夫妻配对MTHFR基因型分布与不明原因反复流产的关系

目的　探讨亚甲基四氢叶酸还原酶 (MTHFR)基因多态性在夫妻间的不同存在形式与不明原因反复流产之间的关系。方法　采用聚合酶链反应 限制性片段长度多态性 (PCR RFPL)的方法 ,对 32对有两次及两次以上不明原因流产史的夫妇和 39对健康夫妇进行MTHFR6 77C→T突变相关性研究。结果　病例组与正常对照组流产发生的危险性比较 ,夫妻双方都是纯合或杂合突变型 ,其基因型分布与流产发生的危险性增高高度相关 ,OR =2 94 1,95 %CI[1.0 6 2～ 8.133];夫妻双方有一人是纯合或杂合突变型 ,而另一人为野生型的 ,其基因型分布与流产发生的危险性无关联 ,OR =0 .819,95 %CI[0 .32 0～ 2 .0 94 ];夫妻双方都是野生型 ,其基因型分布与发生流产的危险性降低高度相关 ,OR =0 188,95 %CI[0 .0 4 6～ 0 .76 0 ]。结论　子代携带MTHFR纯合或杂合突变可能是胚胎早期发育异常的原因之一 ,从而导致流产的发生。     

岩体温度场与应力场耦合作用的一种量化方法

简要分析了温度场与应力场的耦合机理，从岩石热－弹性作用和温度扩散方程出发详细介绍了热－弹性耦合强度量化参数PTC的计算方法。计算了几种常见岩石的热－弹性耦合强度，结果表明，岩石的热－弹性耦合强度一般处于10^-3量级。同时，就提出的无量纲参数PTC在工程应用中的实用性及意义进行了初步探讨。     

西安市大气悬浮颗粒物致突变性及其金属特征

目的　了解西安市采暖期和非采暖期的大气总悬浮颗粒物 (TSP)浓度、有机提取物的致突变性及无机提取物中Pb、Mn、Cr、Ni、Cd等金属元素特征。方法　根据西安市城市功能分区 ,用网格式布点法选择了 4个大气质量监测点 ,于采暖期和非采暖期共采集大气悬浮颗粒物样品 96份 ,分别采用增重法、Ames试验—平板掺入法和火焰原子吸收法 ,对样品中大气TSP浓度、有机提取物的致突变性及无机提取物中Pb、Mn、Cr、Ni、Cd等金属元素含量进行测定。结果　西安市采暖期大气TSP浓度为 0 .5 865mg·m-3,非采暖期为 0 .3 5 3 5mg·m-3(P <0 .0 5 ) ,且两期TSP浓度均显著高于其各自对照区 (P <0 .0 5 )。各采样点TSP的有机提取物致突变性检测结果显示各功能区均可检出致突变阳性样品 ,同一季节各监测点致突变阳性检出率无显著性差异 (P >0 .0 5 ) ,但采暖期致突变阳性检出率显著高于非采暖期 (P <0 .0 5 )。各采样点TSP无机提取物中 5种金属元素浓度由高到低依次为Pb、Mn、Cr、Ni、Cd。采暖期各金属元素浓度显著高于非采暖期 (P <0 .0 5 )。结论　西安市大气TSP水平与采暖燃煤关系密切 ,燃煤可能是造成西安市大气TSP致突变性增强的主要原因之一     

冷处理对烧结钕铁硼磁性能的影响

研究了烧结钕铁硼永磁体经液氮冷处理后的磁性能变化规律。35SH、35H、N40、N35四个牌号的烧结钕铁硼永磁体经液氮冷处理后,磁性能有不同程度的提高。     

Study on lift-up speed of aerodynamic compliant foil thrust bearings

Objective The experimental study on the lift-up speed of a new kind of compliant aerodynamic foil thrust hearings was performed on the multifunetional test rig established for testing the performances of foil gas bearings. Methods The lift-up speed of foil gas thrust hearing under given axial load was analyzed through the spectrum of axial displacement response in frequency domain. Results The test results indicated that the difference in the spectrum of axial displacement responses before and after lifting up of the rotor was obvious. After lifting up of the rotor, there were only larger components of rotation frequency and lower harmanie frequencies. If the rotor wasn＇t lift-up, there were also larger components of other frequencies in the spectrum. Conclusion So by analyzing the spectrum of axial displacement response, the results showed that the lift-up speed was about 1 860 rpm when the axial load was 31N.     

大连市后盐立交桥门架标志的设计与应用

浅谈后盐立交桥采用大跨度门架标志的必要性和该门架标志的结构特点、安全技术措施,以及门架标志在现代公路设计中广泛应用的前景。     

Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.     

我国铁路高速事业的发展

介绍了我国高速铁路的建设情况，同时也介绍了我国高速列车车辆的关键技术包括流线型，轻量化，制动系统，转向架系统，安全技术及车体材料等。     

城市轨道车辆的自动控制

本文概述了城市轨道车辆的运行特点;节能的最佳运行方式;功率电子技术在驱动装置上的应用;城市轨道车辆自动控制的分类及主要任务;微型计算机在城市轨道车辆自动控制方面的应用;典型自动控制设备的特点.     

基于可持续发展的城市交通网络定量设计研究

基于可持续发展思想，提出可持续发展的城市交通网络宏观优化模型，对网络设计中的路段变量进行宏观控制具有一定的指导意义。