EB1基因表达与胚胎染色体数目异常的关系

目的研究EB1基因在胚胎绒毛细胞中的表达,并分析其对染色体分离的影响。方法采用荧光定量PCR技术检测EB1基因在人工流产绒毛核型分析正常的胚胎细胞和自然流产绒毛核型分析异常的胚胎细胞中的表达差异;免疫组织化学方法检测EB1蛋白在两者中的表达情况,并用Western blot加以验证。结果EB1基因mRNA拷贝数/GAPDH基因mRNA拷贝数的均值在人工流产绒毛核型分析正常的胚胎细胞中为0.010 04±0.008 223,在自然流产绒毛核型分析异常的胚胎细胞中为0.150 9±0.063 3。Western blot和免疫组织化学结果表明其在自然流产绒毛核型分析异常胚胎细胞中的表达也高于其在人工流产绒毛核型分析正常胚胎细胞中的表达。经统计分析,EB1基因mRNA水平和蛋白水平在上述两组细胞中的表达均有显著差异。结论EB1基因在自然流产绒毛核型分析异常的胚胎细胞中的表达明显高于其在人工流产绒毛核型分析正常的胚胎细胞中的表达,其高表达可能导致染色体分离异常。     

滑油运动粘度对尾轴轴颈振动的影响研究

船舶尾轴的振动与尾轴承油膜的动态支承刚度和阻尼密切相关,互相耦合,相互激励,影响船舶航行安全性.润滑油运动粘度是影响润滑油膜的动态特性的重要因素之一,粘度的改变对尾轴的振动行为产生重要影响.文中从动力学方面研究了滑油粘度对尾轴轴颈振动的影响,得出提高润滑油粘度有利于抑制轴颈振动的结论.     

角膜基质脱细胞处理与相容性实验研究

目的寻找有效适宜的脱细胞方法对猪角膜进行处理,制备角膜生物支架材料。方法利用胰酶和胶原酶消化结合液氮冻融的方法对猪角膜基质进行处理,60Co消毒,冻干保存。经组织学观察、细胞培养附和与动物移植检验其生物特性。结果获得的脱细胞角膜基质,显微结构观察显示板层结构完整,胶原纤维间组织结构疏松,未见细胞残留,整个基质成网状结构,在上皮面保存有完整连续的基底膜。细胞培养附和实验观察到种子细胞可以在基质上良好生长。动物移植实验未见明显的炎症反应与排斥。结论经该法脱细胞处理后得到的角膜基质无细胞毒性,组织相容性好,可以成为适宜的组织工程支架材料。     

基于岩石非线性统一强度理论的地下洞室弹塑性分析

采用岩石非线性统一强度理论考虑了岩体的塑性软化特性,利用衬砌与围岩的位移协调条件,对地下圆形洞室进行了弹塑性分析,获得了圆形洞室的应力,洞室变形及塑性区半径的解,这些解既考虑了岩石拉压强度相差较大的特点,又考虑了中间主应力效应及其区间性;既反映了岩石的非线性破坏特征,又可以推广到岩体及节理岩体之中去,有一定的理论及工程应用价值.     

西部红层软岩地区高速公路路堑与隧道方案评价

针对红层软岩的工程性质,利用广泛的调查研究资料,分析了高速公路深挖路段的技术经济影响因素,构建了路堑与隧道方案评价的指标体系,并利用灰色关联度作为测度进行综合评价,得到了路堑与隧道相比的合理开挖深度,为西部红层软岩地区高速公路的设计和施工提供有益参考.     

浅谈就地冷再生技术在营口地区的应用

就地冷再生技术在辽宁特别是营口地区已得到广泛应用，并逐渐被交通领导部门推广，从辽宁省就地冷再生的首例试验路为基础，介绍了就地冷再生的应用过程。     

公交车车载视频监控系统解决方案探讨

介绍了公交车车载视频监控系统的组成及功能设计方案。     

Structural Optimization of Hatch Cover Based on Bi-directional Evolutionary Structure Optimization and Surrogate Model Method

Weight reduction has attracted much attention among ship designers and ship owners. In the present work, based on an improved bi-directional evolutionary structural optimization (BESO) method and surrogate model method, we propose a hybrid optimization method for the structural design optimization of beam-plate structures, which covers three optimization levels: dimension optimization, topology optimization and section optimization. The objective of the proposed optimization method is to minimize the weight of design object under a group of constraints. The kernel optimization procedure (KOP) uses BESO to obtain the optimal topology from a ground structure. To deal with beam-plate structures, the traditional BESO method is improved by using cubic box as the unit cell instead of solid unit to construct periodic lattice structure. In the first optimization level, a series of ground structures are generated based on different dimensional parameter combinations, the KOP is performed to all the ground structures, the response surface model of optimal objective values and dimension parameters is created, and then the optimal dimension parameters can be obtained. In the second optimization level, the optimal topology is obtained by using the KOP according to the optimal dimension parameters. In the third optimization level, response surface method (RSM) is used to determine the section parameters. The proposed method is applied to a hatch cover structure design. The locations and shapes of all the structural members are determined from an oversized ground structure. The results show that the proposed method leads to a greater weight saving, compared with the original design and genetic algorithm (GA) based optimization results.     

Multi-Band Hybrid Mobile Phone Antenna Based on Electromagnetic Coupling

This research presents a multi-band hybrid mobile phone antenna based on electromagnetic coupling,which can be applied to mobile handheld devices, occupying a small board space of 39.7 mm × 15.6 mm on the system circuit board. By adding resonant and coupled branch instead of multi-feed on the traditional bent antenna,this design provides four wide operating bands of 0.772—0.998 GHz, 1.540—1.600 GHz, 1.680—2.270 GHz and2.300—2.690 GHz with the hybrid feature of planar inverted-F antenna(PIFA), L-shape, U-shape and S-shape structures, which cover nine-band, i.e., GSM850, GSM900, GPS1575, DCS1800, PCS1900, IMT200, LTE2300,LTE2600 and Blue Tooth/Wi-Fi. Ansoft software HFSS is used in this research to make the antenna performance better and the operating principle of the proposed antenna is described in detail. Result of simulation reveals that the maximum gains of these four wide bands are 2.20,-0.99, 4.01 and 4.05 dBi, respectively. Moreover, this research also tests the return loss(S11) of the fabricated antenna with the vector network analyzer and the result is in accordance with the simulation result on the whole. There are four wide resonant frequencies which cover nine-band of wireless wide area network(WWAN), wireless local area network(WLAN) and long term evolution(LTE), when the available bandwidth is better than 6 d Bi return loss.     

中等城市常规公交线网连通度计算方法

为了更合理的评价公交线网的结构特性,将网络分析中的连通度指标引入中等城市常规公交路网的分析评价中。分析常规公交路网连通度的一般计算方法,并结合用地类型确定线路的权重,提出公交线网加权连通度的计算方法,并进行示例演示。