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271.
目的探讨FHIT基因表达改变和HPV16感染与人宫颈癌发生的关系。方法采用反转录-巢式聚合酶链反应方法测定5种人宫颈癌细胞株(SiHa、HeLa、RJC-1、CS1213、C4-1)和58例宫颈癌组织与18例正常宫颈对照中FHIT mRNA的表达;回收7例FHIT基因不同的转录扩增产物,纯化后进行DNA测序;PCR技术检测组织中HPV16型的感染状况。结果SiHa、HeLa和C4-1宫颈癌细胞中有FHIT基因转录异常;宫颈癌组织中39例(67.2%)存在FHIT基因异常表达,显著高于正常对照组0例(0%)(P<0.05);37例(63.8%)有HPV16感染,显著高于正常对照组1例(5.0%)(P<0.05)。宫颈癌组织中有HPV16感染患者的FHIT基因表达异常数(30/37)显著高于HPV16未感染的患者(9/21),二者之间存在相关性(P<0.01);FHIT基因的异常表达和HPV16的感染与患者的年龄、临床分期、肿瘤直径、病理分级及是否伴淋巴结转移无相关性(P>0.05)。序列分析发现FHIT基因转录本主要存在不同程度的外显子的缺失,以第5位和第6位外显子的缺失为主,未见未知序列的插入和点突变。结论FHIT基因在人宫颈癌组织中的异常表达率明显增高,且与HPV16的感染有关,这些改变可能在人宫颈鳞癌的发生中起着重要作用。 相似文献
272.
Construction of diaphragm wall panels may cause considerable stress changes in heavily overconsolidated soil deposits and can induce substantial ground movement. The 3D Lagrangian method was adopted to model the mechanical response of ground, including horizontal normal stress and shear stress, lateral ground displacement and vertical ground surface settlement, during the slurry trenching and concreting of diaphragm wall panels. Numerical results show that slurry trenching leads to horizontal stress relief of ground, reducing the horizontal stress of the ground from initial K0 pressure to hydrostatic betonite pressure. Wet concrete pressure lies between the hydrostatic bentonite pressure and the initial K0 pressure, so it can compensate partially the horizontal stress loss of the ground adjacent to the trench and thus reduce the lateral movement of the trench face as well as the vertical settlement of the ground surface. 相似文献
273.
为了解强震作用下我国斜拉桥桥塔的抗震性能以及震害现象,通过调研确定典型斜拉桥原型,对其H型混凝土桥塔进行纵桥向振动台试验研究.基于斜拉桥的动力特点及振型分析方法进行桥塔模型的简化设计、制作和安装,输入不同类型及不同强度的地震波,对桥塔模型进行了振动台试验.结果表明:相比一般的Elcentro波和Chichi地震波,相同加速度峰值地震输入下,场地人工波激起桥塔的地震反应最剧烈,且随着地震动输入的不断加强,桥塔下塔柱、中塔柱靠近下横梁部位先后不同程度地出现了裂缝开展延伸现象,其中塔底向上30 cm区域内损伤较明显,存在多条贯通裂缝,塔底截面最外侧钢筋屈服,模型整体刚度有明显下降趋势,但桥塔抗震性能依然表现良好. 相似文献
274.
基于城际铁路与市域快轨在决策体系和功能技术上的现状背景,分析深圳、香港两地的都市圈轨道交通建设实践案例,旨在提出统筹空间规划、轨道交通规划与工程设计的决策支持框架。从出行人性化、空间相契合、经济可持续三方面阐述正确处理城际铁路和市域快轨关系应考虑的三方面因素。进而提出涵盖车站、线路、土地利用、周边接驳、投融资等方面的规划决策分析框架。强调城际铁路和市域快轨的功能要落实在都市圈的空间规划上,通过全程旅行速度来控制速度目标值的实现,进而确定车辆选型、供电制式、站间距等设计要素。 相似文献
275.
276.
In this paper, a numerical investigation of a float-over installation for an offshore platform is presented to verify the feasibility of the actual installation. The hydrodynamic performance of a T-barge is investigated in the frequency domain, and the coupled motions are analyzed in the time domain. We then compare with those of the model test and determine that the response amplitude operator and the time series agree quite well. The barge exhibits favorable hydrodynamic behavior in the considered sea state, and the equipment loads are allowable. The mooring system and sway fender forces are within the permissible range. Based on these results, we can verify that the actual installation of the offshore platform is feasible. We accurately simulated many important factors and effectively reduced the risk associated with the offshore installation, which is of great importance. As such, we demonstrate that the numerical simulation of the float-over installation for offshore platforms has practical engineering significance. 相似文献
277.
Seyed Masoud Mahmoudof Peyman Badiei Seyed Mostafa Siadatmousavi Vahid Chegini 《船舶与海洋工程学报》2018,17(1):140-151
This study evaluates the capability of the Simulating WAves Nearshore (SWAN) wave model (version 41.01) in predicting significant wave height and spectral peak energy content for swell waves in very shallow water of surf zone during depth-induced wave breaking and dissipation. The model results were compared with field measurements at five nearshore stations. The results demonstrated that some breaker index formulations were successful for significant wave height prediction in surf zones. However, an incorrect shape of the energy spectrum and overestimated near spectral peak energy content at shallow water stations were obtained using all of the embedded depth-induced wave breaking formulations in SWAN. The dependent breaker index on relative depth (Kpd) formulation, which was successful in predicting near spectral peak energy content, resulted in an average error of 30%. Finally, this formulation was modified to enhance the model performance in reproducing the spectral peak energy content. 相似文献
278.
A numerical study of ship-to-ship interaction forces is performed using a commercial CFD code, and the results are compared with experimental data and with the results of a panel method analysis. Two ship models have been used in the interaction forces analysis: a tug and a tanker, advancing parallel to each other with different lateral distances and two different values of the fluid depth. Computations are carried out with four different flow models: inviscid and viscous flow with the free surface modeled as a rigid wall and inviscid and viscous flow with the deformable free surface. A fair agreement was obtained with available experimental data and results obtained by panel method. The influence of viscosity in the computations is found to be comparatively weak, while the wavemaking effects may be important, at small magnitude of the horizontal clearance. 相似文献
279.
In this work, trapped mode frequencies are computed for a submerged horizontal circular cylinder with the hydrodynamic set-up involving an infinite depth three-layer incompressible fluid with layer-wise different densities. The impermeable cylinder is fully immersed in either the bottom layer or the upper layer. The effect of surface tension at the surface of separation is neglected. In this set-up, there exist three wave numbers: the lowest one on the free surface and the other two on the internal interfaces. For each wave number, there exist two modes for which trapped waves exist. The existence of these trapped modes is shown by numerical evidence. We investigate the variation of these trapped modes subject to change in the depth of the middle layer as well as the submergence depth. We show numerically that two-layer and single-layer results cannot be recovered in the double and single limiting cases of the density ratios tending to unity. The existence of trapped modes shows that in general, a radiation condition for the waves at infinity is insufficient for the uniqueness of the solution of the scattering problem. 相似文献
280.
Guro Persdotter Fjeld Sturle Danielsen Tvedt Helle Oltedal 《WMU Journal of Maritime Affairs》2018,17(4):475-495
The present review examines the research literature on Non-Technical Skills (NTS) used by ships’ bridge officers in connection with navigation. The aim of the study was to (i) identify the cognitive and interpersonal skills which have been the focus of previous studies and (ii) explore how the content of these skills has been described. Databases searched included Academic Search Premier, PsycINFO, Science Direct, and Web of Science. Nineteen studies were included in the review. Five NTS were identified: situation awareness (SA), decision-making (DM), workload management (WM), communication, and leadership. In addition to discussing each skill, the review raises four overarching issues with the present literature for the bridge domain: (1) Have all the relevant skills been subject to exploration? (2) Have the skills identified been explored in detail? (3) There seems to be an uneven distribution of research between cognitive and interpersonal skills. (4) There is little research into understanding the skills as a complete taxonomy. Knowledge on how the skills are linked and interplay with one another is incomplete. Overall, further research on all these aspects of NTS in the maritime domain could increase scientific understanding and contribute to bridge operational practice and to the further development and evaluation of NTS training such as Bridge Resource Management (BRM). 相似文献