不同速度,不同碰撞角,在不同碰撞点相撞后的能量损失,并利用显示动力有限元软件LS_DYNA模拟2艘化学品船在能量损失最大情况下碰撞的响应.     

我国港口企业要加快“走出去”的步伐

“走出去”和“引进来”都是利用国际资源更高级的形式。港口是我国国内(不包括港、澳、台,下同)交通部门中,最早引进外资的领域。在引进来方面,十几年来,中外合资的港口已遍布全国,新建的集装箱码头除洋山一期外,几乎都有外资的介入,这些对我国港口的发展起了巨大的推动作用,保证了我国在加入WTO 后对外贸易的飞速增长,使港口成为开辟国内外两个市场,利用国内外两种资源的有力工具。但在“走出去”上,我国虽已经启动了走向国际港口市场的步伐,两个大型航运企业已经在国外投资建设了自己的码头。但与我国国家实力,我国港口企业的实力和我国港口在     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.