铁路机车车辆工业用热处理数据库的开发

采用Visual Foxpro编程，建立了铁路机车车辆关键零部件热处理数据库系统（HTMIS）。在该系统中，可查询到机车车辆关键零部件在国内外铁路行业所用的材料、热处理、工艺、达到的性能、可能出现的缺陷及其原因和预防补救措施，系统还包括常用材料和热处理标准方面的数据，并且有扩充删除、打印等功能。     

Nonlinear Statistical Process Monitoring Based on Control Charts with Memory Effect and Kernel Independent Component Analysis

A novel nonlinear combination process monitoring method was proposed based on techniques with memory effect (multivariate exponentially weighted moving average (MEWMA)) and kernel independent component analysis (KICA). The method was developed for dealing with nonlinear issues and detecting small or moderate drifts in one or more process variables with autocorrelation. MEWMA charts use additional information from the past history of the process for keeping the memory effect of the process behavior trend. KICA is a recently developed statistical technique for revealing hidden, nonlinear statistically independent factors that underlie sets of measurements and it is a two-phase algorithm: whitened kernel principal component analysis (KPCA) plus independent component analysis (ICA). The application to the fluid catalytic cracking unit (FCCU) simulated process indicates that the proposed combined method based on MEWMA and KICA can effectively capture the nonlinear relationship and detect small drifts in process variables. Its performance significantly outperforms monitoring method based on ICA, MEWMA-ICA and KICA, especially for long-term performance deterioration.     

铜陵港应与城市定位相协调

《铜陵市城市总体规划（2003-2020）》将铜陵市定位为“长江中下游重要的工贸港口城市”；交通部发布的《长江干线航道发展规划（2003-2020）》明确铜陵为万吨级海轮进江的终点港，铜陵作为长江下游南岸的一座临江新兴城市，发展水运，港口有着得天独厚的优势。     

水下挤密砂桩加固机理及沉降计算方法

阐述了挤密砂桩的加固机理,根据国内外相关资料,总结出水下挤密砂桩复合地基的沉降计算方法,并以洋山深水港区三期临时码头接长工程中的挤密砂桩复合地基为例对计算方法进行了验证,与实测沉降量吻合,可为类似工程设计提供参考.     

铁路自动售票机人机交互系统的优化设计与实现

分析铁路自动售票机在人机交互设计方面的现状、存在的问题和不足,结合用户认知负荷和最优化理论,提出了铁路自动售票机人机交互系统的优化方案。实践表明,该方案能够有效减少旅客购、取票时的操作步骤,达到提高效率的目的。     

Methylation status of the interferon-gamma gene promoter in chronic hepatitis B

Objective To evaluate the methylation status at CpG site -55 in the interferon-gamma (IFN-7) gene promoter and its effect on IFN-7 expression in chronic hepatitis B. Method The authors recruited 30 patients with UBeAg-positive chronic hepatitis B (CHB), 30 HBeAg-negative CHB patients, and 30 healthy blood donors. Pyrosequeneing was used to determine the methylation status at CpG site -55 in the IFN-γ gene promoter following bisulfite treatment of DNA in peripheral blood mononuclear cells (PBMCs). The expression of IFN-γ was analyzed by real-time RT-PCR and ELISA. HBV DNA in PBMCs was detected by nested PCR. Results The methylation level at CpG site -55 in the IFN-γ gene promoter was significantly increased, resulting in subsequent down-regulation of the expression of this cytoldne in CHB. The methylation level at CpG site -55 was significantly higher in HBeAg-positive patients than in HBeAg-negative ones (P<0.01) and was also significantly higher in PBMCs from HBV DNA-positive patients than from HBV DNA-negative ones (P<0.01) ; the methylation level at CpG site -55 was positively correlated with the amount of HBV DNA in serum (P<0.01). Oonclusion IFN-γ gene expression appears to be regulated by methylation of the IFN-γ gene promoter in CHB; the methylation level at CpG site -55 is associated with HBV infection.     

基于ADAMS/view行李厢盖弹簧力分析及优化

利用ADAMS/view软件建立行李厢盖多刚体模型,通过动态仿真对行李厢盖开启和关闭过程进行模拟分析,考虑行李厢盖的开启角度、开启力、开启时间、关闭力及关闭速度等设计参数。为改善行李厢盖开启时间过短现象,对弹簧刚度及预载进行优化分析,提升用户使用满意度。     

酵母双杂交系统筛选HBV PreS1相互作用蛋白

目的利用Sos招募系统(SRS),构建含HBV PreS1基因的酵母双杂交诱饵载体,筛选人肝细胞与HBV PreS1蛋白相互作用的蛋白,进一步探讨HBV侵入肝细胞的机制。方法以HBV ayw亚型全长质粒PCP10为模板,PCR扩增HBV PreS1基因,克隆到酵母表达载体pSos中,构建诱饵质粒pSos-PreS1,将其转化cdc25酵母感受态细胞,提取酵母蛋白质进行Western blot分析,证实其在酵母细胞中的表达;将pSos-PreS1分别与pMyr Lamin C、pMyr SB共转化酵母,证实诱饵蛋白无自激活作用。将pSos-PreS1与人肝cDNA文库共转化cdc25酵母感受态细胞,通过营养及温度选择性培养筛选阳性菌落,扩增阳性菌落目的基因并测序。结果成功构建了HBV PreS1基因酵母双杂交诱饵载体pSos-PreS1,筛选得到5个准阳性克隆,序列分析表明其分别与人类钾通道调制因子1(KCMF1)、细胞色素C、VitD结合蛋白、人源去唾液酸糖蛋白受体(ASGPR)和人白蛋白具有高度同源性。结论利用SRS筛选出5个可能与HBV PreS1蛋白相互作用的蛋白,为进一步了解HBV侵入肝细胞的机制奠定了基础。     

HBV preS1基因酵母表达载体的构建及表达

目的 构建HBV preS1基因的酵母表达载体,探讨HBV preS1蛋白的功能.方法 以HBV ayw亚型全长质粒PCP10为模板,聚合酶链反应(polymerase chain reaction, PCR)扩增HBV preS1基因,克隆到pGEM-T载体中命名为T-preS1,以NcoⅠ和MluⅠ双酶切T-preS1后回收与酵母表达载体pSos连接,对重组质粒进行序列测定后命名为pSos-preS1,经醋酸锂法将其转化酵母菌cdc25(a),提取酵母蛋白质进行Western免疫印迹分析.结果 成功构建了HBV preS1基因的酵母表达载体,Western免疫印迹分析显示HBV preS1基因在酵母细胞中正确表达.结论 pSos-preS1的构建为通过SOS招募系统(sos-recruitment system, SRS)筛选与HBV preS1蛋白相互作用的蛋白和进一步探讨HBV preS1蛋白在HBV致病中的作用奠定了基础.     

对氮化层反应扩散机理的探讨

通过对１Ｃｒ１８Ｎｉ９不锈钢盐浴软氮化处理后的氮化层组织特点及生长速度的研究，探讨了氮化过程的反应扩散机理，提出了对该机理进行分析研究的一种思路。