快速老化小鼠的学习记忆缺陷及其生化机制

目的用快速老化小鼠(SAMP8)模拟老年痴呆,观察其学习记忆能力,并探讨其学习记忆能力下降的可能机制。方法采用10月龄SAMP8,并用同龄抗快速老化小鼠(SAMR1)作为正常对照。用水迷路检测近记忆以及空间学习记忆能力。用生物化学方法检测大脑皮层线粒体膜电位和ATP酶活力,观察线粒体功能;检测皮层和海马胆碱乙酰基转移酶(ChAT)和乙酰胆碱酯酶(AChE)的活力,观察中枢胆碱能神经功能;检测血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,观察其全身性氧化应激状况。结果与SAMR1相比,SAMP8出现明显的衰老体征,其近记忆和空间学习记忆能力明显减弱,具有衰老和痴呆特征;皮层线粒体膜电位和ATP酶活力显著降低,海马ChAT活力显著下降,AChE活力显著升高,血清SOD活力稍有增高。结论SAMP8可以很好地模拟老年痴呆,其机制包括大脑皮层线粒体功能降低、海马胆碱能神经功能下降以及氧化应激等。     

早期自然流产患者绒毛组织中核转录因子-kappa B的表达

目的 探讨核转录因子-κB(NF-κB)在绒毛组织中的表达及活化与早期自然流产的关系.方法 采用免疫组化方法检测早期自然流产患者和同期妊娠的健康妇女绒毛组织中NF-κB p65的表达;激光共聚焦显微镜(CLSM)检测NF-κB p65的核区与胞浆区比值(FN/FC)以观察其活化情况.结果 早期自然流产患者绒毛合体滋养层细胞NF-κB表达强度低于正常早期妊娠妇女,其差异具有统计学意义(P<0.001);早期自然流产患者绒毛间质细胞NF-κB表达的FN/FC(0.7602)明显高于正常早期妊娠妇女(0.6977)(t=3.511,P<0.001).结论 NF-κB在早期自然流产患者绒毛组织合体滋养细胞中的低表达以及其在间质细胞中的过度活化可能与早期自然流产的发生有关.     

Simulation and analysis of vehicle stability based on ADAMS/CAR differential brake

To improve the braking safety of automobiles, the author studied the effect of differential brake on the stabilities. To analyze the mechanical characteristics of differential brake, automotive subsystem models were built by applying ADAMS/CAR, and automotive mechanics simulation model was built by setting the main subsystems such as body, engine and brake. The simulation model studied the distribution mode of three kinds of differential brake, and beeline braking stability and turning braking stability were simulated. It shows that differential brake can amend turning shortage of automobile brake and improve its braking stability, but the effect of automobile mass on its braking stability is great. So the distribution mode of braking force and the effect of mass change should be considered while differential brake is applied.     

物流企业联盟利益分配方法研究

分析了物流企业联盟利益分配的原则,提出了几种常用的利益分配方法,并在此基础上进行改进,提出了基于加权群体重心模型的利益分配方法,实例证明该方法更加具有公平合理性,在物流企业联盟的利益分配中有应用推广价值。     

独塔斜拉桥承载能力评估方法

以独塔斜拉桥为研究对象,通过对桥梁结构的外观检测、斜拉索内力测量和结构动、静载试验,并结合有限元模型修正,评定桥梁结构的健康状态和承载能力.结果表明:由于施工过程中斜拉索内力配置的不合理性,改变了原有结构的受力状态,是该桥病害产生的主要成因.     

温拌与热拌SMA沥青混合料路用性能的对比分析

从压实性能、高温性能、低温性能、疲劳性能、水稳定性能、剪切性能及老化性能等方面对熟拌和温拌SMA沥青混合料进行路用性能对比分析,结果表明在降低施工温度节能环保的情况下,热拌和温拌SMA沥青混合料的路用性能相当。     

温拌沥青混合料的使用性能研究

通过室内试验全面分析了温拌沥青混合料的力学性能、高温稳定性、低温抗裂性及水稳定性。并与同级配的热拌沥青混合料进行对比,结果表明二者使用性能相当。     

隧道火灾作用下水泥路面瞬态温度场计算方法

隧道发生火灾时水泥路面板内升温迅速,温度梯度较大,采用数值方法求解路面热传导偏微分方程易导致数值解振荡、计算结果失真,且过程繁琐.根据火灾下水泥混凝土热工参数变化规律及傅里叶余弦变换,推导了隧道火灾下路面内温度场的理论计算公式,并与通用有限元软件计算结果进行了比较.结果表明,推导公式可用于计算连续时间内路面板不同位置的温度分布,较好解决了数值方法求解路面瞬态温度场时数值解振荡、不连续的问题,提高了计算精度,且路面内温度场变化规律呈明显的抛物线型态势,与一维热传导微分方程的物理意义吻合较好.因此,推导公式求解火灾下路面结构瞬态温度场具有更好的适用性.     

基于大规模定制的旅游业顾客保留模型研究

在对大规模定制理论、顾客保留理论进行文献综述的基础上,分析了基于大规模定制的旅游业顾客保留的内涵及影响因素,包括个性化体验质量、定制服务质量、定制价格水平、转移障碍等,构建了基于大规模定制的旅游业顾客保留模型,设计了共18个测量变量,并以中国国旅(武汉)国际旅行社有限公司为实证对象,对构建的理论模型进行了实证研究.     

Correlation of expression of ER, PR and c-erbB-2 with ultrasonographic features in invasive ductal cancer of breast

Objective To analyze the relationship of the expression level of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor-2 (HER-2, c-erbB-2) of breast invasive ductal carcinoma (IDC) with ultrasonographic characteristics. Methods Totally 104 patients with IDCs confirmed pathologically were involved in this study. ER, PR and c-erbB-2 expression in the IDC specimens was determined by immunohistochemical staining technique. The correlation between the ultrasonographic features and the positive expression of ER, PR or c-erbB-2 was analyzed. Results The positive expression rate of ER and PR in the group with tumor spiculation sign and posterior acoustic attenuation was higher than that in the group without. The positive expression rate of ER differed significantly (P<0.05) while that of PR did not (P>0.05). The over-expression rate of c-erbB-2 in the group of microcalcification, sufficient blood flow and axillary lymph node metastasis was higher than that in the group of non-microcalcification, deficient blood flow or without axillary lymph node metastasis (P<0.05). The expression of ER, PR and c-erbB-2 was not related to the size of tumor (P>0.05). Conclusion The expression of ER and c-erbB-2 is closely related to the ultrasonographic characteristics of IDC, which may, to some extent, reflect the expression level of ER and c-erbB-2.